Fig. 3
From: Optimized workflow to modify microRNA expression in primary human intravascular cells
Expression of miR-15a-5p in primary monocytes following unassisted uptake of LNA mir-15a-5p inhibitors. Monocytes were cultured with A LNA-PE or B LNA-PS miR-15a-5p inhibitors and their respective scrambled control at elevated concentration (30, 100, 200 and 600 nM). Following 48 h, total RNAs were isolated and expression levels of miR-15a-5p and reference miRNA miR-103a-3p were measured by qRT-PCR. Relative miR-15a-5p expression was shown as mean ± SEM. N ≥ 3 experiments N ≥ 2 technical replicates were performed