Fig. 4

Expression of miR-15a-5p and miR-20b-5p in primary ECs following transfection of the respective miRNA mimics. ECs were transfected with mirVana miRNA mimic for A miR-15a-5p and C miR-20b-5p or their respective scrambled control at elevated concentration (0.45, 1.5, 5 and 16.7 nM) using Lipofectamine RNAiMAX as a carrier. In addition, ECs were transfected with LNA miRNA mimic for B miR-15a-5p and D miR-20b-5p or their respective scrambled control at elevated concentration (0.45, 1.5, 5 and 16.7 nM) using Lipofectamine RNAiMAX as a carrier. Following 48 h, total RNAs were isolated and expression levels of miR-15a-5p, miR-20b-5p and reference miRNA miR-103a-3p were measured by qRT-PCR. Relative expression levels of miR-15a-5p and miR-20b-5p in comparison to miR-103a-3p were shown as mean ± SEM. N = 3 experiments and N = 3 technical replicates were performed