Fig. 5

Expression of miR-15a-5p and miR-20b-5p in primary ECs following unassisted uptake of the miRNA mimics. ECs were cultured with elevated concentration (5, 25 and 100 nM) of mirVana miRNA mimic for A miR-15a-5p and C miR-20b-5p or their respective scrambled control. ECs were also cultured with identical concentration of LNA miRNA mimic for B miR-15a-5p and D miR-20b-5p, or their respective scrambled control. Following 48 h, total RNAs were isolated and expression levels miR-15a-5p, miR-20b-5p and reference miRNA miR-103a-3p were measured by qRT-PCR. Relative expression levels of miR-15a-5p and miR-20b-5p in comparison to miR-103a-3p were shown as mean ± SEM. N = 2 experiments and N ≥ 2 technical replicates were performed