Fig. 2

Polyfunctionality of CD4 T cell cytokine responses after GAG PTE POOL-2 stimulation. All possible combinations of positive responses are shown on the x-axis whereas frequencies of cytokine producing cells (absolute response) or percent of total CD4 T cell response (relative response) are shown on the y-axis of the scatter plots. The horizontal line shows the mean. Student’s t-test and Holm-Šídák correction for multiple comparisons test was used to compare the mean response for each CD4 T cell subset between HIV + SM+ (n = 13) and HIV + SM− (n = 14) responders. Only significant values are shown. To compare the pattern of representations of subsets of T cells, a partial permutation test was performed by Monte Carlo simulation with 10,000 iterations. A slice on a pie chart represents a Boolean gate defined subset of CD4 T cells secreting a single cytokine or a combination of cytokines. The size of a slice on the pie chart is the mean response from the scatter plot as a proportion of the overall response for each overlaid group. The colour of the squares at the bottom of the scatter plots matches that of the pie chart slices that correspond to each Boolean subset. The arcs around the pie charts indicate the production of individual cytokines over the Boolean subsets