Fig. 3

The effect of three different sera on the kinetics of proliferation of CIK cells. (A) The maximum expansion rate was seen in 10% hPL. (B) Low serum concentration dramatically reduced cell proliferation in the FBS group. (C) The maximum cell expansion rate occurred in all three groups at a concentration of 10% during the ninth and tenth days. Data analysis indicates that fold expansion and CPD of cultured cells in a medium containing hPL at three concentrations are significantly more than the FBS group at the end of culture. At the same time, there is no significant difference between FBS and HS groups. Also, in the PD/Day, maximum expansion was seen in time point 3 (TP3) in all groups at a concentration of 10%. The presented results are related to the differences between groups in TP10. (n = 3); These data were analyzed using Two-Way Repeated Measures ANOVA followed by Tukey test and shown as mean ± SEM. * as hPL compared with FBS group, # as hPL compared with HS group, and $ as HS compared with FBS group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001