Fig. 5

Viability of CIK cells in studied groups. (A) Cell viability at a concentration of 10% in all three sera is more than 90%. (B) Low serum concentration reduced cell viability in the FBS group. The data analysis showed that the viability percentage of cells cultured at different concentrations of hPL was significantly higher than the other two sera. In all three concentrations of hPL, CIK cells showed significantly higher viability than the other two groups (HS and FBS). Besides, HS in concentrations 5% and 2.5% exhibited significantly higher viability than FBS. No significant difference in viability was observed in different concentrations of hPL. The presented results are related to the differences between groups in TP10. (n = 3); These data were analyzed using Two-Way Repeated Measures ANOVA followed by Tukey test and shown as mean ± SEM. * as hPL compared with FBS group, # as hPL compared with HS group, and $ as HS compared with FBS group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001