Fig. 2

The distribution of TLR3 in different immune cells. WT mice were infected with S. japonicum, and livers were removed. Single-cell suspensions of liver were isolated from naive and infected mice. A Gate strategy for TLR3⁺ cells and CD4⁺T cells (CD4⁺CD3⁺), CD8⁺T cells (CD8⁺CD3⁺), γδT cells (γδTCR⁺CD3⁺), NKT cells (NK1.1⁺CD3⁺), B cells (CD19⁺CD3⁻), NK (NK1.1⁻CD3⁺) cells, MDSC (CD11b⁺Gr1⁺), macrophages (CD11b⁺F4/80⁺), DCs (CD11c⁺CD11b⁺) and neutrophils (CD11b⁺ Ly6g⁺) isolated from liver immune cells of WT and infected mice are shown. B The proportion and number of TLR3⁺ cells and CD4⁺T cells, CD8⁺ T cells, γδT cells, NKT cells, B cells NK cells, MDSC, macrophages, DCs and neutrophils from normal and infected mice are shown. Data were obtained from three independent experiments (n = 3–4) shown as the mean ± SD. *P < 0.05 and **P < 0.01