Fig. 1
From: PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6
PRMT2 is upregulated in LPS- or LPS/IFN-γ-treated BMDMs. (A) Quantitative polymerase chain reaction (qPCR) analysis of Prmts expression in BMDMs treated with LPS (1 µg/ml) for 6 h. (B) qPCR analysis of Prmt2 expression in BMDMs treated with LPS (1 µg/ml and 5 µg/ml) for 6 h. (C) qPCR analysis of Prmt2 expression in BMDMs treated with LPS (1 µg/ml) for the indicated times. (D-G) qPCR analysis of Tnf-α (D), Il-1β (E), and Nos2 (F) expression and Flow cytometry (FCM) analysis of CD86 (G) in BMDMs treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) for 24 h. FMO, fluorescence minus one. (H) qPCR analysis of Prmt2 expression in BMDMs treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) for 24 h. 18s rRNA was used as an endogenous reference for qPCR. Data are representative of three independent experiments (mean ± SD). ***p < 0.001, ****p < 0.0001