Fig. 3
From: PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6
PRMT2 silencing attenuates M2 polarization. (A-C) qPCR analysis of Il-10 (A) and Arg1 (B) expression and FCM analysis of CD206 (C) in BMDMs treated with IL-4 (20 ng/ml) for 24 h. (D) qPCR analysis of Prmt2 expression in BMDMs treated with IL-4 (20 ng/ml) for 24 h. (E-H) qPCR analysis of Il-10 (E) and Arg1(F) expression and FCM analysis of CD206 (G) in BMDMs transfected with Prmt2 siRNA for 48 h and then treated with IL-4 (20 ng/ml) for 24 h. (H) Quantification of mean fluorescence intensity of CD206 in (G). (I) Immunoblot analysis of ARG1 in BMDMs transfected with Prmt2 siRNA for 48 h and then treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) or IL-4 (20 ng/ml) for 24 h. 18s rRNA was used as an endogenous reference for qPCR. Data are representative of three independent experiments (mean ± SD). **p < 0.01, ****p < 0.0001