Fig. 4
From: PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6
PRMT2 silencing promotes M1 polarization through STAT1 activation. (A) Immunoblot analysis of phosphorylated STAT1, ERK, JNK, P38, and P65 or total proteins in lysates of BMDMs transfected with Prmt2 siRNA for 48 h and then treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) for the indicated times. (B-E) qPCR analysis of Tnf-α (B), Il-1β (C), and Nos2 (D) expression and FCM analysis of CD86 (E) in BMDMs transfected with Prmt2 siRNA for 48 h and then pretreated with STAT1 inhibitor Fludarabine (10 µM) for 1 h following by LPS (100 ng/ml) plus IFN-γ (20 ng/ml) stimulation for 24 h. (F) Quantification of mean fluorescence intensity of CD86 in (E). 18s rRNA was used as an endogenous reference for qPCR. Data are representative of three independent experiments (mean ± SD). *p < 0.05, ***p < 0.001, ****p < 0.0001