Fig. 5
From: PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6
PRMT2 silencing attenuates M2 polarization through STAT6 inhibition. (A) Immunoblot analysis of phosphorylated STAT3 and STAT6 or total proteins in lysates of BMDMs transfected with Prmt2 siRNA for 48 h and then treated with IL-4 (20 ng/ml) for the indicated times. (B-D) qPCR analysis of Il-10 (B) and Arg1 (C) expression and FCM analysis of CD206 (D) in BMDMs transfected with Prmt2 siRNA for 48 h and then pretreated with STAT6 inhibitor AS1517499 (1 µM) for 1 h following by IL-4 (20 ng/ml) stimulation for 24 h. (E) Quantification of mean fluorescence intensity of CD206 in (D). 18s rRNA was used as an endogenous reference for qPCR. Data are representative of three independent experiments (mean ± SD). **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant