Fig. 1

Cytometry-based gating strategies for leukoocytes’ subpopulations: T cells, B cells, monocytes, granulocytes and NK cells. A (1): Total lymphocytes’ population obtained based on SSC and FSC parameters. A (2): NK cells (CD3-CD56+) and NKT cells (CD3+CD56+) obtained by gating from A (1). A (3): CD4+ and CD8+ T cells obtained from CD3+CD56- T cells. B (1): Lymphocyte, monocyte and granulocyte populations were gated based on FSC-A and SSC-A in blood cells. B (2): Total B cells (CD19+) were gated from total lymphocytes using CD3 and CD19 monoclonal antibodies. B (3): Non-classical monocytes (CD16++CD14+), intermediate monocytes (CD14++CD16+), and classical monocytes (CD14++CD16-) were gated from total monocytes using CD14 and CD16 monoclonal antibodies. B (4): Neutrophil and eosinophil cells were identified based on CD16 in polymorphonuclear cells. SSC: Side scatter; FSC: Forward scatter; APC = allophycocyanin, PerCP = peridinin chlorophyll a protein; CD: Cluster of differentiation