Fig. 2

Gating strategy of BAT 1. Example of an analysis with the positive control anti-FcɛRI antibody. A Forward Scatter/Side Scatter plot was created to acquire the whole leukocyte population separated into three discrete populations (lymphocytes, monocytes and granulocytes). Plot 2 was created (CCR3-PE vs. Side Scatter) and basophils were gated as CCR3^pos and SSC^low. Plot 3 was created (CD63-FITC vs. CCR3-PE) to determine stimulated basophils. Activated basophils result in a CD63 positive basophil population (CD63^pos/CCR3^pos/SSC^low) identified in the upper right quadrant