Figure 3

Modulators of arginine metabolism restrain tumor growth. BALB/c (A) and C57BL/6 mice (B) were challenged s.c. with C26GM and RMA cells, respectively. The same day mice were randomly assigned to either one of the following treatments: L-NAME or sildenafil (given i.p. or dissolved in drinking water) or vehicle (PBS) i.p. (see Materials and Methods section for experimental details). Tumor volume (expressed as mm³) was measured by caliper at day 9 and 15, respectively. Panels report the cumulated data of at least three independent experiments performed with the following number of animals: C26GM model: Vehicle: n = 24, L-NAME i.p.: n = 15, L-NAME o.s.: n = 14, Sildenafil i.p.: n = 10, Sildenafil o.s.: n = 5; RMA model: Vehicle: n = 15, L-NAME o.s.: n = 15, Sildenafil o.s.: n = 10. Student's T test: ***p < 0.001, **0.001 < p < 0.05.