Figure 3

ME expression of genes related to TRIF signaling during OM, evaluated by microarray in WT mice. 3A. TRIF signaling genes. mRNA encoding genes of the TRIF signaling pathway (see Figure 1) in the ME mucosa. Data are expressed as fold induction over untreated ME mucosa (0 h). Each data point represents gene arrays obtained from 2 independent sets of 20 C57BL/6J mice each. 3B. Type I IFN genes. Not all IFNα genes are represented on the Affymetrix gene chip. However, data from those present are shown. View Within Article 3C. Type I IFN-induced genes. A number of genes are recognized as being induced by type I IFNs. Some of these genes also have ISREs in their promoters and can be directly activated by IRFs. A set of genes previously identified as being induced by IFNβ treatment in leukocytes [35] was assessed in the ME by gene array. (Detailed data including ranges and statistical significance are provided in Additional Files 1, 2 &3.)