Figure 3

Quantification of Tregs in the peripheral blood of recipient rats. The number of CD3+CD25+Foxp3+ Tregs cells in the blood of rats treated with RAPA on Day 1 (1D group)or Day 3 (3 D group) post-transplant were compared. Normal subjects (N) and heart transplant recipients without any immunosuppression were used as controls (Con group). No statistically significant difference between the groups was found among these three groups. Con group N: Normal spleen; POD 1: 1^st day after transplantation; POD 3: 3^rd days after transplantation; POD6: 6^th day after transplantation; 1 D group 1+1d: 1^st day after RAPA treatment in 1D group; 1+3d: 3^rd day after RAPA treatment in 1D group; 1+7d: 7^th day after RAPA treatment in 1D group; 1+10d: 10^th day after RAPA treatment in 1D group; 3D group 3+1d: 1^st day after RAPA treatment in 3D group; 3+3d: 3^rd day after RAPA treatment in 3D group; 3+7d: 7^th day after RAPA treatment in 3D group; 3+11d: 11^th day after RAPA treatment in 3D group.