Figure 3

Mannose receptor turnover in macrophage hybridoma cells. 43^MR cells (1.5 x 10⁷) were labeled with 1 μCi of ³⁵S -methionine overnight in methionine-free media. Following overnight incubation the cells were washed twice with PBS. Cells were then resuspended in complete media and plated into 6 well dishes. Cells were harvested at designated times and lysed in IP buffer in preparation for immunoprecipitation. Immunoprecipitates were resolved on a 7.5% gel and transferred to nitrocellulose. Relative pixel intensity of bands was determined by densitometry. Data represents the percent change in relative pixel intensity from the control at time 0. Results are representative of three independent experiments.