- Research article
- Open Access
Characterization of functional mannose receptor in a continuous hybridoma cell line
© Vigerust et al.; licensee BioMed Central Ltd. 2012
Received: 28 February 2012
Accepted: 30 August 2012
Published: 12 September 2012
The mannose receptor is the best described member of the type I transmembrane C-type lectins; however much remains unanswered about the biology of the receptor. One difficulty has been the inability to consistently express high levels of a functional full length mannose receptor cDNA in mammalian cells. Another difficulty has been the lack of a human macrophage cell line expressing a fully functional receptor. Commonly used human macrophage cell lines such as U937, THP-1, Mono-Mac and HL60 do not express the mannose receptor. We have developed a macrophage hybridoma cell line (43MR cells) created by fusion of U937 cells with primary human monocyte-derived macrophages, resulting in a non-adherent cell line expressing several properties of primary macrophages. The purpose of this study was to identify and select mannose receptor-expressing cells using fluorescence-activated cell sorting and to characterize the expression and function of the receptor.
In the current study we show that the mannose receptor found on this novel cell has endocytic characteristics consistent with and similar to the mannose receptor found on the surface of monocyte-derived human macrophages and rat bone marrow-derived macrophages. In addition, we demonstrate that these cells engage and internalize pathogen particles such as S. aureus and C. albicans. We further establish the transfectability of these cells via the introduction of a plasmid expressing influenza A hemagglutinin.
The 43MR cell line represents the first naturally expressed MR-positive cell line derived from a human macrophage background. This cell line provides an important cell model for other researchers for the study of human MR biology and host-pathogen interactions.
The mannose receptor (MR) is a 175 kDa type I transmembrane protein that was first described by Stahl and coworkers as a cell surface receptor involved in the clearance of extracellular hydrolases. Since that time many more roles have been ascribed to the MR including clearance of pathogens, capture of foreign antigens for presentation to MHC-II compartments[3, 4], clearance of glycoprotein hormones, clearance of extracellular peroxidases[6, 7], endocytosis of lysosomal acid phosphatase, and regulation of glycoprotein homeostasis. Recent work has suggested that the MR may serve as an entry receptor for several important human pathogens[10–14]. In addition to a cysteine-rich domain and fibronectin type II repeat, the MR structurally contains eight carbohydrate recognition domains (CRD), of which 4, 5 and 7 are reported to be the most critical for binding and internalization of ligands with exposed oligosaccharides terminating in mannose, fucose or N-acetylglucosamine.
A characteristic feature of the MR and other members of this family is their rapid internalization from the plasma membrane via a clathrin-mediated mechanism that delivers the receptors to the endocytic pathway[16, 17]. Several studies have shown that the MR binds and internalizes ligands via receptor-mediated endocytosis[18, 19], and participates in phagocytosis of mannosylated particles and pathogens[20, 21]. Mannosylated ligands bind to the MR at the cell surface at neutral pH and are brought into the cell, where they dissociate from the receptor in an acidic endosomal compartment[22, 23]. Ligands are then transported to the lysosome for degradation. Degraded particles are either packaged into MHC-II molecules or released into the extracellular media by exocytosis. It has been reported that 10-30% of the receptor at steady state resides on the cell surface and the remaining 70-90% is located in an intracellular pool. The MR has a long half-life (>30 hours), and makes 10 or more rounds of recycling each hour.
In addition to endocytic properties, several members of the MR family of molecules participate in phagocytosis, a function vital to the role of the macrophage in the innate immune response. Macrophages are found in virtually all tissues and are among the first cells to encounter an invading microorganism. The recognition capacity of the MR is broad allowing for the capture and uptake of a variety of pathogens including Paracoccidioides brasiliensis[26, 27]Candida albicans, Leishmania donovani, Mycobacterium tuberculosis[30, 31], Pneumocystis jirovecii (formally Pneumocystis carinii sp. hominis), Klebsiella pneumoniae, HIV[11, 34], Dengue, Hepatitis B, and influenza A[10, 37]. Mannose is not often found as a terminal residue on the cell surface of mammalian-derived proteins, but is commonly found on the surface of a variety of pathogens, thus allowing the MR to distinguish self from non-self through carbohydrate recognition.
Expression and function of the MR is tightly linked to the activation state of the macrophage and a variety of cytokines and chemical messengers are involved in the regulation of the MR. For example, interleukin (IL)-4, IL-10, IL-13 and prostaglandin E2 (PGE2) are known to up-regulate the expression of the MR[38–41], as does exposure to dexamethasone and vitamin D3[42–44]. Proinflammatory cytokines that activate macrophages at sites of inflammation such as interferon (IFN)-γ down-regulate MR expression. Exposure to pathogens such as Leishmania, C. albicans, bacillus Calmette-Guerin, HIV-1, and influenza likewise down-regulate receptor expression[46, 47]. This complex system of regulation is critical to the role that the MR plays in the resolution of inflammation, allowing for efficient removal of harmful extracellular enzymes such as myeloperoxidase, eosinophil peroxidases, tissue plasminogen activator, and lysosomal hydrolases. Further support for an in vivo role for the regulation of extracellular glycoproteins has come from studies with MR null mice that showed that the lack of MR results in decreased clearance of hydrolases and procollagens.
To date there are very few macrophage cell lines that express a functional MR. Commonly used human macrophage cell lines such as U937, THP-1, Mono-Mac and HL60 do not express the MR. Those cell lines that have been described as MR-positive are of murine or rat derivation[45, 48, 49]. In particular the MR-positive murine J774E and rat NR8383 cell lines have been extensively used in studies of MR function but until the current study there has been no available continuous human macrophage cell line that expresses the MR.
The MR has been implicated as a potential entry receptor for a variety of pathogens, and a target for regulation by human pathogen-associated proteins suggesting relevance for this receptor in the context of human disease[10, 11, 50]. Additionally, the glycosylation profile of many human pathogens makes uptake via the MR likely[37, 51–53]. In the current study we have characterized and cultivated a human hybridoma cell line that expresses MR. The utility of a human macrophage cell line and expression system for the MR will allow further study of MR biology and characterization of host-pathogen interactions between macrophages and causative agents of infectious disease. These tools will allow for further study of the biology and trafficking of the MR within the cell.
Macrophages used in this study included rat bone marrow-derived macrophages (RBMM), U937, and the continuous human macrophage hybridoma cell line 43 (obtained from Dr. K. Sperber, Mt. Sinai Medical Center), generated by fusing the hypoxanthine-guanosine phosphoribosyltranferase-deficient promonocytic cell line, U937, with human monocyte-derived macrophages (MDM). Fusion was documented by the acquisition of donor class I molecules. U937, 43 parental cells, and 43MR cells were maintained in RPMI 1640 with 10% FBS and antibiotics. RBMM were prepared from bone marrow obtained from Sprague–Dawley rats as previously described[55, 56] and maintained in RPMI with 10% FBS and antibiotics.
Plasmids and antibodies
The polyclonal antibody used in immunoblot analysis for the detection of MR was prepared in our laboratory. Monoclonal antibody against the human MR was obtained from BD Biosciences as an unlabeled antibody and also directly conjugated to Alexa 488 (San Jose CA). Mouse anti-hemagglutinin (HA) directly conjugated to Alexa 488 was obtained from Invitrogen (Carlsbad CA). Anti-EEA1 monoclonal antibody was obtained from BD Biosciences (San Jose CA). Antibodies conjugated to Alexa dyes (488, 555, and 568) for use in cytometry studies were obtained from Invitrogen (Carlsbad CA). Phycoerythrin (PE)-conjugated antibodies for use in fluorescent-activated cell sorting (FACS) were purchased from BD Biosciences (San Jose CA). Influenza PR8 HA plasmid was obtained from Adolfo Garcia-Sastre (Mt. Sinai Medical Center) and used for transfection assay to determine the capacity and efficiency of transfection.
To determine the efficiency of transfection, 43MR cells were transfected with Fugene (Roche) at a ratio of 6:1 according to manufacturer directions. Cells at 1 x 106 cells per well were plated into 6-well tissue culture dishes and transfected with 5 μg of PR8 HA plasmid. Cells were incubated at 37°C for 24 hours, and then harvested for flow cytometry as previously described.
Flow cytometry and confocal microscopy
Flow cytometry was performed using a FACSCaliber (BD Biosciences, San Jose CA) bench top analyzer in the VA and Vanderbilt cytometry core facilities. Cells were stained with PE-conjugated antibodies against MR as follows: cells were collected by centrifugation, followed by suspension in staining buffer (1% BSA, 0.1% sodium azide, 0.5% normal goat serum in PBS). 10% normal goat serum was included in the stain buffer to saturate Fc receptors and minimize non-specific fluorescence. PE-conjugated antibody was diluted in staining buffer and added to the cells. Additionally, an isotype matched control was also performed to account for non-specific fluorescence, and an unstained sample was included to account for autofluorescence. The cells were incubated for 20 minutes in the dark at 4°C. The cells were washed twice with staining buffer followed by fixation in 500μl of 2% buffered paraformalin. The modulation of cell surface receptor density was represented as the percent change in mean fluorescence intensity as compared to the control. The number of events acquired for each sample was 3 x 104 and cells were analyzed on a Becton Dickinson FACScan flow cytometer using CellQuest software or WinMDI FACS analysis software from J. Trotter (Scripps Research Institute, La Jolla CA). Characterization of the cell surface expression profile of the 43MR cells was performed by flow cytometry using antibody against typical macrophage surface expressed molecules (HLA ABC, CCR5, CD32, CD64, CD4, CD14, transferrin, DC-DSIGN, CXCR4, CD80, CD86, CD11) obtained from BD Biosciences (San Jose, CA). Confocal microscopy was performed using a Zeiss LSM-510 laser scanning inverted microscope. Cells were fixed in 2% buffered paraformalin followed by permeabilization with ice cold methanol. Following fixation and permeabilization, cells were stained in two ways. First, a two-step process using mouse monoclonal anti-MR antibody (BD Bioscience, San Jose CA) followed by either goat anti-mouse Alexa-488 or Alexa-568 (Invitrogen, Carlsbad CA) secondary. Second, a custom mouse monoclonal antibody against MR directly conjugated to Alexa 488 was also used. This second method eliminates the potential problem of non-specific secondary binding. Nuclei were stained for visualization with TO-PRO-3 (Invitrogen, Carlsbad CA). Cells were mounted in Aqua-polymount (Polysciences, Warrington PA) between coverslips. Images were collected at 520 nm using a 63x oil-immersion objective. Image processing was performed using Adobe Photoshop CS imaging software.
Phagocytosis was assayed via uptake of pHrodo BioParticles (Invitrogen, Eugene, OR). pHrodo S. aureus BioParticles conjugates are novel, no-wash fluorogenic particles used for quantitative measurement of phagocytosis. Since the fluorescent intensity of the bioparticle is tightly linked to the pH of the environment, particles in the extracellular environment typically display little to no fluorescence. The resulting fluorescence can therefore be attributed to internalized particles. Cells were cultured in complete media at a concentration of 5 x 105 in 6-well tissue culture dishes. After 24 hr, BioParticles prepared according to the manufacturer’s instructions were added to cells, and the plates incubated at 37°C for 30 minutes to allow for uptake. Cells were washed to remove unattached BioParticles, followed by fixation in 2% paraformalin and staining with anti-MR Alexa-488 conjugated antibody, and then visualized by confocal microscopy. In addition to examining the phagocytosis of labeled S. aureus, uptake of GFP-Candida was also employed. GFP-Candida was provided by L. Hoyer (U of Illinois) and incubated with cells in a 5:1 ratio at 37°C for 30 minutes. Samples were examined by confocal microscopy using anti-MR Alexa 568- or Alexa 555-conjugated antibody as described above.
Imunoprecipitation and immunoblot analysis
Cells (5 x 105) were lysed in lysis buffer containing 0.5% NP-40 or 0.1% Triton. Total cellular protein was determined either by bicinchoninic acid (BCA) protein assay (Pierce, Rockford IL) or by NanoDrop spectrophotometer (Thermoscientific) and 10-25 μg of total protein was resolved by electrophoresis on a 7.5 or 10% SDS-PAGE gel. Proteins were transferred to nitrocellulose followed by blocking in Tris-buffered saline (TBS) containing 0.1% Tween (TBST) and 5% powdered milk. Proteins were visualized on radiographic film using primary antibodies to the desired protein, horseradish peroxidase (HRP)-conjugated secondary antibodies, and chemiluminescence reagent. Immunoblot analysis was also performed on the Licor Odyssey platform using a goat anti-MR antibody followed by donkey anti-goat Ir-680 dye and a mouse anti-actin antibody followed by a donkey anti-mouse Ir-800 dye (Lincoln NE). For immunoprecipitation, cells were seeded into 6-well dishes at 5 x 105 cells per well and treated accordingly. Cells were lysed in 0.5% NP-40 lysis buffer and lysate was precleared with protein A-Sepharose overnight at 4°C followed by immunoprecipitation using rabbit polyclonal antibody against MR. The immunoprecipitated receptor was detected by immunoblot analysis after electrophoretic separation on 7.5% SDS-PAGE gel. Bands were visualized by chemiluminescence and exposure of Kodak Bio-MAX film. The density of the bands was quantified using the UN-SCAN IT densitometry software from Silk Scientific (Orem UT).
Receptor internalization was assessed using FITC-Man-BSA, FITC-BSA or fluorescent-labeled antibodies in the following manner: triplicate samples of 5 x 105 cells were pelleted in 12 x 75 mm Falcon tubes (Becton Dickinson Labware, Franklin Lakes NJ), washed twice in cold PBS to remove all media, and repelleted. The cells were placed on ice and a 1:100 dilution of anti-human MR conjugated to PE in cold staining buffer was used to resuspend the cells. Cells were incubated on ice for 15 min. Cells were washed twice in cold PBS and samples were taken to represent the total binding. Cells were then warmed to 37°C and triplicate samples were taken at 0, 10, 20, 30 minutes and placed on ice. Cells were pelleted and fixed in 500 μl cold 2% paraformalin containing 0.1% trypan blue to quench extracellular fluorescence. Cells were then analyzed by flow cytometry for total fluorescence. Fluorescence representing surface MR was plotted versus time to determine receptor internalization.
Uptake of horseradish peroxidase (HRP)
To measure ligand internalization, 10 μg of HRP was incubated with 43MR cells (5 x 105) in 400 μl Hanks’ balanced salt solution (HBSS) containing 1% BSA (binding buffer) as described previously[57, 58]. To control for non-specific uptake, yeast mannan (Sigma, St. Louis MO) was added at 1 mg/ml to companion wells as a competitor to HRP. Cells were incubated for 60 minutes at 37°C. Following incubations, the cells were washed twice in HBSS and solubilized in 250 μl of 0.1% Triton X-100. Cell-associated activity was determined by quantifying the oxidation of phenol red in the presence of hydrogen peroxide. HRP activity was then normalized to total cellular protein as measured by the BCA protein assay.
Binding of fluorescein isothyocyanate mannosylated-BSA (FITC-Man-BSA)
FITC-Man-BSA and FITC-BSA (20 μg) (E-Y laboratories, San Mateo CA) was incubated with 43MR cells (1 x 106) in 100 μl of staining buffer in the presence or absence of 1 mg/ml mannan. Incubations were done in triplicate and were incubated on ice for 30 minutes. Following incubation, cells were washed twice in cold staining buffer, fixed in 2% paraformalin and analyzed by flow cytometry.
Binding of 125 I-mannosylated-BSA binding (125I-Man-BSA)
Man-BSA was iodinated to a specific activity of 2 x 108 cpm/μg as previously described. Cells (5 x 105) were incubated with 125I-Man-BSA in 100 μl of binding buffer in the presence or absence of yeast mannan for 60 minutes on ice. Cell-associated counts were separated from unbound ligand via centrifugation through mineral oil, and cell-associated ligand was quantified by gamma counting.
Immunoprecipitation of 35S-labeled receptor
Cells were seeded into 6-well dishes at 5 x 105 cells per well and labeled by incubation with 100 μCi of 35S-methionine (Amersham, Piscataway NJ) in methionine-free DMEM overnight. Labeled receptor was immunoprecipitated using polyclonal anti-MR antibody as previously described. The immunoprecipitated receptor was analyzed on 7.5% SDS-PAGE gels. Bands were visualized by exposure of Kodak Bio-MAX film. The density of the bands was quantified using a scanner and UN-SCAN IT densitometry software.
Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of MR cDNA
RT-PCR of the 650 bp fragment was performed with a sense primer derived from rat and murine cDNA. The primer sequences were as follows: sense oligonucleotide (S3): 5’-GCTCTAGAATGGAACACACACTCTGGGCCATG-3’, antisense oligonucleotide (650AS): 5’-TACCACTTGTTTTCAAACTTGAA-3’. RT-PCR of the 300 bp cytoplasmic tail fragment (TMC) was performed with sense and antisense primers derived from the rat MR cDNA: sense oligonucleotide (TMCR): 5’-GAGACAGTCAGATTAGGATGGCTAA-3’ antisense oligonucleotide (TMCL): 5’-AAAGCTGACCAAAGGAAGATG-3’. PCR reaction conditions were as follows: total RNA was obtained from cell populations using Trizol and reverse transcribed using the First-Strand cDNA synthesis kit (Pharmacia, Piscataway, NJ). The PCR reaction mixture contained 1 μl of MR primer (100 ng/μl); 1 μl of a 20 mM dNTP solution; 5 μl of 100 mM Tris buffer with 15 mM MgCl2 and 500 mM KCl (pH. 8.3); 1 μl of template cDNA; 1 μl of Taq DNA polymerase (Promega); and 40 μl of diethlypyrocarbonate (DEPC) treated water. Thirty cycles of amplification were performed in a Hybaid OMN-E thermocycler (MR 650: 5 minutes at 92°C, 30 seconds at 92˚, 30 seconds at 48°C, and 1 minute at 68°C; MR TMC: 5 minutes at 95°C, 1 minute at 95°C, 1 minute at 55°C, and 1 minute at 72°C). Aliquots of the PCR reactions were then analyzed on agarose gels, and bands were visualized by ethidium bromide staining.
Statistics were performed using Prism 5 (GraphPad Software). For experiments involving two comparisons, a paired Student’s two-tailed t-test was used. For experiments involving in excess of two comparisons, a one way ANOVA with post-test was performed. Significance was determined by p values less than 0.05. All experiments presented are representative of at least 3 determinations with error bars that represent the standard deviation unless otherwise noted in the figure legends.
Selection of MR-positive 43 cells
Cell surface expression characteristics of the 43MR macrophage hybridoma cell line
Cell surface expression profile of macrophages and macrophage cell lines
HLA DR DQ
Expression of the MR protein by 43MR Cells
Reverse transcriptase polymerase chain reaction (RT-PCR)
Expression of MR mRNA was assessed by RT-PCR using oligonucleotides derived from the rat MR cDNA sequence. The primers used generated an internal 650 bp fragment within the extracellular region of the MR and a 300 bp fragment from the C-terminal end of the cytoplasmic tail of the MR. Figure 2C demonstrates that both a 650 bp and 300 bp fragment was seen in the 43MR cells, consistent with previously published data from RBMM, and was not detected in parental 43 cells (data not shown).
Mannose receptor turnover in 43MR cells
Binding and uptake of ligands mediated by the MR in 43MR cells
The MR participates in the internalization of a variety of glycosylated proteins. HRP is a known ligand for the MR and has been used extensively to examine the endocytic capacity of the MR. To determine the endocytic activity of the MR in 43MR cells, uptake of HRP was measured in and compared to the uptake in RBMM, U937 and the 43neg parental cell line in the presence and absence of mannan. As shown in Figure 4B, 43MR cells efficiently ingested HRP, with uptake of ~50% of RBMM activity. The MR negative cells 43neg and U937 did not internalize a significant amount of HRP. These results indicate that the MR in 43MR cells can mediate uptake of traditional ligands.
Several investigators have adopted the use of flow cytometry to determine the endocytic capacity of membrane-associated receptors. Results can be obtained by using either tagged ligands or tagged antibodies raised against the receptor. We used a FITC-labeled ligand specific (Man-BSA) for the MR to determine the level of uptake exhibited by 43MR cells in the presence of absence of same specific competitor (Man). The fluorometric approach has greater sensitivity than the HRP approach and is further improved by having a ligand with higher avidity (Man-BSA) than HRP. Our results indicate that approximately 60% of cells specifically internalized FITC-Man-BSA (Figure 5). Control experiments using FITC conjugated to a non-mannosylated BSA was also performed to demonstrate that the FITC-BSA ligand itself is not internalized by these cells. Therefore, this uptake was specific for the MR as evidenced by the reduction in internalization in the presence of excess mannan (Figure 5).
Phagocytic activity of 43MR
Macrophages are critical members of the innate immune system, and play a pivotal role in the clearance of pathogens, foreign particles and endogenous ligands. One of these receptors is the MR which recognizes extracellular host and foreign substances with exposed terminal mannose residues. Through this binding, the MR mediates the internalization of a wide range of pathogens and host-derived molecules; participates in the resolution of inflammation; and mediates clearance of apoptotic cells, endogenous hydrolases, and peroxidases[6, 57, 68]. In this study we have demonstrated that 43MR macrophage hybridoma cells express the 175 kDa MR. This MR appears to function similarly to the receptor in primary macrophages and is regulated by agents known to affect the expression of the MR[39, 69, 70]. To our knowledge this is the first description of a continuous human macrophage cell line that expresses a functional MR.
Several continuous monocyte and macrophages lines currently exist that have been used to study macrophage biology including HL60, U937, THP-1, and MonoMac cells[71–74]. The cell surface marker profile of these cell types varies substantially and none express the exact complement of surface markers that are seen on primary macrophage. One disadvantage of using these cell types as surrogates for primary macrophages is that none express the MR, and this lack of an appropriate cell line has limited the study of MR biology in human systems. Much of the work that has been carried out has been performed in rat or murine macrophage cell lines that may or may not represent an accurate picture of the biology of the receptor in humans[45, 49, 75]. The parental 43 hybridoma cell line was described by Sperber et al. and was created by fusion of the U937 macrophage continuous cell line with primary monocyte-derived macrophages. The resultant cells expressed donor-derived MHC I molecules and several other macrophage cell surface markers, but did not express detectable levels of the MR. In the current study, cells were grown to high confluence and cell sorting was used to select a subpopulation of cells expressing very low levels of MR. After successive sorts we ultimately obtained a population of cells in which >95% of the cells were MR-positive, and capable of being passaged with high MR maintenance. The isolation and characterization of these cells is significant for extending the characterization of the MR to human systems.
In the current study several experimental methods were employed to demonstrate that the MR in 43MR cells is equivalent to the 175 kDa MR found in primary human and rodent macrophages. First, using PCR products were amplified from both the N- and C-terminal regions of the MR, indicating that these cells express functional domains important in ligand binding and receptor-ligand trafficking. Second, in immunoblot analysis using polyclonal anti-MR antibodies, a 175 kDa MR was observed as previously reported for the rat and human MR[49, 76]. These findings are consistent with observations suggesting that concentrations of MR in rodent macrophages can be as much as 10 times greater than those found in human. Third, confocal imaging demonstrated a receptor expressed on the surface and in cytoplasmic compartments that is consistent with previous descriptions of MR localization. In addition to immunoblot analysis illustrating a receptor of the same molecular weight and cellular distribution seen by confocal analysis, data from the surface binding and uptake assays showed ligand binding and internalization equivalent to results previously reported for primary human macrophages.
Another well-documented property of the MR is the correlation of expression with the functional state of the macrophage. Previous studies have shown that agents and cytokines such as dexamethasone, IL-4 and GM-CSF that promote a mature “deactivated” macrophage phenotype result in the induction of MR expression and altered MR. For example, Cowan et al. reported that treatment with dexamethasone increased MR expression on rodent macrophages by as much as 2.5 fold in a time and dose-dependent fashion. Chakraborty et al. reported that dexamethasone-induced increases in the MR resulted in increased uptake of Leishmania parasites, and Zhu et al. demonstrated that dexamethasone treatment increased clearance of glucocerebrocidase by the MR. In data not presented, we examined the effect of positive regulators (dexamethasone, GM-CSF, and IL-4) on MR expression in 43MR cells. Dexamethasone at 0.1 μg/ml increased MR expression to approximately 130% of control, slightly under the previously reported increase seen in rat macrophages (150-200%:[42, 69]). These results suggest that the MR in 43MR cells can be positively regulated by dexamethasone as previously reported, and therefore provide further evidence that the MR expressed by 43MR cells is functionally similar to the MR found in primary human macrophages.
The macrophage MR plays a key role in a number of innate host defense activities, including mediating entry of a variety of important human pathogens and clearance of harmful extracellular hydrolases. In the current study we demonstrated that two particle ligands previously shown to be internalized via the MR - C. albicans and the Gram positive bacteria S. aureus – were internalized by 43MR cells, suggesting that the MR on these cells functions as a phagocytic receptor for these organisms.
Finally, we demonstrate the capacity of these cells to express exogenous proteins via transient transfection. In this study and in others we have successfully transiently expressed viral proteins into these cells using several different transfection preparations. The capacity for transient transfection allows for the introduction of proteins to study MR trafficking and its interaction with pathogen proteins.
Understanding the molecular and cell biology of this receptor could be key to developing strategies for containing infection and controlling inflammation. However, lack of appropriate human cellular models that are available in large numbers and easily transfectable has hampered progress in this area. In the current study we have described a new human macrophage cell line developed by fusion of a human monocytic cell line (U937) and human monocyte-derived macrophages that express a functional MR on its surface, binds traditional MR ligands, and internalizes these ligands appropriately. In addition these cells can be transfected to a high efficiency, and are capable of expressing high levels of exogenous DNA. These cells therefore provide a powerful tool for the study of MR biology with respect to its role in innate immunity, and the role of the MR and the macrophage in the pathogenesis of disease.
This work was supported by grants from the Department of Veteran Affairs (VLS, DJV). Research support for DJV was also provided in part through a National Heart, Lung and Blood Institute Training Grant T32-HL07751. Confocal imaging and analysis were performed in part through the use of the Vanderbilt University Medical Center Cell Imaging Core Resource (Supported by NIH Grants CA68485, DK20593, and DK58404).
- Stahl P, Six H, Rodman JS, Schlesinger P, Tulsiani DR, Touster O: Evidence for specific recognition sites mediating clearance of lysosomal enzymes in vivo. Proc Natl Acad Sci U S A. 1976, 73 (11): 4045-4049. 10.1073/pnas.73.11.4045.PubMedPubMed CentralGoogle Scholar
- Pontow SE, Kery V, Stahl PD: Mannose receptor. Int Rev Cytol. 1992, 137B: 221-244.PubMedGoogle Scholar
- Engering AJ, Cella M, Fluitsma D, Brockhaus M, Hoefsmit EC, Lanzavecchia A, Pieters J: The mannose receptor functions as a high capacity and broad specificity antigen receptor in human dendritic cells. Eur J Immunol. 1997, 27 (9): 2417-2425. 10.1002/eji.1830270941.PubMedGoogle Scholar
- Prigozy TI, Sieling PA, Clemens D, Stewart PL, Behar SM, Porcelli SA, Brenner MB, Modlin RL, Kronenberg M: The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. Immunity. 1997, 6 (2): 187-197. 10.1016/S1074-7613(00)80425-2.PubMedGoogle Scholar
- Simpson DZ, Hitchen PG, Elmhirst EL, Taylor ME: Multiple interactions between pituitary hormones and the mannose receptor. Biochem J. 1999, 343 (Pt 2): 403-411.PubMedPubMed CentralGoogle Scholar
- Shepherd VL, Hoidal JR: Clearance of neutrophil-derived myeloperoxidase by the macrophage mannose receptor. Am J Respir Cell Mol Biol. 1990, 2 (4): 335-340.PubMedGoogle Scholar
- Lang T, de Chastellier C, Frehel C, Hellio R, Metezeau P: Leao Sde S, Antoine JC: Distribution of MHC class I and of MHC class II molecules in macrophages infected with Leishmania amazonensis. J Cell Sci. 1994, 107 (Pt 1): 69-82.PubMedGoogle Scholar
- Imai K, Yoshimura T: Endocytosis of lysosomal acid phosphatase; involvement of mannose receptor and effect of lectins. Biochem Mol Biol Int. 1994, 33 (6): 1201-1206.PubMedGoogle Scholar
- Lee SJ, Evers S, Roeder D, Parlow AF, Risteli J, Risteli L, Lee YC, Feizi T, Langen H, Nussenzweig MC: Mannose receptor-mediated regulation of serum glycoprotein homeostasis. Science. 2002, 295 (5561): 1898-1901. 10.1126/science.1069540.PubMedGoogle Scholar
- Reading PC, Miller JL, Anders EM: Involvement of the mannose receptor in infection of macrophages by influenza virus. J Virol. 2000, 74 (11): 5190-5197. 10.1128/JVI.74.11.5190-5197.2000.PubMedPubMed CentralGoogle Scholar
- Nguyen DG, Hildreth JE: Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages. Eur J Immunol. 2003, 33 (2): 483-493. 10.1002/immu.200310024.PubMedGoogle Scholar
- Vigerust DJ, Egan BS, Shepherd VL: HIV-1 Nef mediates post-translational down-regulation and redistribution of the mannose receptor. J Leukoc Biol. 2005, 77 (4): 522-534.PubMedGoogle Scholar
- Faure M, Rabourdin-Combe C: Innate immunity modulation in virus entry. Current opinion in virology. 2011, 1 (1): 6-12. 10.1016/j.coviro.2011.05.013.PubMedGoogle Scholar
- Fanibunda SE, Modi DN, Gokral JS, Bandivdekar AH: HIV gp120 binds to mannose receptor on vaginal epithelial cells and induces production of matrix metalloproteinases. PLoS One. 2011, 6 (11): e28014-10.1371/journal.pone.0028014.PubMedPubMed CentralGoogle Scholar
- Taylor ME, Bezouska K, Drickamer K: Contribution to ligand binding by multiple carbohydrate-recognition domains in the macrophage mannose receptor. J Biol Chem. 1992, 267 (3): 1719-1726.PubMedGoogle Scholar
- Zvaritch E, Lambeau G, Lazdunski M: Endocytic properties of the M-type 180-kDa receptor for secretory phospholipases A2. J Biol Chem. 1996, 271 (1): 250-257. 10.1074/jbc.271.1.250.PubMedGoogle Scholar
- Harding C, Levy MA, Stahl P: Morphological analysis of ligand uptake and processing: the role of multivesicular endosomes and CURL in receptor-ligand processing. Eur J Cell Biol. 1985, 36 (2): 230-238.PubMedGoogle Scholar
- Stahl PD, Rodman JS, Miller MJ, Schlesinger PH: Evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages. Proc Natl Acad Sci U S A. 1978, 75 (3): 1399-1403. 10.1073/pnas.75.3.1399.PubMedPubMed CentralGoogle Scholar
- Stahl P, Schlesinger PH, Sigardson E, Rodman JS, Lee YC: Receptor-mediated pinocytosis of mannose glycoconjugates by macrophages: characterization and evidence for receptor recycling. Cell. 1980, 19 (1): 207-215. 10.1016/0092-8674(80)90402-X.PubMedGoogle Scholar
- Linehan SA, Martinez-Pomares L, da Silva RP, Gordon S: Endogenous ligands of carbohydrate recognition domains of the mannose receptor in murine macrophages, endothelial cells and secretory cells; potential relevance to inflammation and immunity. Eur J Immunol. 2001, 31 (6): 1857-1866. 10.1002/1521-4141(200106)31:6<1857::AID-IMMU1857>3.0.CO;2-D.PubMedGoogle Scholar
- Kang BK, Schlesinger LS: Characterization of mannose receptor-dependent phagocytosis mediated by Mycobacterium tuberculosis lipoarabinomannan. Infect Immun. 1998, 66 (6): 2769-2777.PubMedPubMed CentralGoogle Scholar
- Wileman T, Harding C, Stahl P: Receptor-mediated endocytosis. Biochem J. 1985, 232 (1): 1-14.PubMedPubMed CentralGoogle Scholar
- Wileman T, Boshans R, Stahl P: Uptake and transport of mannosylated ligands by alveolar macrophages. Studies on ATP-dependent receptor-ligand dissociation. J Biol Chem. 1985, 260 (12): 7387-7393.PubMedGoogle Scholar
- Diment S, Stahl P: Macrophage endosomes contain proteases which degrade endocytosed protein ligands. J Biol Chem. 1985, 260 (28): 15311-15317.PubMedGoogle Scholar
- Wileman T, Boshans RL, Schlesinger P, Stahl P: Monensin inhibits recycling of macrophage mannose-glycoprotein receptors and ligand delivery to lysosomes. Biochem J. 1984, 220 (3): 665-675.PubMedPubMed CentralGoogle Scholar
- Tavares AH, Derengowski LS, Ferreira KS, Silva SS, Macedo C, Bocca AL, Passos GA, Almeida SR, Silva-Pereira I: Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. PLoS Negl Trop Dis. 2012, 6 (1): e1459-10.1371/journal.pntd.0001459.PubMedPubMed CentralGoogle Scholar
- Nakaira-Takahagi E, Golim MA, Bannwart CF, Puccia R, Peracoli MT: Interactions between TLR2, TLR4, and mannose receptors with gp43 from Paracoccidioides brasiliensis induce cytokine production by human monocytes. Medical mycology: official publication of the International Society for Human and Animal Mycology. 2011, 49 (7): 694-703.Google Scholar
- Porcaro I, Vidal M, Jouvert S, Stahl PD, Giaimis J: Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages. J Leukoc Biol. 2003, 74 (2): 206-215. 10.1189/jlb.1202608.PubMedGoogle Scholar
- Chakraborty P, Ghosh D, Basu MK: Modulation of macrophage mannose receptor affects the uptake of virulent and avirulent Leishmania donovani promastigotes. J Parasitol. 2001, 87 (5): 1023-1027.PubMedGoogle Scholar
- Li YJ, Petrofsky M, Bermudez LE: Mycobacterium tuberculosis uptake by recipient host macrophages is influenced by environmental conditions in the granuloma of the infectious individual and is associated with impaired production of interleukin-12 and tumor necrosis factor alpha. Infect Immun. 2002, 70 (11): 6223-6230. 10.1128/IAI.70.11.6223-6230.2002.PubMedPubMed CentralGoogle Scholar
- Schlesinger LS, Kaufman TM, Iyer S, Hull SR, Marchiando LK: Differences in mannose receptor-mediated uptake of lipoarabinomannan from virulent and attenuated strains of Mycobacterium tuberculosis by human macrophages. J Immunol. 1996, 157 (10): 4568-4575.PubMedGoogle Scholar
- Ezekowitz RA, Williams DJ, Koziel H, Armstrong MY, Warner A, Richards FF, Rose RM: Uptake of Pneumocystis carinii mediated by the macrophage mannose receptor. Nature. 1991, 351 (6322): 155-158. 10.1038/351155a0.PubMedGoogle Scholar
- Athamna A, Ofek I, Keisari Y, Markowitz S, Dutton GG, Sharon N: Lectinophagocytosis of encapsulated Klebsiella pneumoniae mediated by surface lectins of guinea pig alveolar macrophages and human monocyte-derived macrophages. Infect Immun. 1991, 59 (5): 1673-1682.PubMedPubMed CentralGoogle Scholar
- Liu Y, Liu H, Kim BO, Gattone VH, Li J, Nath A, Blum J, He JJ: CD4-independent infection of astrocytes by human immunodeficiency virus type 1: requirement for the human mannose receptor. J Virol. 2004, 78 (8): 4120-4133. 10.1128/JVI.78.8.4120-4133.2004.PubMedPubMed CentralGoogle Scholar
- Miller JL, de Wet BJ, Martinez-Pomares L, Radcliffe CM, Dwek RA, Rudd PM, Gordon S: The mannose receptor mediates dengue virus infection of macrophages. PLoS Pathog. 2008, 4 (2): e17-10.1371/journal.ppat.0040017.PubMedPubMed CentralGoogle Scholar
- Op den Brouw ML, Binda RS, Geijtenbeek TB, Janssen HL, Woltman AM: The mannose receptor acts as hepatitis B virus surface antigen receptor mediating interaction with intrahepatic dendritic cells. Virology. 2009, 393 (1): 84-90. 10.1016/j.virol.2009.07.015.PubMedGoogle Scholar
- Tate MD, Brooks AG, Reading PC: Specific sites of N-linked glycosylation on the hemagglutinin of H1N1 subtype influenza A virus determine sensitivity to inhibitors of the innate immune system and virulence in mice. J Immunol. 2011, 187 (4): 1884-1894. 10.4049/jimmunol.1100295.PubMedGoogle Scholar
- Martinez-Pomares L, Reid DM, Brown GD, Taylor PR, Stillion RJ, Linehan SA, Zamze S, Gordon S, Wong SY: Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies. J Leukoc Biol. 2003, 73 (5): 604-613. 10.1189/jlb.0902450.PubMedGoogle Scholar
- Egan BS, Abdolrasulnia R, Shepherd VL: IL-4 modulates transcriptional control of the mannose receptor in mouse FSDC dendritic cells. Arch Biochem Biophys. 2004, 428 (2): 119-130. 10.1016/j.abb.2004.04.019.PubMedGoogle Scholar
- Coste A, Dubourdeau M, Linas MD, Cassaing S, Lepert JC, Balard P, Chalmeton S, Bernad J, Orfila C, Seguela JP: PPARgamma promotes mannose receptor gene expression in murine macrophages and contributes to the induction of this receptor by IL-13. Immunity. 2003, 19 (3): 329-339. 10.1016/S1074-7613(03)00229-2.PubMedGoogle Scholar
- DeFife KM, Jenney CR, McNally AK, Colton E, Anderson JM: Interleukin-13 induces human monocyte/macrophage fusion and macrophage mannose receptor expression. J Immunol. 1997, 158 (7): 3385-3390.PubMedGoogle Scholar
- Shepherd VL, Konish MG, Stahl P: Dexamethasone increases expression of mannose receptors and decreases extracellular lysosomal enzyme accumulation in macrophages. J Biol Chem. 1985, 260 (1): 160-164.PubMedGoogle Scholar
- Clohisy DR, Bar-Shavit Z, Chappel JC, Teitelbaum SL: 1,25-Dihydroxyvitamin D3 modulates bone marrow macrophage precursor proliferation and differentiation. Up-regulation of the mannose receptor. J Biol Chem. 1987, 262 (33): 15922-15929.PubMedGoogle Scholar
- Piemonti L, Monti P, Allavena P, Leone BE, Caputo A, Di Carlo V: Glucocorticoids increase the endocytic activity of human dendritic cells. Int Immunol. 1999, 11 (9): 1519-1526. 10.1093/intimm/11.9.1519.PubMedGoogle Scholar
- Harris N, Super M, Rits M, Chang G, Ezekowitz RA: Characterization of the murine macrophage mannose receptor: demonstration that the downregulation of receptor expression mediated by interferon-gamma occurs at the level of transcription. Blood. 1992, 80 (9): 2363-2373.PubMedGoogle Scholar
- Shepherd VL, Lane KB, Abdolrasulnia R: Ingestion of Candida albicans down-regulates mannose receptor expression on rat macrophages. Arch Biochem Biophys. 1997, 344 (2): 350-356. 10.1006/abbi.1997.0219.PubMedGoogle Scholar
- Ezekowitz RA, Austyn J, Stahl PD, Gordon S: Surface properties of bacillus Calmette-Guerin-activated mouse macrophages. Reduced expression of mannose-specific endocytosis, Fc receptors, and antigen F4/80 accompanies induction of Ia. J Exp Med. 1981, 154 (1): 60-76. 10.1084/jem.154.1.60.PubMedGoogle Scholar
- Helmke RJ, Boyd RL, German VF, Mangos JA: From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell Dev Biol. 1987, 23 (8): 567-574. 10.1007/BF02620974.PubMedGoogle Scholar
- Lane KB, Egan B, Vick S, Abdolrasulnia R, Shepherd VL: Characterization of a rat alveolar macrophage cell line that expresses a functional mannose receptor. J Leukoc Biol. 1998, 64 (3): 345-350.PubMedGoogle Scholar
- Schlesinger LS: Macrophage phagocytosis of virulent but not attenuated strains of Mycobacterium tuberculosis is mediated by mannose receptors in addition to complement receptors. J Immunol. 1993, 150 (7): 2920-2930.PubMedGoogle Scholar
- Vigerust DJ, Ulett KB, Boyd KL, Madsen J, Hawgood S, McCullers JA: N-Linked glycosylation attenuates H3N2 influenza viruses. J Virol. 2007.Google Scholar
- Vigerust DJ, Shepherd VL: Virus glycosylation: role in virulence and immune interactions. Trends Microbiol. 2007, 15 (5): 211-218. 10.1016/j.tim.2007.03.003.PubMedGoogle Scholar
- Lee RT, Hsu TL, Huang SK, Hsieh SL, Wong CH, Lee YC: Survey of immune-related, mannose/fucose-binding C-type lectin receptors reveals widely divergent sugar-binding specificities. Glycobiology. 2011, 21 (4): 512-520. 10.1093/glycob/cwq193.PubMedPubMed CentralGoogle Scholar
- Sperber K, Bauer J, Pizzimenti A, Najfeld V, Mayer L: Identification of subpopulations of human macrophages through the generation of human macrophage hybridomas. J Immunol Methods. 1990, 129 (1): 31-40. 10.1016/0022-1759(90)90417-T.PubMedGoogle Scholar
- Weikert LF, Edwards K, Chroneos ZC, Hager C, Hoffman L, Shepherd VL: SP-A enhances uptake of bacillus Calmette-Guerin by macrophages through a specific SP-A receptor. Am J Physiol. 1997, 272 (5 Pt 1): L989-L995.PubMedGoogle Scholar
- Boltz-Nitulescu G, Wiltschke C, Holzinger C, Fellinger A, Scheiner O, Gessl A, Forster O: Differentiation of rat bone marrow cells into macrophages under the influence of mouse L929 cell supernatant. J Leukoc Biol. 1987, 41 (1): 83-91.PubMedGoogle Scholar
- Sung SS, Nelson RS, Silverstein SC: The role of the mannose/N-acetylglucosamine receptor in the pinocytosis of horseradish peroxidase by mouse peritoneal macrophages. J Cell Physiol. 1983, 116 (1): 21-25. 10.1002/jcp.1041160105.PubMedGoogle Scholar
- Rabinovitch M, Topper G, Cristello P, Rich A: Receptor-mediated entry of peroxidases into the parasitophorous vacuoles of macrophages infected with Leishmania Mexicana amazonensis. J Leukoc Biol. 1985, 37 (3): 247-261.PubMedGoogle Scholar
- Shepherd VL, Abdolrasulnia R, Garrett M, Cowan HB: Down-regulation of mannose receptor activity in macrophages after treatment with lipopolysaccharide and phorbol esters. J Immunol. 1990, 145 (5): 1530-1536.PubMedGoogle Scholar
- Aldridge GM, Podrebarac DM, Greenough WT, Weiler IJ: The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J Neurosci Methods. 2008, 172 (2): 250-254. 10.1016/j.jneumeth.2008.05.003.PubMedPubMed CentralGoogle Scholar
- Shepherd VL, Campbell EJ, Senior RM, Stahl PD: Characterization of the mannose/fucose receptor on human mononuclear phagocytes. J Reticuloendothel Soc. 1982, 32 (6): 423-431.PubMedGoogle Scholar
- Astarie-Dequeker C, N'Diaye EN, Le Cabec V, Rittig MG, Prandi J, Maridonneau-Parini I: The mannose receptor mediates uptake of pathogenic and nonpathogenic mycobacteria and bypasses bactericidal responses in human macrophages. Infect Immun. 1999, 67 (2): 469-477.PubMedPubMed CentralGoogle Scholar
- Bodmer JL, Dean RT: Receptor-mediated phagocytosis of zymosan is unaffected by some conditions which reduce macrophage lysosomal enzyme secretion. Biosci Rep. 1983, 3 (11): 1053-1061. 10.1007/BF01121032.PubMedGoogle Scholar
- Bozeman PM, Hoidal JR, Shepherd VL: Oxidant-mediated inhibition of ligand uptake by the macrophage mannose receptor. J Biol Chem. 1988, 263 (3): 1240-1247.PubMedGoogle Scholar
- East L, Isacke CM: The mannose receptor family. Biochim Biophys Acta. 2002, 1572 (2–3): 364-386.PubMedGoogle Scholar
- Sklar LA, Edwards BS, Graves SW, Nolan JP, Prossnitz ER: Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annu Rev Biophys Biomol Struct. 2002, 31: 97-119. 10.1146/annurev.biophys.31.082901.134406.PubMedGoogle Scholar
- Le Cabec V, Emorine LJ, Toesca I, Cougoule C, Maridonneau-Parini I: The human macrophage mannose receptor is not a professional phagocytic receptor. J Leukoc Biol. 2005, 77 (6): 934-943. 10.1189/jlb.1204705.PubMedGoogle Scholar
- Lang T, de Chastellier C: Fluid phase and mannose receptor-mediated uptake of horseradish peroxidase in mouse bone marrow-derived macrophages. Biochemical and ultrastructural study. Biology of the cell/under the auspices of the European Cell Biology Organization. 1985, 53 (2): 149-154. 10.1111/j.1768-322X.1985.tb00362.x.PubMedGoogle Scholar
- Cowan HB, Vick S, Conary JT, Shepherd VL: Dexamethasone up-regulates mannose receptor activity by increasing mRNA levels. Arch Biochem Biophys. 1992, 296 (1): 314-320. 10.1016/0003-9861(92)90578-K.PubMedGoogle Scholar
- Stein M, Keshav S, Harris N, Gordon S: Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation. J Exp Med. 1992, 176 (1): 287-292. 10.1084/jem.176.1.287.PubMedGoogle Scholar
- Engel A, Chatterjee SK, Al-arifi A, Riemann D, Langner J, Nuhn P: Influence of spacer length on interaction of mannosylated liposomes with human phagocytic cells. Pharm Res. 2003, 20 (1): 51-57. 10.1023/A:1022294624256.PubMedGoogle Scholar
- Roche AC, Midoux P, Pimpaneau V, Negre E, Mayer R, Monsigny M: Endocytosis mediated by monocyte and macrophage membrane lectins–application to antiviral drug targeting. Res Virol. 1990, 141 (2): 243-249. 10.1016/0923-2516(90)90028-H.PubMedGoogle Scholar
- Ding J, Umstead TM, Floros J, Phelps DS: Factors affecting SP-A-mediated phagocytosis in human monocytic cell lines. Respir Med. 2004, 98 (7): 637-650. 10.1016/j.rmed.2003.12.018.PubMedGoogle Scholar
- Suzuki T, Ohno N, Ohshima Y, Yadomae T: Soluble mannan and beta-glucan inhibit the uptake of Malassezia furfur by human monocytic cell line, THP-1. FEMS Immunol Med Microbiol. 1998, 21 (3): 223-230.PubMedGoogle Scholar
- Mukhopadhyay A, Stahl P: Bee venom phospholipase A2 is recognized by the macrophage mannose receptor. Arch Biochem Biophys. 1995, 324 (1): 78-84. 10.1006/abbi.1995.9926.PubMedGoogle Scholar
- Stahl PD, Ezekowitz RA: The mannose receptor is a pattern recognition receptor involved in host defense. Curr Opin Immunol. 1998, 10 (1): 50-55. 10.1016/S0952-7915(98)80031-9.PubMedGoogle Scholar
- Rossi G, Himmelhoch S: Immunocytochemical localization of the mannose receptor on ultrathin cryosections of chicken macrophages. Eur J Cell Biol. 1985, 38 (2): 280-291.PubMedGoogle Scholar
- Zhu Y, Li X, Schuchman EH, Desnick RJ, Cheng SH: Dexamethasone-mediated up-regulation of the mannose receptor improves the delivery of recombinant glucocerebrosidase to Gaucher macrophages. J Pharmacol Exp Ther. 2004, 308 (2): 705-711.PubMedGoogle Scholar
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