Figure 7
From: Characterization of functional mannose receptor in a continuous hybridoma cell line
Transient transfection of influenza A hemagglutinin in 43MRcells. 43MR cells (1 x 106) were seeded in 6-well culture dishes and transfected with 5 μg of Influenza A PR8 HA expressing plasmid. Cells were then incubated for 24 hours at 37. Following incubation, the cells were harvested and stained for flow cytometry with Alexa 488 conjugated anti-HA (red line). Cells were also harvested for unstained (shaded gray) and transfection media alone control samples (blue line). The number of events acquired for each sample was 3 x 104. Data shown are the mean fluorescence intensity as compared to the unstained and transfection media alone control.