- Open Access
Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi
© Acevedo et al; licensee BioMed Central Ltd. 2013
Published: 25 February 2013
The proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by a detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by the i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce a higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by the parenteral route with the Cuban anti-typhoid vaccine vax-TyVi®, although this vaccine did not induce a mucosal response. In conclusion, this work demonstrates that PLc can be used as a mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.
Enteric infections induced by pathogens are one of the main causes of death all over the world . Vibrio cholerae kills more than 100 000-130 000 persons each year, most of them children and adolescents between 5 and 19 years old. Current vaccines are mainly based on attenuated or inactivated whole bacterial cells . Alternatively, our group has been working on the development of a non-living adjuvant/delivery strategy based on PLc, a proteoliposome-like structure extracted from the strain C7258 of V. cholerae O1 El Tor Ogawa . PLc contain important antigens and immunostimulatory molecules like lipopolysaccharides (LPS), OmpU porin and MSHA. Recent work demonstrated that PLc induced a higher IgA response in mucosal fluids as well as IgG in sera against V. cholerae antigens; furthermore the vibriocidal activity of sera of mice immunized with PLc  encouraged our group to further study the potential immunoadjuvant properties of this structure. Proteoliposomes derived from bacteria have also been used as an adjuvant to potentiate the immune response against heterologous antigens , particularly a Neisseria derived proteosome has been used to increase the immunogenicity of LPS from Shigella flexneri . Therefore we thought to evaluate the adjuvant potential of PLc for the capsular polysaccharide Vi of S. Typhi (Poli Vi). Typhoid fever caused by invasion of S. Typhi through enteric mucosa constitutes also a health problem; every year more than 500 000 persons die and most of them are children about 5- 19 years old from developing countries . Strategies based on attenuated whole cell vaccines or purified Poli Vi vaccines have not been able to prevent Typhoid fever especially in infants .
Materials and methods
Antigens and vaccines. PLc was obtained from the V. cholerae C7258 strain using a detergent protocol of extraction, reported by Pérez JL et al. . The structural characterization was carried out using electron microscopy, photon correlation spectroscopy and zeta potential analysis. PLc composition was evaluated by Lowry protein assay and western blot (MSHA, LPS and OmpU) as later described . Poli Vi (lot 8003) administered alone or coadministered with PLc and the vaccine vax-TyVi® (lot 9009) were supplied by Finlay Institute, Havana, Cuba.
Immunization and sample collection
Female BALB/c mice (6-8 weeks old, CENPALAB, Cuba) were immunized with a three-dose schedule, 7 days apart (0, 7, 14). Each dose of 20 μL contained 100 μg of PLc plus Poli Vi 25 μg and was administered to mice by i.n route without anesthesia (10 μL per nostril). Control groups with Poli Vi alone were administered with the same schedule by the i.n route (25 µg per dose per mouse) or one intramuscular (i.m) dose of vax-TyVi® (5 µg per dose per mouse). Placebo control groups were immunized with PBS buffer. Samples were collected 7 days (saliva and faeces) or 14 days (sera) after the last dose as described elsewhere . Animals were housed at the Finlay Institute animal facility. The experiment was performed by duplicate and with approval of the Finlay Institute Ethical Committee.
Determination of antibodies by ELISA
IgG anti-Poli Vi antibodies in serum samples and IgA anti-Poli Vi antibodies in saliva and faeces were measured by indirect ELISA as described elsewhere . Briefly, PoliSorp plates (Nunc, Roskilde, Denmark) were set 1 h at room temperature (20-25°C) with poli-L-Lysine at 3 μg/mL (100 μL per well) and then coated with Poli Vi (100 μL per well) at 5 μg/mL. Serum samples were diluted 1:100 and saliva and faeces 1:2 (in PBS supplemented with 1% (w/v) BSA). Peroxidase anti-mouse IgG, or anti mouse IgA were used as secondary antibodies. O-phenylene diamine (OPD) was used as the substrate to detect antibodies bound to the antigen. Optical densities were read at 492 nm using an ELISA reader (Titertek Multiskan®).
Analysis of data was carried out using ANOVA. Post test multiple comparison was performed with Graph Pad Prism 4 software (CA, USA).
PLc extraction and characterization
Effect of coadministration of PLc and Poli Vi
Mucosal surfaces constitute one of the most important points of entry of infectious agents; protection against infections requires the induction of immune responses at mucosal level . Polysaccharides and LPS from enteric pathogens are considered as some of the most important antigens to induce protection . However, their mucosal administration has proven to be inefficient for the stimulation of the immune system . Mucosal adjuvants can overcome this problem and potentiate their immune responses . Protollin is a complex mix of outer membrane proteins from Neisseria meningitidis B and LPS from Shig uella flexneri (proteosome), this formulation was administered by the i.n route to humans and mice and improved the immune response against LPS antigen [4, 10]. Therefore, we evaluated the effect of coadministration of PLc with Poli Vi by the i.n route.
Results showed that proteoliposomes extracted from V. cholerae O1 (Figure 1A) contain immunogenic and immunostimulatory molecules like OmpU, MSHA and LPS (Fig 1B). Coadministration of PLc with the polysaccharide improves the mucosal and systemic immune response induced vs. Poli Vi (Figure 2) without affecting the immune response vs V. cholerae antigens (results not shown). Purified Poli Vi antigen has been used in the formulation of a parenteral vaccine against typhoid fever  but has never been used by a mucosal route as a with vaccine purposes. Mucosal immunization has been approached by administration by the oral route of the attenuated S. Typhi strain CVD 909 (HolaVax-Typhoid®) expressing the Vi polysaccharide . In these experiments the systemic immune response was superior to that induced by the attenuated S. Typhi strain Ty21a which do not express Poli Vi . Ty21a is also the strain used in the licensed vaccine Vivotif®, against typhoid fever . Specific IgG anti Poli Vi in sera is crucial to avoid pathogen invasion to macrophages and evasion from immunocompetent cells . The role of IgA anti Poli Vi may also be related to the blockade of pathogen invasion at a mucosal level , however the mechanisms of the protection induced by our formulation needs to be evaluated.
The use of mucosal vaccines has many advantages when compared to parenteral administration. Particularly, if vaccine formulations are intended against pathogens that colonize or invade via the mucosal route, like V. cholerae and S. Typhi . Mucosal immunisation offers an attractive two-pronged approach as it stimulates both local and systemic immunity . Furthermore, it also offers the potential for rapid administration in mass vaccination programs  without risk of injury and cross-infection through contaminated needles . In addition, with a growing awareness for environmental impact, disposal of needles, decontamination costs, and incineration waste products has helped to influence development of mucosal vaccines as a greener alternative .
Overall, PLc has been demonstrated to be immunogenic against V. cholerae O1  and also has immune adjuvant effects when coadministered with Poli Vi. PLc is part of the adjuvant family developed by the Finlay Institute, AFPL (Adjuvant Finlay Proteoliposome) and named AFPL2. Further studies are envisaged to evaluate the adjuvant potential of AFPL2 with other antigens and using other mucosal routes like oral to develop a multiple mucosal vaccine candidate against enteric pathogens.
This research was supported by Finlay Institute and the Research and Development Fund from Strathclyde University. We would like to thank to Dr. Gustavo Falero for kindly providing access to monoclonal antibodies against V. cholerae antigens.
This article has been published as part of BMC Immunology Volume 14 Supplement 1, 2013: Proceedings from Delivery Systems and Current strategies to drug design. The full contents of the supplement are available online at http://www.biomedcentral.com/bmcimmunol/supplements/14/S1.
- State of the art of new vaccine research and development. [http://www.who.int/vaccine_research/documents/stateoftheart/en/index.html]
- Pérez JL, Acevedo R, Callicó A, Fernández Y, Cedré B, Año G, González L, Falero G, Talavera A, Pérez O: A proteoliposome based formulation administered by the nasal route produces vibriocidal antibodies against El Tor Ogawa Vibrio cholerae O1 in balb/c mice. Vaccine. 2009, 25: 205-212.View ArticleGoogle Scholar
- Pérez O, Lastre M, Cabrera O, del Campo J, Bracho G, Cuello M, Acevedo R: New Vaccines Require Potent Adjuvants like AFPL1 and AFCo1. Scandinavian Journal of Immunology. 2007, 66: 271-277. 10.1111/j.1365-3083.2007.01981.x.View ArticlePubMedGoogle Scholar
- Orr N, Arnon R, Rubin G, Cohen D, Bercovier H, Lowell GH: Enhancement of anti-Shigella lipopolysaccharide (LPS) response by addition of the cholera toxin B subunit to oral and intranasal proteosome-Shigella flexneri 2a LPS vaccines. Infect Immun. 1994, 62 (11): 5198-5200.PubMed CentralPubMedGoogle Scholar
- Acevedo R, Callicó A, del Campo J, González E, Cedré B, González L, Romeu B, Zayas C, Lastre M, Fernández S: Intranasal administration of proteoliposome-derived cochleates from Vibrio cholerae O1 induce mucosal and systemic immune responses in mice. Methods. 2009, 49 (4): 309-315. 10.1016/j.ymeth.2009.03.027.View ArticlePubMedGoogle Scholar
- Ramirez JC, Serrano B, Lara M, Farinas M, Mirabal M, Sifontes S, Garcia I, Gonzales P, Lopes Y, Garcia A: Study of immunogenicity in mice of the Cuban Vi Polysaccharide Typhoid vaccine, vax-TyVi. VacciMonitor. 2006, 15 (2): 1-4.Google Scholar
- Yuki Y, Kiyono H: Mucosal Vaccines: novel advances in technology and delivery. Expert Rev Vaccines. 2009, 8 (8): 1083-1097. 10.1586/erv.09.61.View ArticlePubMedGoogle Scholar
- Dietrich G, Griot-Wenk M, Metcalfe IC, Lang AB, Viret JF: Experience with registered mucosal vaccines. Vaccine. 2003, 21: 678-683. 10.1016/S0264-410X(02)00579-0.View ArticlePubMedGoogle Scholar
- Leroux-Roels G: Unmet needs in modern vaccinology: adjuvants to improve the immune response. Vaccine. 2010, 28S: 25-36.View ArticleGoogle Scholar
- Fries LF, Montemarano AD, Mallett CP, Taylor DN, Hale TL, Lowell GH: Safety and immunogenicity of a proteosome-Shigella Flexneri 2a lipopolysaccharide vaccine administered intranasally to healthy adults. Infect Immun. 2001, 69 (7): 4545-4553. 10.1128/IAI.69.7.4545-4553.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Guzman CA, Borsutzky S, Griot-Wenk M, Metcalfe IC, Pearman J, Collioud A, Favre D, D G: Vaccines against typhoid fever. Vaccine. 2006, 24: 3804-3811. 10.1016/j.vaccine.2005.07.111.View ArticlePubMedGoogle Scholar
- Garmory HS, Brown KA, Titball RW: Salmonella vaccines for use in humans: present and future perspectives. FEMS Microbiology Reviews. 2002, 26: 339-353.PubMedGoogle Scholar
- Raffatellu M, Chessa D, Wilson RP, Tukel C, Akcelik M, Baumler AJ: Capsule-Mediated Immune Evasion: a New Hypothesis Explaining Aspects of Typhoid Fever Pathogenesis. Infect Immu. 2006, 74 (1): 19-27. 10.1128/IAI.74.1.19-27.2006.View ArticleGoogle Scholar
- Giudice EL, Campbell JD: Needle-free vaccine delivery. Adv Drug Deliver Rev. 2006, 58 (1): 68-89. 10.1016/j.addr.2005.12.003.View ArticleGoogle Scholar
- Brody S: Declining HIV rates in Uganda: due to cleaner needles, not abstinence or condoms. Int J STD AIDS. 2004, 15: 440-441. 10.1258/0956462041211324.View ArticlePubMedGoogle Scholar
- Report of the third meeting of the Steering Committee on Immunization Safety. [http://www.who.int/vaccines-documents/DocsPDF03/www746.pdf]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.