Recent attempts to diminish nickel use in most industrial products have led to an increasing utilization of alternative metal compounds for destinations such as the alloys used in orthopaedics, jewellery and dentistry. The present study was undertaken with the aim to evaluate the potential for an allergic response to nickel, palladium and rhodium on the basis of antigen-specific induction of inflammatory/regulatory cytokines, and to characterize, according to the cytokine profiles, the nature of simultaneous positive patch tests elicited in vivo.
Peripheral blood mononuclear cells (PBMC) from 40 patients with different patch test results were kept in short term cultures in the presence of optimized concentrations of NiSO4 × 6H2O, PdCl2 and Rh(CH3COO)2. The production of IFN-γ and IL-10 elicited by metal compounds were analyzed by the ELISpot assay.
Results
We found a specific IFN-γ response by PBMC upon in vitro stimulation with nickel or palladium in well recognized allergic individuals. All controls with a negative patch test to a metal salt showed an in vitro IL-10 response and not IFN-γ production when challenged with the same compound. Interestingly, all subjects with positive patch test to both nickel and palladium (group 3) showed an in vitro response characterized by the release of IFN-γ after nickel stimulation and production of IL-10 in response to palladium.
Conclusion
These results strongly suggest that the different cytokine profiles elicited in vitro reflect different immune responses which may lead to the control of the allergic responses or to symptomatic allergic contact dermatitis. The development of sensitive and specific in vitro assays based on the determination of the cytokine profiles in response to contact allergens may have important diagnostic and prognostic implications and may prove extremely useful in complementing the diagnostic limits of traditional patch testing.
Background
Allergic contact dermatitis (ACD) affects a large fraction of the general population being most frequently elicited by nickel (Ni), which is responsible of a delayed-type hypersensitivity clinically revealed by the demonstration of eczematous reactions upon patch testing and by laboratory testing of antigen-specific T-cell proliferation [1–3]. These alterations are the result of the interaction between the metal ions, which act as haptens, and the skin surface, thus favouring the contact and binding with cellular and extracellular matrix proteins [4–6], and the induction of a cellular immune response against the hapten-carrier protein complex [7, 8]. Recent attempts to diminish Ni use in most industrial products have led to an increase in the use of different metal compounds for specific destinations such as the alloys used in orthopaedics, jewellery and dentistry [9]. For instance, the use of palladium (Pd) increased in the past 10 years [10] also due to the fact that dentistry alloys containing Pd appear more resistant to mechanical wearing effects [11, 12]. Similarly, rhodium (Rh), which is primarily used as an alloying agent to harden platinum and Pd, it is now employed in electrical material because of its low electrical and contact resistance, in jewellery (platings of silver or white gold because of its silvery white colour), in optical instruments and dental prostheses.
Nevertheless, it has been recently recognized that also exposure to metal ions such as Pd, chromium, cobalt and gold, can induce a cellular immune response and clinical manifestations of ACD, even if less frequently than Ni [13–15]. In fact, positive patch testing to PdCl2 and clinical manifestation of ACD to Pd have been described with increasing frequency [16, 17]. Despite its low sensitization potential, also Rh has been reported as sensitizer in the form of salt [18].
In Ni sensitized subjects a simultaneous patch test reactivity to Pd has been frequently reported [19, 20] and a double sensitization can be hypothesised especially in individuals wearing dental prosthesis. Nevertheless, a cutaneous cross-reactivity cannot be excluded for the chemical and structural similarities of Ni and Pd [21]. Metal ions are incomplete antigens which have to bind to endogenous peptides to be "visible" to the immune system [7] and Ni and Pd encountering the same cellular environment might form in vivo complexes with similar proteins thus eliciting cross-recognizing immune responses [17]. Moreover, two independent in vitro studies demonstrated an immunologic cross reactivity between these metals at the T cell clonal level [22, 23].
Identifying both a double sensitization and a lymphocyte cross-reactivity to different metal ions in vivo is an important issue for clinicians in directing the choice of implant materials in order to avoid incompatible reactions. Consequently, the diagnosis of ACD to both Ni and Pd needs in vitro assays complementary to patch testing [24, 25]. Indeed, the variability of the readouts obtained with patch testing have stimulated a number of studies aimed at developing assays characterizing the immune response to metal salts. Recently, Minang et al. [26] analyzed the different cytokine profiles induced by nickel in allergic or tolerant subjects by enzyme-linked immunospot (ELISpot) assay. In fact, the sensitivity and specificity of the ELISpot assay allow the measurement of very low frequencies of cytokine-producing cell, therefore offering a powerful tool for the detection and characterization of Ni sensitization with important implications for diagnostic strategies [27, 28].
The present study was undertaken with the aim to evaluate the potential of the ELISpot assay in determining sensitivities to Ni or Pd and to Ni and Pd on the basis of specific induction of inflammatory/regulatory cytokines. Since all subjects were skin challenged to Ni, Pd and Rh salts, and none of them had positive skin reactivity to Rh, this metal was employed to assess the cytokine profile in non allergic subjects and to explore the possibility to identify a cellular immunity against materials with traces of heavy metals.
Results
Patch tests
To analyse the cytokine response to metals, we considered four different groups of subjects according to their skin responses. As summarized in Table 1, group 1 included 10 patients with a strong positive reaction to Ni; group 2 included 10 patients with strong (n = 7) or moderate (n = 3) hypersensitivity to Pd; group 3 included 10 patients showing a strong reactivity to Ni and a simultaneous weak (n = 4) or moderate (n = 6) response to Pd; and, finally, group 4 included 10 patients with negative patch testing to all metals investigated. All patients enrolled in the study (n = 40) had a negative patch test response to Rh.
Table 1
Characteristics of patients and healthy controls.
Group Subject n.
Sex
Age range (median)
Patch test to Ni
Patch test to Pd
Patch test to Rh
1 n = 10
9 F
28 – 40 (30)
9 +++
9 -
9 -
1 M
30
1 +++
1 -
1 -
2 n = 10
9 F
28 – 38 (33)
9 -
7+++/2 ++
9 -
1 M
40
1 -
1 ++
1 -
3 n = 10
9 F
29 – 40 (32)
9 +++
6 ++/3 +
9 -
1 M
39
1+++
1 +
1 -
4 n = 10
9 F
28 – 38 (33)
9 -
9 -
9 -
1 M
36
1 -
1 -
1 -
As described in the methods, patch test reactivity to the different metal salts was defined as strong (+++: oedema, erythema, papules and vesicles), moderate (++: oedema, erythema and papules), weak (+: oedema and erythema) or no reaction (-).
IFN-γ and IL-10 production by PBMC in response to Ni, Pd and Rh
To determine the production of IFN-γ and IL-10 upon in vitro stimulation, PBMC from patients with different patch test results were kept in short term cultures in the presence of NiSO4 × 6H2O, PdCl2 and Rh(CH3COO)2 and analyzed by the ELISpot assay.
IFN-γ and IL-10 basal response or elicited by metal salts or PHA in PBMC from different groups of patients are shown in Figure 1. The primary readouts of spot forming cells for each experimental condition in individual patients are also shown in Table 2.
Figure 1
Cytokine response elicited by metals or PHA by ELISpot analysis. Elispot results of detection of IFN-γ or IL-10 producing PBMC upon stimulation with Ni (20 μg/ml), Pd (2.5 μg/ml), Rh (5 μg/ml) or PHA (1 μg/ml) were expressed as Index values. Index is expressed by the ratio between the number of spot forming cells (IFN-γ and IL-10 producing PBMC) upon stimulation with metal salts and those present in the absence of stimuli (spontaneous cytokine production). The mean values of Index values ± SD were obtained for the 4 different groups of 10 subjects according to their in vivo reactivity to metal salts. Cytokine producing cells in control triplicate wells with medium alone was the baseline value (Index = 1). Panel A: patch test positive to Ni (group 1); B: patch test positive to Pd (group 2); C: patch test positive to Ni and Pd (group 3); D: negative patch test (group 4). The statistical analysis between IFN-g and IL-10 producing cells upon stimulation with Ni or Pd between patients belonging to group C and A or B was performed using the Mann-Whitney test. The highly significant differences (P < 0.0001), found between group C and B, are indicated by symbols (*, **, °, °°).
Table 2
Individual values of spot forming cells for each experimental condition expressed as mean values and Standard Errors of triplicate wells.
IFN-γ
IL-10
Subject n.
Mean
Std. Error
W/O
PHA
Ni
Pd
Rh
W/O
PHA
Ni
Pd
Rh
Patch test positive to Ni
1
Mean
18,67
151
116
14,33
5,667
19
132
36
79,67
76
(Group 1)
Std. Error
2,404
4,359
3,055
1,202
0,8819
0,5774
3,606
2,082
3,93
3,786
2
Mean
25,17
164,3
150
29,33
17,67
21
157,3
36,5
73
86,33
Std. Error
1,74
6,766
3,215
2,333
1,453
2,082
6,36
0,2887
2,309
2,333
3
Mean
27,67
203
136,5
19,33
16,67
11,67
92
22,5
47
52
Std. Error
2,333
3,606
0,866
0,8819
1,453
0,8819
3,606
1,443
3,606
2,887
4
Mean
26,67
203
168,3
18,67
5,333
33,33
270
62,67
51,33
132,7
Std. Error
2,404
9,074
3,48
1,202
1,202
1,202
6,351
6,333
1,667
5,207
5
Mean
30,67
246,7
168,3
27,33
27,33
38
289
65,33
143,3
147,7
Std. Error
0,8819
1,856
2,603
0,8819
1,764
2,517
6,083
2,603
2,963
7,311
6
Mean
13,33
100,3
53
25
6,667
22
177
32,67
79,33
93,67
Std. Error
2,186
3,18
3,055
1,732
1,202
5,568
6,658
2,906
0,8819
4,667
7
Mean
21,33
171,7
119,3
10,83
10,5
21,67
147,3
35,67
85,33
91
Std. Error
0,3333
3,667
4,667
0,6009
0,2887
2,848
5,925
3,283
2,603
2,082
8
Mean
20
158
129,3
10,33
15,5
35
265,7
46,5
136
132,7
Std. Error
1,155
3,464
3,18
1,764
1,443
2,309
11,98
0,866
6,245
5,487
9
Mean
20,67
160,7
118,3
4,333
8,333
33,5
233
59
129,3
130
Std. Error
1,764
5,239
4,41
1,453
1,453
0,866
7,506
2,887
5,783
3,215
10
Mean
13,33
105,3
65
14,67
2,667
15,5
107,7
25,33
59
62
Std. Error
2,404
5,696
2,082
1,453
1,202
1,607
3,844
3,844
2,082
1,732
Patch test positive to Pd
11
Mean
10
69,33
11
45,33
2,333
27
197,3
84,33
21
129,3
(Group 2)
Std. Error
1,155
3,48
0,5774
2,603
1,333
2,887
7,219
2,404
3,606
3,18
12
Mean
14,33
100,3
20,67
83,67
15,67
16,5
124,7
58,67
10,33
68,33
Std. Error
3,844
1,453
2,333
4,631
1,202
1,443
3,283
1,202
2,603
2,906
13
Mean
13
92
19,33
62,33
14
37,67
285
143
38
186
Std. Error
3,215
5,686
2,028
3,844
1,528
1,453
5,508
2,309
1,732
3,464
14
Mean
13,5
101
17
71,67
10,67
35,5
285
127,7
30
151
Std. Error
0,866
7,937
3,215
2,333
0,8819
0,2887
5,508
4,41
1,155
1,528
15
Mean
45
360,3
41
229,7
36,33
9,333
73,67
37,67
10,67
41,33
Std. Error
1,732
9,351
1,732
2,906
3,48
1,764
2,728
1,202
1,764
1,856
16
Mean
6,5
53
5,667
34
2
12
92
47,33
13,67
45,33
Std. Error
1,443
2,082
1,202
1,155
1,528
1,528
2
1,856
1,764
1,764
17
Mean
15,67
117,3
9,667
72,67
6,333
23,33
190
84
22
93
Std. Error
3,48
7,688
0,6667
1,453
0,8819
2,848
2,082
2,082
2,309
1,155
18
Mean
6,667
52
5,333
32
2
28,67
198,3
110,3
24
112
Std. Error
1,764
3,512
0,8819
2,082
1
1,856
10,4
9,171
1,155
7,371
19
Mean
32,67
273
36
170,3
13,33
10,33
70,33
34,33
9,667
44
Std. Error
1,202
3,606
2,646
5,548
1,202
0,8819
2,728
3,283
1,202
2,646
20
Mean
30,67
244
24,33
153,3
7
28,67
197,3
120,7
31,67
116
Std. Error
1,202
4,509
2,028
2,848
1,528
2,404
2,333
8,95
1,764
4,041
Patch test positive to Ni and Pd
21
Mean
14
105,7
89,67
17,67
10,67
20,33
153,7
32,67
72,33
81
(Group 3)
Std. Error
2,646
8,293
6,692
2,404
1,764
1,453
5,783
3,528
2,333
3,786
22
Mean
24,83
172
150
25
25
22
176
26,67
79,67
93,67
Std. Error
2,048
3,786
3,464
2,082
2,887
5,568
6,658
3,283
2,333
2,603
23
Mean
26,67
203,7
157
37,33
14,67
38,67
292,3
66,67
148,7
152
Std. Error
1,453
8,686
4,619
2,333
1,764
2,186
5,783
1,856
4,055
3,606
24
Mean
31
247
186
30,67
18,33
23,67
186,3
21,33
94,67
119,3
Std. Error
1,155
2,309
3,464
1,453
2,028
4,702
7,623
3,528
4,41
1,856
25
Mean
25,67
197
157,7
22,33
18
37,33
285
49
145,7
143
Std. Error
1,764
4,041
1,856
2,028
2,309
1,453
5,508
3,215
3,383
3,215
26
Mean
20
157,7
117
16
10
15,5
125,3
29,67
56
61,67
Std. Error
1,155
3,18
2,082
1,155
2,517
0,2887
4,667
1,667
2,887
1,764
27
Mean
12,33
95,67
70,33
12
9,667
30,33
210,7
39,67
114,7
118,3
Std. Error
1,453
10,37
3,383
1,528
2,028
3,18
6,984
4,978
3,48
3,756
28
Mean
45
361
244,3
49,67
52,67
31,67
215
50
124,7
133
Std. Error
1,732
9,292
4,096
3,283
2,333
2,963
5,132
2,646
3,48
2,309
29
Mean
45,5
373,3
285
60
40
38,33
264,3
72,67
161,7
153,3
Std. Error
2,021
2,963
6,429
1,732
3,512
4,055
12,25
2,028
4,055
3,48
30
Mean
15,67
118
96
6,333
16,33
33,67
254,3
54
132
131,3
Std. Error
2,333
3,215
6,928
2,028
2,728
2,963
8,413
2,309
5,292
2,963
Patch test negative
31
Mean
3,667
24
6
4,667
4,667
32,33
243
146,7
116,7
134,3
(Group 4)
Std. Error
0,8819
1,528
1,155
0,8819
1,202
2,028
4,041
4,631
6,173
5,608
32
Mean
17,33
130
27,67
21,33
33
47,33
377
237,3
181
201,3
Std. Error
2,333
2,887
1,764
3,283
1,732
2,728
2,646
2,603
3,215
6,064
33
Mean
6,667
53,33
10,33
4,333
5,667
18,33
140,3
69,67
66,33
90,67
Std. Error
1,202
2,728
0,8819
1,453
0,6667
1,453
2,603
2,404
4,842
4,631
34
Mean
11,33
82,67
15
8
8
24,33
193,3
98
99
108
Std. Error
0,8819
2,333
2,082
1,732
1,528
1,202
3,844
1,732
4,619
5,196
35
Mean
14,33
112,7
17,33
18,67
7,333
34,33
260,3
134
111,7
132
Std. Error
1,202
5,044
1,453
2,028
0,8819
2,404
3,18
1,528
4,41
4,359
36
Mean
3
23
3,333
0,6667
2,333
17
138
78,33
83
67,67
Std. Error
0,5774
1,528
1,453
0,6667
0,3333
2,646
4,163
1,453
3,055
2,404
37
Mean
18
141
23,33
7,333
4,333
15
109,7
57,33
57,33
58,67
Std. Error
1,732
5,033
1,453
1,202
0,8819
1,732
2,906
2,404
3,18
4,256
38
Mean
22,67
180,7
27,33
18,33
11,67
29
196,7
154,3
114,3
122,7
Std. Error
1,202
1,202
2,404
1,453
1,202
1,732
1,453
2,404
6,009
4,41
39
Mean
6,667
54
6,333
2
5,333
10
71,33
52,67
40,33
40,33
Std. Error
1,202
2
0,8819
1,155
1,856
1,155
2,028
2,028
2,906
4,096
40
Mean
13,33
105,3
10,67
11
15
27,67
199,3
146,3
107,7
107
Std. Error
1,453
3,712
0,8819
1,528
1,528
1,453
3,756
11,26
4,978
7
Patients were grouped following their patch test reactions. W/O: medium alone; PHA: polyclonal stimulation; Ni 20 μg/ml, Pd 2.5 μg/ml, Rh 5 μg/ml.
PHA stimulated cultures invariably showed increased numbers of IFN-γ or IL-10 producing cells with Indexes ranging from 6.8 to 8.3, and the values did not significantly differ among the different groups, therefore excluding differences in the capability of different subjects to rise a specific cytokine response or impairment of cellular function due to cryopreservation.
Remarkably, IFN-γ releasing cells upon metal stimulation were the most represented in strict association with results of the in vivo responses. In fact, IFN-γ-Ni responsive cells (Index > 3) were detected in subjects with positive patch test to Ni either alone (group1) or in association with moderate or weak response to Pd (group 3) (P = 0.28). IFN-γ-Pd responsive cells were detected only in subjects with strong or moderate Pd positive patch testing (group 2), and not in subjects with moderate or weak in vivo response to Pd associated with a strong Ni reactivity (group 3) (P < 0.0001).
All subjects with negative patch test results showed an in vitro IL-10 production upon metal stimulation while any positive or ambiguous IFN-γ response to Ni, Pd or Rh was present. Interestingly a different in vitro behaviour was found between subjects showing a monosensitization to Pd and subjects with concomitant skin reactivity to Pd and Ni. In fact, these subjects (group 3) had an in vitro reactivity more similar to Ni monosensitized patients (IFN-γ-Ni responsive cells: P = 0.28; IL-10-Ni responsive cells: P = 0.19) rather than to Pd monosensitized subjects (IFN-γ-Ni responsive cells P < 0.0001; IL-10-Ni responsive cells: P < 0.0001).
Overall results showed that IL-10 producing cells in response to metals were invariably associated to a lack of IFN-γ production. This is well visualized by the scatter plots (Figure 2) of individual Index values obtained in response to Ni or Pd in all groups of patients, where it can be appreciated that IFN-γ or IL-10 production in response to metal salts are mutually exclusive, while PHA is capable of inducing both cytokines. Statistical analysis confirmed the significant negative correlation between IFN-γ and IL-10 production (P < 0.0001). The same diagram (Figure 2) reporting the individual responses in the different group of patients also shows that the in vivo moderate and weak reactivity to Pd, observed in association to strong reactivity to Ni (group 3), did not match the in vitro response, that was clearly sustained by IL-10 rather than IFN-γ producing cells.
Figure 2
Scatter plot analysis of individual IFN-γ and IL-10 induction by PHA, Ni or Pd in the different group of patients. PHA induces PBMC release of both IFN-γ and IL-10 in all subjects, while metal salts induces IFN-γ or IL-10 production according to the in vivo positive or negative response. IFN-γ or IL-10 production was alternatively produced and consequently a highly significant negative correlation was found (Spearman analysis P < 0.0001). Panel A: IFN-γ and IL-10 induction (Index) by PHA or Ni salt; B: IFN-γ and IL-10 induction (Index) by PHA or Pd salt. Red Diamond: Group 1 (induced by Ni in panel A or by Pd in panel B), Green diamond: Group 2 (induced by Ni in panel A or by Pd in panel B), Blue square: Group 3 (induced by Ni in panel A or by Pd in panel B), Grey circle: Group 4 (induced by Ni in panel A or by Pd in panel B), Yellow triangle: Overall subjects (induced by PHA).
Discussion
In the present study, we have investigated the profiles of cytokine production by PBMC of ACD patients and healthy controls in response to the well known contact allergens Ni and Pd, or to Rh, considered to have a low sensitization potential, through the ELISpot assay.
The results showed that PBMC from well recognized allergic individuals affected by ACD have a higher frequency of IFN-γ releasing cells upon in vitro stimulation with Ni or Pd, as compared to control groups. Most interestingly, we found that PBMC from all the individuals exhibiting a negative skin response to a metal salt (group 4) revealed a clear IL-10 response when challenged with the same metal, in the absence of any detectable IFN-γ response. Accordingly, all subjects included in the study had a negative skin response to Rh, which was invariably associated to Rh-induced IL-10 production in vitro.
The immunologic mechanisms responsible for the clinical hypersensitivity towards metals have been recently reviewed [7]. It is known that Ni-specific, IFN-γ-producing CD4+ and CD8+ T cells are major effectors of sensitization and directly responsible for skin manifestations [6, 29], whereas IL-10 and TGF-β-secreting regulatory T cells are thought to down-modulate the response [2, 30, 31]. The deleterious expansion of Ni-specific, effector CD8+ T cells might be thus prevented by regulatory mechanisms, including those induced by hapten-specific suppressor T cells [32]. In humans IL-10 has been identified as a potent anti-inflammatory cytokine produced by both Th1 and Th2 CD4+ T cells [33], which inhibits T cell proliferation of both subsets, and regulates the development of Th1 cells by blocking IL-12 driven Th1 responses [34]. IL-10 is thought to exert its effects by down-regulating the expression of co-stimulatory molecules required for appropriate antigen presentation [35]. Ferguson TA et al and Schwarz A et al [36, 37] demonstrated a key regulatory role for IL-10 in both the induction and the effector phases of murine contact hypersensitivity. Our results provide evidence supporting the hypothesis that a regulatory mechanism mediated by IL-10 contributes to the control of hypersensitivity in response to Ni, Pd and Rh therefore contrasting the development of ACD. Even if the protective role of IL-10 is well established, studies addressing the cytokine profile in subjects with or without ACD have been partly contradictory [26, 31, 38]. It should be considered that different protocols used for the in vitro stimulation or the different sensitivities of the immunoassays for IL-10 measurements could be responsible for the different results obtained.
The finding that not only Ni but also other metals could elicit a response in PBMC from sensitized subjects [15], was not surprising and prompted us to explore the profile of cytokine production in patients which scored negative at patch testing toward Pd and Rh, which are present in several synthetic materials including new metallic alloys [39, 40]. Remarkably, we found very clear-cut, divergent, IL-10 "protective" or IFN-γ "reactive" profiles in patients showing a negative or positive skin reactivity to metal salts (group 1, 2, 4), respectively. Moreover, all the subjects with positive patch test to both Ni and Pd (group 3) showed an in vitro response similar to monosensitized patients to Ni, characterized by the release of IFN-γ after Ni stimulation and production of IL-10 in response to Pd or Rh. Interestingly, comparing the cytokine response to Pd, subjects with the same patch test reactivity (n = 3: ++ in the group 2; n = 6: ++ in the group 3) displayed a reversed profile (Table 2). On the basis of these data, it is conceivable that a positive (IFN-γ) response in vitro may predict the development of adverse reaction to prosthesis alloys or to chemotherapeutic agents containing toxic metals. In fact, previous studies on multiple sensitivities to transition metals reported that the majority of Ni/Pd reactions occurred without clinical evidence of Pd sensitization [10, 19, 41].
We analyzed the IFN-γ response in patients with an unambiguous Ni patch test reaction (group 1 and 3 patients with +++ reactions) to provide positive controls for the in vitro assay, in order to define optimal conditions for antigen-specific cytokine production and to offer a highly sensitive and specific test suitable for clinical determinations. On the basis of this setting, stimulation values greater than 3 to 7 were commonly obtained with metal salts or with lectin (PHA) polyclonal stimulation, respectively, suggesting that cryopreservation did not affect the cytokine response of lymphocytes, as sustained by the study from Kreher et al [42].
The data gathered by the ELISpot analysis did not show any cross-reactivity of T-cells neither the co-existence of T cell clones with differential responsiveness. Since any Ni contamination was demonstrated in the Pd salt used for patch testing (data not shown), it is presumable that an immunologic cross-reactivity based on molecular mimicry among different hapten-carrier complexes might take place in vivo, at the skin level. Due to their small size, metal ions are incomplete antigens which have to bind to carrier molecules, which, in turn, are capable to capture metal ions, and shuttling this complex to the epidermal basement membrane [4, 7]. On the other hand, the concomitant reactivity to Ni and Pd, observed in a number of patients by skin testing could concern also the possibility of a delayed or impaired response of skin regulatory cells. A dysfunction in T-regulatory cell can lead to an immune activation often associated with the clinical expression of skin diseases such as atopic dermatitis and psoriasis [43]. Experimental data suggested also the possibility that, in analogy to superantigens, Ni or Pd may also directly link TCR and MHC in a peptide independent manner and can effectively amplify the hapten-specific immune response [44, 45]. It is also known that a strong binding of different superantigens to the TCR could render T cells insensitive to suppression by CD4+CD25+ Treg cells [46] and IL-10 [47].
Knowledge about the immunologic mechanism responsible for ACD is still fragmentary. It is accepted that the sensitising chemical penetrates into the epidermis, and, depending on its properties, it "perturbates" the environment. However, neither the molecular nature of Ni-induced antigenic determinants nor the very early molecular events of metal transport through the human epidermis to Langerhans cells have yet been satisfactorily resolved indicating that still many aspects of metal allergy remain unclear.
Conclusion
Taken together, these results demonstrate that different cytokine profiles elicited in vitro can reflect different immune responses in vivo in ACD. The development of sensitive and specific in vitro assays based on cytokine response could be extremely useful in complementing patch testing for the clinical diagnosis and characterization of metal sensitization.
Methods
Patients
Fourty patients (n = 36 female (f)/4 male (m); mean age 33.3 yrs, range between 28–41) attending the Department of Allergy of this Institute were enrolled in the study. Patient assessment was primarily based on a detailed medical history, including history of metal exposure. Wearing of metal dental braces was also recorded. None of the patients had recently used immunosuppressive medication, underwent UV radiation or suffered from acute inflammatory skin diseases. The study received the approval from the Internal Ethic Committee of Istituti Fisioterapici Ospitalieri (San Gallicano, IFO, Rome), and an oral informed consent was obtained from all subjects prior to the in vitro study.
Patch testing
Patch testing was performed with all patients enrolled in the study. The skin patch tests were with the European standard series of contact allergens (Hermal Trolab, Reinbeck, Germany), including 5% Nickel Sulphate hexahydrate (NiSO4 × 6H2O) applied in petrolatum, and supplemented with 1% Palladium Chloride (PdCl2) applied in petrolatum and 1% Rhodium Acetate Rh(CH3COO)2 in aqueous solution. These salts were from Merck (AG, Darmstadt, F.R.G). The patch tests were performed by applying Finn Chambers with haptens on unaffected skin of the upper backs of the subjects for 48 h. Patch test responses were examined on day 2 and defined as strong (+++: oedema, erythema, papules and vesicles), moderate (++: oedema, erythema and papules), weak (+: oedema and erythema) or no reaction (-) according to the International Contact Dermatitis Research Group guidelines [48].
ELISpot assay
Peripheral blood mononuclear cells
PBMC were isolated from 5 to 10 ml of heparinized blood, collected 48 hrs after skin testing, by standard Ficoll density-gradient centrifugation (Lympholyte-H solution Cederlane, Ontario, Canada) and washed twice with PBS. Cell aliquots were frozen in 90% heat inactivated Fetal Bovine Serum (FBS, Euroclone) and 10% DMSO (Dimethylsulphoxide, Sigma) and kept in liquid nitrogen until tested for ELISpot assay.
Metal salts used for in vitro cytokine assay
The metal salts used for in vitro assays were: nickel sulphate hexahydrate NiSO4 × 6H2O, palladium chloride (PdCl2) and rhodium acetate Rh(CH3COO)2 (Merck AG, Darmstadt, F.R.G). Before use, metals were resuspended in sterile saline solution at 2 mg/ml (Bioindustria, Novi Ligure, Italy). These stock solutions were found negative for LPS contamination (Limulus assay, BioWhittaker, Cambrex Company, USA).
Definition of optimal metal salt concentrations for antigen-specific cytokine production
Preliminary experiments were performed in order to determine the optimal concentration of each metal salt for IL-10 or IFN-γ producing cells induction, measured by ELISpot assay. Briefly, 3.5 × 105 PBMC/well from 5 non-allergic donors were stimulated with 10 μg/ml of phytohaemagglutinin (PHA, Sigma, Saint Louis, Missouri, USA) in the presence of different metal salts at concentrations ranging from 0.1 μg/ml to 100 μg/ml. Metal salt concentrations > 50 μg/ml, > 25 μg/ml and > 30 of Ni, Pd and Rh respectively inhibited the PHA-induced cytokine producing cells by 25%, and were considered toxic in agreement with other authors (15). Subsequent preliminary experiments showed that 20 μg/ml NiSO4 or 2.5 μg/ml PdCl2 yielded the higher frequency of IFN-γ producing cells in five subjects with clinical diagnosis of ACD caused by Ni (n = 3) or Pd (n = 2) respectively. While, PBMC from five healthy controls cultured at the same experimental conditions provided the higher frequency of IL-10 producing cells. Also the Rh(CH3COO)2 concentration of 5 μg/ml was chosen on the basis of the higher frequency of IL-10 producing cells in patients as well as controls.
Pre-incubation with metals
For the detection of IFN-γ, PBMC (3.5 × 105/well) were pre-incubated with or without metals in round bottom polypropylene tubes (Becton Dickinson Labware) in complete medium (RPMI 1640, 10% FBS, 1% Penicillin-Streptomycin-L-Glutamine; GIBCO-BRL, UK) for 20 hrs at 37°C and 5% CO2 in humidified air. Then cells were transferred to the ELISpot plates and incubated for a further 48 hrs.
ELISpot assays were performed as previously described [49] with minor modifications. Briefly, Polyvinylidene difluoride-backed 96-well microtiter plates (MAIPS4510; Millipore Sunnyvale, CA, USA) were coated overnight at 4°C with 100 μl/well of specific capture antibody anti-IFN-γ or anti-IL-10 (10 μg/ml, 100 μl/well, Endogen Woburn, MA) dissolved in phosphate buffered saline (PBS).
Ab-coated plates were then washed three times and incubated 1 h at 37°C with complete medium to prevent non-specific protein binding. PBMC were plated at 3.5 × 105/well, to a final volume of 200 μl/well of complete medium. Three different concentrations of NiSO4 (5–10–20 μg/ml), PdCl2 and Rh(CH3COO)2 (2.5–5–10 μg/ml) were added in triplicate wells and plates were kept in a humidified atmosphere with 5% CO2 at 37°C. Control triplicate wells contained PBMC with medium alone or with phytohaemagglutinin (PHA 1 μg/ml, Sigma, Saint Louis, Missouri, USA). After 48 hrs for IFN-γ and 72 hrs for IL-10 detection, cells were lysed with ice-cold distilled water, and cells were removed by 4 rinses with PBS/0.05% Tween® 20 (Sigma, St Louis, MO, USA). After a 90 min incubation with biotin labeled anti-IFN-γ or anti-IL-10 detection antibodies (1 μg/ml, 100 μl/well, Endogen Woburn, MA), HRP-Streptavidin-alkaline phosphatase (Euroclone, PBS 1% BSA) was added to the wells at a dilution of 1:1000 for 30 min at 37°C in the dark. Subsequent to specific substrate addition, the red spots were developed using AEC-chromogen solution (Sigma, St Louis, MO, USA) and analysed by the Automated ImmunoSpot Image Analyzer Software (AELVIS Tecnologies, TEMA ricerche, Italy). The results were expressed as the ratio (Index) between the number of spot forming cells detected upon stimulation and those present in the absence of stimuli. A positive response is defined by an Index > 3.
Statistical analysis
The Mann-Whitney (MW) rank sum test was used to compare the cytokine responses between two different groups of patients. To evaluate the correlation between IFN-γ and IL-10 producing cells, the Spearman rank coefficient r was considered. A P value < 0.05 was considered to be statistically significant. Statistical analysis was performed with GraphPad Software, version 4.00 (San Diego California USA).
Declarations
Authors’ Affiliations
(1)
Department of Allergology and Laboratory of Skin Physiopathology, Laboratory of Clinical Pathology, Institute San Gallicano, IRCCS, IFO
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