Fig. 2

Capparis Spinosa crud extract and aqueous fraction did not affect proliferation of human PBMCs. a CFSE-stained PBMCs stimulated with PHA 5 μg/ml. b CFSE-stained PBMCs in the absence of stimulation. c, d, and e an example of curves generated by CFSE-stained PBMC, stimulated with PHA 5 μg/ml and harvested with 10, 100 and 500 μg/ml respectively of aqueous fraction, f, g and h an example of curves generated by CFSE-stained PBMC harvested with of 10, 100 and 500 μg/ml of aqueous fraction, for 4 days. i, j and k An example of curves generated by proliferation of CFSE-stained PBMC harvested with 10, 100 and 500 μg/ml of crud extract for 4 days of culture. l and m An example of curves generated by CFSE-stained and stimulated PBMC in the presence of 10 and 100 μg/ml respectively of the crud extract, for 4 days. n and o Cumulative data from five independent experiments analyzed statistically using one-way ANOVA test. (***) indicate a P value of less 0.001 respectively. Each peak represents a cycle cell division. The curves generated by the CFSE profile were analyzed using the proliferation platform of the FlowJo software. Data shown represent results of five independent experiments