Fig. 3

No significant the changes of transcription of NKG2DLs by combined treatment of TGF-β and Galunisertib in lung cancer cells. The transcription levels of NKG2DLs were analyzed in (A) NCI-H23, (B) SW-900 and (C) A549 lung cancer cells by multiplex RT-PCR. The flung cancer cells were treated with TGF-β (10 and 20 ng/ml) and Galunisertib (5 and 10 μM) for 18 h. The PCR products were separated and quantified by MultiNA. All experiments were done in triplicate. Changes of transcriptions were normalized by ACTB and presented as the mean fold changes compared to media treated groups. The experiments were performed three times