Table 2 PCR primers used in this study
From: A survey of the adaptive immune genes of the polka-dot batfish Ogcocephalus cubifrons
Target | Primer 1 | Primer 2 | Use |
|---|---|---|---|
cd4.1, exons 1–3 | TCTTTGGTGTGCTTTGTGCC | TGTGCGTCCTCTTACCACTG | amplification and cloning of cd4.1 variant region |
mhc1 | GAGGAGGGCGGATAAACGAG | TCAGGGTGGTGTGTTTGTCG | amplification and cloning of mhc1 variants |
mhc2b | CATGGCTTCATCCTGTCTCACC | GATCAGGTCCAGGACTGCTC | amplification and cloning of mhc2 variants |
rag1 | GTTCGCAGACGAGAGGGAG | GAGGAACGTGTTGACCCGAA | verification of genomic assembly |
rag1 – rag2 interval | TGATGGATGGTTCCTCGCTC | TTTAGGGAGGGTCCGTGTGA | verification of genomic assembly |