- Research article
- Open Access
YopH inhibits early pro-inflammatory cytokine responses during plague pneumonia
© Cantwell et al; licensee BioMed Central Ltd. 2010
- Received: 18 November 2009
- Accepted: 16 June 2010
- Published: 16 June 2010
Yersinia pestis is the causative agent of pneumonic plague; recently, we and others reported that during the first 24-36 hours after pulmonary infection with Y. pestis pro-inflammatory cytokine expression is undetectable in lung tissues.
Here, we report that, intranasal infection of mice with CO92 delta yopH mutant results in an early pro-inflammatory response in the lungs characterized by an increase in the pro-inflammatory cytokines Tumor Necrosis Factor-alpha and Interleukin one-beta 24 hours post-infection. CO92 delta yopH colonizes the lung but does not disseminate to the liver or spleen and is cleared from the host within 72 hours post-infection. This is different from what is observed in a wild-type CO92 infection, where pro-inflammatory cytokine expression and immune cell infiltration into the lungs is not detectable until 36-48 h post-infection. CO92 rapidly disseminates to the liver and spleen resulting in high bacterial burdens in these tissues ultimately cumulating in death 72-94 h post-infection. Mice deficient in TNF-alpha are more susceptible to CO92 delta yopH infection with 40% of the mice succumbing to infection.
Altogether, our results suggest that YopH can inhibit an early pro-inflammatory response in the lungs of mice and that this is an important step in the pathogenesis of infection.
- Bronchial Epithelium
- Yersinia Pestis
- Bacterial Burden
- Intranasal Infection
Yersinia pestis is a Gram-negative bacterium with a zoonotic life cycle that occasionally results in human infections leading to plague . Plague manifests in two major forms, the most common being bubonic plague. Bubonic plague is transmitted to humans through the bite of an infected flea, resulting in intradermal inoculation of the bacterium, which progresses to form the characteristic lymphadenitis (bubos) of plague . The less common but contagious form of the disease is pneumonic plague, which is a severe pneumonia resulting either from inhalation of infectious respiratory droplets, or secondary to bubonic or septicemic plague. Pneumonic plague is highly aggressive and if untreated, is able to kill the host within 2-4 days post-exposure, with mortality rates that approach 100% [1–3].
We and others have begun to analyze the immune response to primary pneumonic plague using wild-type strains of Y. pestis in an effort to understand the interactions of Y. pestis with its host [4, 5]early during the development of disease. The rapid progression of the disease and associated mortality suggests that subversion of innate immunity plays a key role in disease development . Our previous studies indicate a delay in the host inflammatory response to infection, resulting in an opportunity for the bacterium to replicate to high numbers and potentially overwhelm the host immune system, rapidly resulting in death . Modulation of host-immune responses is a common pathogenic mechanism among the virulent species of Yersinia and much work has been done to understand the molecular mechanisms underlying the virulence factors involved in this process [6–8].
The 70 Kb virulence plasmid (pCD-1), which is essential for virulence, contains all of the machinery and effector proteins for a type-three secretion system (TTSS) [6, 8]. The secreted effector proteins are also known as the Yersinia outer proteins (Yops) and are transported from the bacterial cytosol through the TTSS into the cytoplasm of a host cell to facilitate infection. All six of the effector Yops have been studied, and at least one function has been assigned to each based on in vitro studies. Several are involved in manipulating the host cytoskeleton (YopE, YopH, YopT, YopO); these yops interfere with the Rho family of GTPases and other host proteins involved in the regulation of the cytoskeleton, and are therefore important in the modulation of phagocytosis [9, 10]. Others are involved in the tempering of the host immune response, in particular, YopH and YopJ, and to a lesser extent, YopE and YopM have been shown to impact inflammatory cytokine expression in vitro and in vivo respectively [8, 11].
YopH is a protein tyrosine phosphatase, which is known to interact with p130cas and FAK to impair invasion of epithelial cells by Yersinia pseudotuberculosis [12, 13]. YopH blocks cytoskeletal rearrangement upon injection into the host cell, contributing to the decreased ability of macrophages and other immune cells to phagocytose the bacteria . Distinct from its ability to interact with the cytoskeleton, YopH is also known to impact host cell signaling by inactivation of the PI3K pathway through unknown mechanisms . The PI3K pathway plays an important role in the macrophage response to infection including generation of the oxidative burst and nitric oxide production, phagosome formation, and has a role in a negative regulation of IL-12 production, to help keep the inflammatory response in check .
Numerous studies have determined that mutations in YopH severely impact the virulence of the yersiniae [17–19]. Recently we reported that CO92ΔyopH is severely attenuated in both an intranasal and subcutaneous models of Y. pesti s infection, which makes CO92ΔyopH a very good live-attenuated vaccine strain . The ability of CO92ΔyopH to provide significant protection against virulent challenge suggests that a robust protective immune response is generated during primary infection with this strain. However, the role of YopH in the pathogenesis of plague pneumonia or its impact on the immune response in the lung has not been examined. In this study we test the impact of the CO92ΔyopH mutant on both the virulence and inflammatory response using a mouse model of primary pneumonic plague.
Y. pestis CO92ΔyopH is severely attenuated in an intranasal model of primary pneumonic plague
Attenuation of CO92ΔyopH is due to the loss of YopH and YopH enzymatic activity
Y. pestis CO92ΔyopH is able to persist in the lungs but does not spread systemically
Intranasal infection with CO92ΔyopH induces TNF-α and IL-1β early during infection
Mice lacking proinflammatory cytokines are more sensitive to CO92ΔyopH infection
Histopathology of CO92ΔyopH infection
Recently, we and others described the development of primary pneumonic plague in the mouse following infection with CO92 [4, 5]. The most striking feature of primary plague pneumonia is a 36-hour delay in the inflammatory response to infection, which is characterized by rapid increases in bacterial burden, a lack of inflammatory cytokine and chemokine expression, and scant evidence of inflammation in histopathological examination of lung tissues. CO92ΔyopH infection presents a significantly different picture: 1) there is little bacterial replication in the lungs (Fig. 3), 2) CO92ΔyopH is severely attenuated in both bubonic and pneumonic plague models (Fig. 1A &1B and ), and 3) TNF-α and IL-1β are readily detectable in the BALF of mice infected with CO92ΔyopH at 24 hours post-infection (Fig. 4A &4B) suggesting that CO92ΔyopH induces a detectable inflammatory response in the lung. Altogether, these data would suggest that the histopathology of CO92ΔyopH might be different than that observed during a CO92 infection.
At 48 h post infection the histopathology of the CO92ΔyopH infection remains very different than that observed during a CO92 infection. The wild-type infection is characterized by large areas of pulmonary consolidation (not shown), a robust inflammatory response composed of mostly of PMNs (Fig. 7D, black arrow), fibrin, exudates in conducting airways (Fig. 7D), and abundant free bacteria (Fig. 7E, black arrow). The inflammatory response to the CO92 infection at 48 h post-infection also includes multiple areas of overt extracellular bacterial growth interspersed with a large number of dead host-cells and cellular debris. These findings were observed in 100% of the mice infected with CO92 for 48 h. CO92ΔyopH exhibits several prominent findings at the 48 h time point (Fig. 7F-H). Large pronounced inflammatory lesions composed mostly of PMNs were present in 50% of the mice investigated at this time point (Fig. 7G and 7H). These lesions are different than those observed in the wild-type infection in that bacterial growth is not apparent (Fig. 7G), there is less cell death and cellular debris (compare Fig. 7D and 7G), and there is a pronounced infiltration of the bronchial mucosa with inflammatory cells (Fig. 7H). The other type of lesion common in the CO92ΔyopH infected mice is exemplified in Figure 7F and consists of severe vacuolization of the bronchial epithelium. In contrast to what is observed at 24 h (Fig. 7C), necrosis of the bronchial sub-mucosa is apparent in areas adjacent to vacuolated epithelium in 50% of the animals examined (Fig. 7F, black arrow). Unlike the clear airways observed 24 h after CO92ΔyopH infection, at 48 h post-infection, there are exudates composed of PMNs, macrophages, and sloughed epithelial cells (Fig. 6F). However, bacteria were not evident in the airways of any of the animals infected with CO92ΔyopH. Altogether these data suggest that CO92ΔyopH induces a robust inflammatory response prior to what is observed with CO92 infection. Further, CO92ΔyopH causes significant vacuolization of the bronchial epithelium that is only occasionally observed during a CO92 infection.
We investigated the effect of YopH deletion on the virulence of Y. pestis using a murine model of primary pneumonic plague. YopH, a type-three secretion system effector protein, is a protein tyrosine phosphatase that is involved in inhibiting phagocytosis [14, 17], as well as blocking the PI3-kinase pathway . Investigators have studied the effect of YopH deletion using multiple species of Yersinia, including Y. pestis yopH pgm double mutants utilizing several routes of infection [11, 15, 17–21]. To date most yopH mutants tested have exhibited decreased virulence and interestingly, lung infections of Balb/c mice with a yopH mutant of the closely related Yersinia pseudotuberculosis resulted in a strong attenuation phenotype . However, this has never been tested in a murine model of primary pneumonic plague using WT Y. pestis strains.
The attenuation of CO92ΔyopH is due to the loss of the protein because virulence can be restored when the CO92ΔyopH mutant is complemented with a wild type copy of the YopH gene. Further, it is the actual phosphatase activity of YopH that is required for virulence since a CO92ΔyopH mutant complemented with a yopH gene containing an enzymatic inactivating mutation (C403A) is completely attenuated. This is an intriguing finding suggesting that small molecule inhibitors of the YopH phosphatase activity might be useful therapeutics for the treatment of plague.
In addition to evaluating the virulence of CO92ΔyopH, we examined the host response to pulmonary infection by testing for differences in pro-inflammatory cytokines expression and lung histopathology. We hypothesized that there could be a host component that contributed to the attenuation of the CO92ΔyopH mutant. It has been observed by us and others that infection with Y. pestis CO92 leads to a delayed inflammatory response, which results in very high bacterial loads in the lungs, liver and spleen, ultimately leading to the death of the host [4, 5, 26]. We speculated that alleviation of the anti-inflammatory mechanisms of Y. pestis would provide protection for the host.
In the absence of YopH, the host responds to the presence of Y. pestis with an early pro-inflammatory response, namely, an increase in TNF-α and IL-1β in the BALF at 24 h post-infection, which diminishes in magnitude by 48 h post-infection. This is different than what is observed with CO92 infection, where an inflammatory response is not observed until 48 h post-infection . In addition, the kinetics of bacterial growth following an intranasal infection with CO92ΔyopH is different than the kinetics of CO92 intranasal infection. First, CO92ΔyopH is able to persist in the lungs of infected mice, although the bacterial burdens observed are significantly lower than those of CO92 at both at 24 h and 48 h post-infection. It appears that the CO92ΔyopH is unable to propagate in the lungs of the host, as demonstrated by the fact that the amount of bacteria detected in the lungs remains approximately the same at 24 h and 48 h post-infection. These data are similar to what is observed with a pulmonary infection with the Y. pseudotuberculosis yopH mutant . Additionally, CO92ΔyopH is unable to disseminate from the site of infection to either the liver or spleen at 24 h, 48 h, 72 h, or 96 h post-infection. In contrast, infection with wild-type CO92 results in dissemination and high bacterial burdens in the liver and spleen at 48 h post-infection, and the majority of the animals are dead by 72 h post-infection . Viable CO92ΔyopH is cleared from the host by 72 h post-infection being undetectable in the lung, liver, or spleen of mice 72 h and 96 h post-infection (data not shown). In light of these data, we hypothesize that the early pro-inflammatory response observed during infection with CO92ΔyopH is key in allowing the host to adequately control the bacteria and prevent a fulminate infection.
Additional evidence for this conclusion lies in the differences observed in the histopathology of lungs from mice infected with either wild type CO92 or CO92ΔyopH at both 24 h and 48 h post-infection. The lung histopathology of these two infections is strikingly different and provides some insight into the dissimilar courses that these infections follow. At 24 hours post-infection with wild-type Y. pestis, there are only subtle changes in the lung tissue with very little tissue damage. Infection with CO92ΔyopH at this same time post-infection leads to widespread vacuolization of bronchial epithelium and infiltration of inflammatory cells. It is unclear what leads to the vacuolization, but it is possible that the cyto-toxic effects of YopE and YopO are amplified in the yopH mutant [6, 8, 27]. After 48 hours of infection with CO92, evidence of severe lung pathology is evident including pulmonary consolidation and a heavy infiltration of inflammatory cells comprised predominantly of PMNs. In addition, free bacteria are readily observed throughout the lung tissue. In CO92ΔyopH infection, at 48 h post-infection, about 50% of mice exhibit large lesions with PMNs, but strikingly, no bacteria are observed. This provides further evidence that an early inflammatory response to infection with CO92ΔyopH might be protective, and contribute to the clearance of Y. pestis prior to outgrowth and dissemination of the bacteria.
Other investigators recently observed that when innate immunity and specifically inflammatory immune responses are intact, infection with Y. pestis does not cause severe disease. For example, Y. pestis modifies its LOS when growing at 37°C, using a tetra-acylated lipid A, which only weakly induces a pro-inflammatory response in the host. At 21-27°C, Y. pseudotuberculosis produces a hexa-acylated lipid A, the form of lipid A which is able to elicit potent inflammatory responses from the host . The tetra-acylated lipid A has poor Toll-like receptor 4 (TLR-4) stimulating activity and therefore does not elicit the same robust pro-inflammatory response induced by hexa-acylated lipid A . When Montminy et al genetically modified Y. pestis to produce the more potent hexa-acylated lipid A at 37°C, they found that mice did not develop bubonic plague when infected subcutaneously even at doses approaching 106 mean lethal doses . These data indicate that the TLR-4 response is critical in overcoming infection with Y. pestis and that by avoiding this response through LPS modification, Y. pestis is able to cause disease in the host . A second study demonstrating the importance of an active inflammatory response early in Y. pestis infection showed that when mice were latently infected with gamma-herpes virus there is a significant increase in activated circulating macrophages and the severity of subsequent infection with fully virulent Y. pestis was decreased compared to mice which were mock infected with virus . Consistent with the data presented in this study, TLR-4 signaling and activated macrophages are capable of producing and responding to IL-1β and TNF-α to fight against bacterial infection.
YopH is a complex virulence factor that impacts many aspects of the pathogenesis of Yersinia sp. Given that YopH is a potent protein tyrosine phosphatase, it is well suited to disrupting host signal transduction pathways. It is interesting to note that the majority of evidence obtained studying the enteropathogenic Yersinia suggests that YopH acts to diminish integrin signaling following invasin binding [7, 12, 17, 31, 32]. The result of this is that Y. enterocolitica and Y. pseudotuberculosis are predominantly extracellular pathogens. In contrast, Y. pestis CO92 does not express invasin , and is a facultative intracellular pathogen but YopH is a required virulence factor suggesting it has additional roles in Y. pestis pathogenesis.
We hypothesize that the anti-host activities of YopH are beneficial to the survival of Y. pestis within the mammalian host and that YopH contributes to the virulence of the organism by preventing an early pro-inflammatory response at the site of infection; thereby allowing the bacteria to rapidly replicate in the organs of infected mice, overwhelming and killing the host. YopH directly impacts these key aspects of the pathogenesis of plague pneumonia. However, given the number of potential host-pathways that could be targeted by YopH, it is likely that this virulence factor impacts multiple aspects of Y. pestis pathogenesis.
Pathogenic Yersinia have evolved numerous mechanisms to survive in their hosts and many of the proteins that support survival in the host are virulence factors. Many of the most potent virulence factors are encoded on the 70 Kb virulence plasmid pCD-1 and several of them have been shown to be critical for causing disease. Amongst the essential plasmid-encoded virulence factors is YopH a protein tyrosine phosphatase that has been shown to be critical for the inhibition of invasion by the enteropathogenic yersiniae. Interestingly, Y. pestis is a facultative intracellular pathogen that does not express the Inv protein suggesting that Y. pestis YopH plays additional roles in the pathogenesis of this pathogen.
A mutation in yopH leads to severe attenuation of virulence in mouse models of primary plague pneumonia. In this study, our data suggests that YopH inhibits the production of IL-1β and TNF-α during the first 24 hours post-infection. The inhibition of IL-1β and TNF-α is a critical step in the pathogenesis of infection because mice deficient in these molecules are partially sensitive to infection with the yopH mutant. Further, the YopH phosphatase activity is essential for virulence because mice infected with the CO92ΔyopH strain complemented with a wild type copy of the yopH gene succumb to infection with kinetics similar to CO92 infection but complementation with the yopH-C403A gene restores complete attenuation. Altogether our data suggests that YopH plays an important role in the subversion of innate immunity during plague pneumonia.
Yersinia pestis CO92, a biovar Orientalis strain recently isolated from a case of pneumonic plague, was obtained from the Select Agent Distribution Activity (SADA), Centers for Disease Control and Prevention (CDC), Fort Collins, CO. The strain was confirmed to contain the pigmentation (pgm) locus phenotypically by producing red colonies on Congo red plates and by polymerase chain reaction (PCR). The presence of the low calcium response virulence plasmid (Lcr) was confirmed by PCR of the lcrV, yopH, and yopJ genes. Virulence in mice was confirmed as described below. The yopH deletion mutant was created in Y. pestis strain CO92 using the suicide vector pSR47s , a derivative of pSR47 , as described . The yopH mutation was complemented by cloning the CO92 yopH gene and 247bp of 5' UTR into the pCR2.1 plasmid creating pAMC-1. Complemented strains were shown to produce equivalent amounts of YopH after in vitro induction of TTS as described . The YopH-C403A active site mutant was produced on the pAMC-1 plasmid background using the quick-change method as we have previously described creating pAMC-2 . YopH was detected in the TCA precipitated culture supernatant and in whole cell extracts by immunoblot using a rabbit polyclonal anti-YopH antibody kindly provided by James Bliska (SUNY Stony Brook).
Six to eight-week old female out-bred CD1 mice (Charles River Laboratories, Willmington, MA) were used for most studies and processed as we have previously described [4, 18]. TNF-α deficient mice (B6;129S-Tnftm1Gkl/J stock #003008) and controls (B6129SF2/J stock #101045) were obtained from Jackson Laboratory (Bar Harbor, ME) and used at 6 weeks of age. Fully virulent Yersinia pestis strain CO92 or CO92ΔyopH was grown for 20 hours at 28°C in Heart Infusion broth (US Biological, Swampscott, MA) supplemented with 0.2% xylose. Cultures were then harvested and washed once with sterile PBS, then diluted in endotoxin-free PBS to approximately 106 bacteria/mL. Bacterial concentrations were verified by enumerating colony forming units (CFU) on Congo red plates. Mice were anesthetized by intraperitoneal injection of 0.5 mL Avertin (20 mg/mL in PBS, 2-2-2 Tribromoethanol, Sigma, St. Louis, MO) and then intranasally infected with 20 uL of inoculums (10 uL/nare). Intradermal infections were performed by injection of the appropriate dilution of culture in 50 μl volume into the left ear using an insulin syringe as described . Actual CFU's for individual experiments were determined by plating serial dilutions of the inoculums and are reported in the subsequent text and graphics. Mice were euthanized by overdose of isoflurane (Iso-Thesia, Vetus Animal Health, Burns Veterinary Supply, Inc., Westbury, NY) and then cervical dislocation at 24, 48, 72, or 96 hours after infection as indicated. For mice used to determine the virulence of CO92ΔyopH, mice were inoculated with up to 2×107 CFU/mouse, and survival was monitored daily over a period of 10-14 days depending on experimental design. All experiments using Y. pestis were performed at Biosafety Level 3, in accordance with approved Institutional Biosafety Committee and Institutional Animal Care and Use Committee protocols.
The bacterial burden in target organs of mice infected with either Y. pestis CO92 or CO92ΔyopH was determined as previously described . Briefly, lungs, liver, and spleen were harvested at the indicated times and weighed prior to homogenization. Tissue homogenates were then diluted in PBS and dilutions were plated on Congo red plates. Bacterial growth was evaluated after 72 hours incubation at 28°C. Results are presented as CFU/gram of tissue and representative data from two independent experiments with at least six mice per data point is shown.
In vivo Cytokine Depletion
IL-1β and TNF-α were depleted by antibody-mediated ablation as we described previously . Briefly, CD1 mice were injected IP with 0.25 mg of rat anti-mTNF-α (clone MP6-XT22, e-Biosciences), hamster anti-mIL-1β (clone B122, e-Biosciences) or a mixture of 0.25 mg of both antibodies one day prior to infection with CO92 or CO92ΔyopH and then every third day until the end of the experiment. Control animals received an equivalent concentration of isotype-matched irrelevant rat or hamster IgG respectively. Mice were then monitored daily for survival. Data represents experiments with 10 mice per group.
Mice were euthanized and a 1 cm longitudinal incision was made to expose the trachea. Bronchoalveolar lavage (BAL) was performed by catheterizing the trachea using 18 gauge catheters (Beckton, Dickinson Infusion Therapy Systems, Inc., Sandy, UT). Each mouse was lavaged with three 1 mL aliquots of PBS with protease inhibitors (pepstatin, PMSF, aprotinin, leupeptin, Sigma, St. Louis, MO). BAL fluids (BALF) were placed immediately on ice, filtered with a 0.2 μm syringe filter (SFCA, Fisher Scientific, Pittsburg, PA), and stored at -80°C for future analysis. The results are representative of two independent experiments with four to six animals per bacterial strain and time point.
BALF samples or tissue culture supernatants were diluted as appropriate and used in ELISA assays for mouse TNFα and IL-1β (BD Biosciences Pharmingen, San Diego, CA) following the manufacturers instructions. Eight to twelve samples per data point were used in the assays; each sample was assayed in duplicate.
Following IN infection, the lungs were harvested at the indicated times and their gross appearance was evaluated at necropsy. At least ten animals per data point were examined. Tissues were fixed in 10 ml of 10% neutral buffered formalin (NBF). Formalin-fixed tissues were then embedded in paraffin and 3-4 μm sections were cut and placed on slides. The tissue sections were then stained with hematoxylin and eosin (H&E). Tissues were examined from at least eight mice per time point and evaluated in blind fashion for inflammatory cell infiltration, bacterial colonization, and presence of inflammatory exudates in airways, edema, necrosis, hemorrhage, and fibrin. Images were captured digitally on a Zeiss Axioscope 2 microscope equipped with a digital camera. Images were processed using the Axiovision V.4 suite of software (Carl Zeiss, Inc., Thornwood, NY).
All results were expressed as the mean ± SEM. Statistical differences were determined using either a two-tailed Student's t test or Mann-Whitney non-parametric test as indicated using GraphPad In-Stat3 (GraphPad Software). Survival data was analyzed by log-rank analysis where possible. A value for p < 0.05 was considered significant.
The National Institutes of Health through grants AI 060789 and AI 067716 awarded to PHD supported this work. The grantor had no role in manuscript preparation, study design, or data analysis/interpretation.
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