- Research article
- Open Access
Characterisation of RT1-E2, a multigenic family of highly conserved rat non-classical MHC class I molecules initially identified in cells from immunoprivileged sites
© Lau et al; licensee BioMed Central Ltd. 2003
- Received: 10 February 2003
- Accepted: 01 July 2003
- Published: 01 July 2003
The Erratum to this article has been published in BMC Immunology 2004 5:4
So-called "immunoprivileged sites" are tissues or organs where slow allograft rejection correlates with low levels of expression of MHC class I molecules. Whilst classical class I molecules are recognised by cytotoxic T lymphocytes (CTL), some MHC class I molecules are called "non-classical" because they exhibit low polymorphism and are not widely expressed. These last years, several studies have shown that these can play different, more specialised roles than their classical counterparts. In the course of efforts to characterise MHC class I expression in rat cells obtained from immunoprivileged sites such as the central nervous system or the placenta, a new family of non-classical MHC class I molecules, which we have named RT1-E2, has been uncovered.
Members of the RT1-E2 family are all highly homologous to one another, and the number of RT1-E2 loci varies from one to four per MHC haplotype among the six rat strains studied so far, with some loci predicted to give rise to soluble molecules. The RT1 n MHC haplotype (found in BN rats) carries a single RT1-E2 locus, which lies in the RT1-C/E region of the MHC and displays the typical exon-intron organisation and promoter features seen in other rat MHC class I genes. We present evidence that: i) RT1-E2 molecules can be detected at the surface of transfected mouse L cells and simian COS-7 cells, albeit at low levels; ii) their transport to the cell surface is dependent on a functional TAP transporter. In L cells, their transport is also hindered by protease inhibitors, brefeldin A and monensin.
These findings suggest that RT1-E2 molecules probably associate with ligands of peptidic nature. The high homology between the RT1-E2 molecules isolated from divergent rat MHC haplotypes is particularly striking at the level of their extra-cellular portions. Compared to other class I molecules, this suggests that RT1-E2 molecules may associate with well defined sets of ligands. Several characteristics point to a certain similarity to the mouse H2-Qa2 and human HLA-G molecules.
- Immunoprivileged Site
- Central Nervous System Architecture
- Rodent Class
Mature MHC class I molecules are heterotrimers composed of a heavy chain polypeptide, a light chain (β2-microglobulin) and a small molecular weight 'ligand' that is usually, but not always, peptidic in nature. In all vertebrate species studied to date, MHC class I heavy chains are encoded by several genetic loci, some of them highly polymorphic. The principal site of variation is the ligand-binding groove, formed at the apical face of the molecule by the α1-α2 co-domain of the heavy chain. Most, but not all of the MHC-encoded class I molecules characterised to date are endowed with a role in presentation of antigenic ligands to cells of the immune system that have cytolytic activity. A distinction can be made, however, between classical and non-classical class I molecules. Class Ia, or classical, molecules are expressed at high levels on virtually all nucleated cells of the organism, with the notable exception of cells found in some immunoprivileged sites such as the central nervous system (CNS) and the placenta. Class Ia molecules are very heterogeneous within an outbred population, particularly at the level of the ligand binding groove, which is used to present short peptides of endogenous origin to CTLs via their T cell receptor (TCR). The main role of class Ia molecules is to allow CTLs to detect and destroy cells invaded by viruses or intracellular bacteria, so limiting the multiplication and spread of these pathogens. By contrast, class Ib, or non-classical, molecules are expressed at much lower levels than the classical ones, and in some cases they have more restricted tissue distributions. They are also less polymorphic, which could be explained in some cases by the fact that they associate with ligands that are much less prone to genetic variations . For example, CD1 molecules can present microbial phospholipids, H2-M3 selectively binds to peptides carrying N-formyl-methionine residues, and HLA-E and its functional homologues in other species, H2-Qa1 in the mouse and RT-BM1 in the rat, associate with sequences derived from MHC class Ia leader peptides. Whilst others have proposed that these class Ib molecules derived from convergent evolution , we have argued that they are most probably orthologues .
Class Ib molecules can be recognised by a wide array of receptors, including not only αβ TCRs but also γδ TCRs and a whole variety of inhibitory or activatory receptors present at the surface of NK cells, NKT cells and even cells of the monocyte/macrophage lineage [4, 5]. Hence, whilst class Ia molecules have a prominent role in inhibiting NK cell lysis, in line with the 'missing-self' hypothesis , class Ib molecules can have more complex roles in modulating negatively or positively the immune response .
Prominent expression of certain class Ib molecules has been found in immunoprivileged sites, e.g. HLA-G in the human placenta  or RT1-U in the rat central nervous system . The definition of an immunoprivileged site is a tissue where non-histocompatible grafts are rejected less vigorously than elsewhere, such as in the central nervous system, the anterior chamber of the eye or the placenta . During pregnancy, the foetus is tolerated by the maternal immune system despite the fact that it effectively represents a semi-allogeneic graft (and even fully allogeneic in the case of surrogacy). The expression of HLA-G in the cytotrophoblast, at the foeto-maternal interface, in place of the class Ia counterparts, HLA-A and HLA-B, has long been suspected to contribute to this induction of tolerance. Whilst the precise 'raison d'être' of HLA-G is still evasive , this molecule is clearly capable of performing functions that can either activate or inhibit immune responses. On the one hand, when expressed in transgenic mice, it can fulfil a role in antigen presentation to mouse CTLs . On the other hand, it is endowed with at least four inhibitory functions relevant to immune responses: first, it can bind directly to inhibitory receptors found on NK cells and other leukocytes [4, 5]; second, it possesses the appropriate leader peptide for binding to HLA-E, which will in turn inhibit NK cells via their CD94/NKG2 receptors ; third, as shown recently, soluble forms of HLA-G produced by placental cells induce apoptosis of activated CD8+ T cells , and fourth it can inhibit CD4+ T cell proliferation .
The fact that no obvious orthologue of HLA-G has yet been identified in rodents has made it difficult to investigate the precise role of this class Ib molecule, and somewhat tempers the notion of its potential pivotal importance in the healthy development of foetuses during pregnancy. In mice, two class Ib molecules have been suggested as potential functional homologues for HLA-G, namely T25  and Qa2 . Whilst very little data on the actual expression and function of the former have so far been produced, more and more evidence has accumulated to suggest that Qa2 molecules have multiple characteristics reminiscent of those of HLA-G, namely the capacity to activate alloreative CTLs , the existence of soluble forms , the inhibitory activity towards NK cells , and the expression in oocytes .
In rats, the expression in the placenta of transcripts encoding soluble class Ib molecules related to RT1-E u was reported earlier . Interestingly, we have found that transcripts for molecules very closely related to those identified in placenta can also be found in neurospheres , which are in vitro cultures of neural stem cells , as well as in cDNA libraries of lymphoid tissues. Altogether, these form a group of sequences that are very closely related to one another, and more distantly to the RT1-E u molecule . We have therefore chosen to name the members of this group RT1-E2, and report here on their characterisation.
Neurosphere cultures express MHC class Ib molecules
In a previous report, we described our study of the levels of MHC expression in cultures of rat neurospheres derived from the LEW inbred rat strain . At the time, we had noticed that the monoclonal antibody (mAb) MRC OX-18, which binds to the majority of rat MHC class Ia and class Ib molecules, gave more reproducible results than those obtained with F16.4.4, a mAb which stains only class Ia molecules. To investigate this variability, several experiments were carried out to compare staining obtained with either MRC OX-18 or F16.4.4 in neural stem cells, both at the time of their isolation and over time as they started growing as neurospheres in vitro. Over several experiments, we consistently observed that:
(II) In those cells recovered in neurospheres after 48 hours, staining with MHC class I-specific antibodies could consistently be detected, albeit at extremely low levels (Fig. 1A). Given that the vast majority of the cells die during the first 48 hours of culture , it is unclear whether this MHC class I expression was somehow induced, or whether the capacity of cells to survive, aggregate and proliferate correlates with a pre-existing low level of expression of MHC class I molecules at their surfaces.
(III) MHC class I expression in neurospheres rose progressively over the course of the first week, the staining obtained with MRC OX-18 appearing sooner, and being consistently higher, than that obtained with F16.4.4. In some cases, such as the one shown in panel B of Figure 1, we even obtained positive staining with MRC OX-18 and absolutely none with F16.4.4, suggesting that neural precursors initially express MHC class Ib but no class Ia molecules, the expression of the latter arising after a delay of a few hours or a few days. The differences in staining levels were not due to a difference in staining efficiency between the two mAbs since such a discrepancy was not found on cells that express MHC class Ia molecules at high levels, such as the CRNK lymphoblastoid cell line (Figure 1C). Furthermore, in separate experiments on mouse L cells stably transfected with RT1-Al, we also found that F16.4.4 always stains RT1. A molecules at least as well as MRC OX-18 (not shown).
We also noted that, after the first passage and over the course of the next two to three weeks, the MHC class I staining levels, particularly those obtained with F16.4.4, were much more difficult to reproduce between experiments, and could go up and down erratically at various stages. Attempts to correlate these variations with cell density, neurosphere size, medium composition, time after passage or feeding of cells were unsuccessful (not shown).
Cloning, classification and characterisation of RT1-E2 cDNAs
Summary of cloned RT1-E and RT1-E2 sequences identified to date
RT1 g (PC12)
PC12 cell line 
RT1 l (LEW)
RT PCR from LEW placenta
4 + 1 missing 2 nucl. at the start of exon 5
2 + 1 missing exon 7
RT PCR from LEW neurospheres
2 + 1 carrying 15 extra nucl. at start of exon 6
5 + 1 carrying 15 extra nucl. at start of exon 6
RT PCR from PVG-RT1 u neurospheres
RT1 n (BN)
BN PAC clone from RPCI-31 library
cDNA library from DA mitogen-induced lymphoblasts 
1 clone missing 2 nucl. at the start of exon 5
RT1 c (PVG)
cDNA library from PVG NK cells 
2 +1 partially spliced
Sprague Dawley (outbred)
bi281890 & bi283769 (both have 15 extra nuc. at the start of exon 5 because of mutated splice acceptor) + bi300597, aw921555 & bg372625
We decided to call the members of the 11-strong group RT1-E2 to mark their close relatedness to one another, and their relative distance from RT1-E sequences. Remarkably, the number of RT1-E2 loci is apparently quite variable among haplotypes, but at least one locus is present in every one of the six MHC haplotype we have analysed, whereas RT1-E sequences were identified in only two out of those six MHC haplotypes.
cDNAs deriving unambiguously from four separate RT1-E2 loci were identified in the RT1 l MHC haplotype carried by LEW rats, which we call RT1-E2a l , -E2b l , -E2c l and -E2d l . We decided on this nomenclature rather than calling these sequences -E2, -E3, -E4 because for some of the haplotypes where only one sequence was identified, the classification into those four loci is rather tentative since it relies on only a handful of residues within the intracellular portion of the molecule (see Table 1 and Figures 2 and 3). For example, for the RT1 g haplotype, where only three clones were obtained through RT-PCR, we cannot conclude whether the differences between these are the result of independent clones from three separate loci, or of PCR-generated artifactual hybrids between sequences derived from two or more loci.
One of the more remarkable features of these RT1-E2 molecules is the proportion of cDNA clones predicted to yield soluble MHC class I molecules, and this via three different mechanisms:
First, transcripts from the RT1-E2a l locus, which were initially identified in LEW placenta , all lack exon 5, which codes for the transmembrane domain.
Third, the other four clones obtained from this DA library were for the RT1-Eav 1molecule, and harbour a 36 nucleotide deletion in the middle of exon five, resulting in the removal of the entire stretch of hydrophobic residues that constitute the membrane spanning region.
Comparison of the large collection of RT1-E2 cDNAs that we obtained revealed two sites where alternate splicing events apparently take place, both of them in regions corresponding to the intracytoplasmic domain of the protein. Firstly, at the junction of exons 5 and 6, the addition of 15 nucleotides results in a stretch of 5 amino acids (TAFLL) in the final protein products. In RT1-E u , RT1-E2a l and RT1-E2 n this is systematic since it is due to a single nucleotide mutation at the level of the splice acceptor at the start of exon 6, which changes from the canonical AG-GTGGGA, which is found in most rodent class I genes, including RT1-A u , to AA-GTGGGA. This results in the use of another AG splice acceptor found 15 nucleotides upstream in the sequence of intron 5.
In parallel with this systematically different splicing, this exon boundary is also the site of alternate splicing since we did find that the upstream splice acceptor had been used in one of the six bona fide E2c l clone and in one of ten for E2d l , despite the presence of the canonical AG splice acceptor (which was in those two cases carried by the cDNA clones themselves). By comparison, it is probably worth mentioning that equivalent events of alternate splicing at that site have never been observed in any of the several hundred cDNAs for RT1-A molecules that we have analysed over the years.
On the other hand, exon 7 is a site where alternate splicing has repeatedly been observed for many of the classical and several non-classical rodent class I genes [28, 33]. In our case, exclusion of exon 7 was observed in one of the six E2c l clones and in clone 4.6 classified as RT1-E2d g .
Finally, out of the three clones obtained from a cDNA library prepared from PVG NK cells , and all deriving from a single RT1-E2 c locus, one was found to be only very partially spliced, starting in the middle of exon 2, and still carrying introns 2, 4 and 5 whilst the two others were correctly spliced out.
Searching with 3' untranslated region (3' UTR) sequences from the RT1-E2 cDNA clones selected from polyA-primed libraries, scored hits with five EST clone in the nucleotide database, all of which are derived from libraries assembled from mixes of various tissues obtained from outbred Sprague Dawley rats, and so are uninformative regarding the tissue distribution of RT1-E2 transcripts. These clones cover very little of the coding sequence, and sequence comparison separates them into two groups (see DNA alignment provided as supplemental data). The first, which consists of BG372625, AW121555 and BI300597, shows almost complete identity with the 3' UTR sequence predicted from the RT1-E2 n gene (see below). The sequence available for the first two members of this group stretches as far as the exon 5/6 boundary, and both clones carry the extra 15 nucleotides discussed previously, due to a mutated splice acceptor. The second group comprises the BI281890 and BI283769 clones that are slightly divergent, but more homologous to one another than to any of the other RT1-E2 sequences available to date. Because both of these sequences end very close to the stop codon, we could not conclude definitively whether they are representative of RT1-E2 or of another closely related locus type.
Expression of RT1-E2 molecules is not restricted to immunoprivileged tissues
On the basis of these multiple RT1-E2 sequences, we designed a specific primer pair (E2us-E2ex5) that could be used for the diagnostic PCR amplification of an internal portion of the cDNA from the start of exon 3 to the start of exon 5 (for more information, see M&M and DNA alignment provided as supplemental data). Using this for RT-PCR on mRNA derived from various LEW tissues, we could detect a broad distribution of RT1-E2 mRNA, albeit at lower levels than in the placenta. More specifically, RT1-E2 transcripts were as prominent in placenta as in neurospheres, readily detected in spleen, kidney and testis but much more difficult to detect in mRNA prepared from brain parenchyma (data not shown).
Cloning of the RT1-E2n gene
Searching nucleotide databases for homologues of RT1-E2 sequence only revealed one hit called "clone 4" (acc. U50447). We have previously argued  that this "clone 4", a cDNA isolated by RT-PCR from BN rat splenocytes  was probably a PCR-generated hybrid comprising the 550 nucleotide upstream part of a class Ib sequence, where it differs from the sequence previously designated as RT1-E g by only two nucleotides, and the downstream part of RT1-A1 n . The fact that "clone 4" was isolated from a BN rat suggested that the RT1 n MHC region carries at least one locus for an RT1-E2 sequence. The search for RT1-E2 sequences in the BN haplotype was greatly facilitated by the physical map published by Ioannidu and colleagues . A set of overlapping PAC clones covering the MHC class I regions likely to contain the RT1-E2 n (BN) genes was obtained from the Resource Centre of the German Human Genome Project (RZPD, Berlin, Germany) (see M&M). DNA from these PACs was digested by either BamH I or PstI, and analysed by Southern blotting with oligonucleotide E2us. This probe hybridised to the overlapping PAC clones 473K19 and 303P13, and revealed a single BamH I fragment of 4.5 kb and a single Pst I band around 2.8 kb. These bands were subcloned from the corresponding PAC and sequenced. The single sequence, which covers 4992 nucleotides, comprises the entire sequence of a class I gene, including 300 bp of its promoter and the 8-exon structure typical of most MHC class I genes (Fig. 4). Our results suggest the existence of a single RT1-E2 gene in the RT1 n MHC haplotype, and are compatible with this gene being located at the telomeric end of clone O06367, which had been labelled as RT1-E on the map published by Ioannidu and coworkers . The analysis of the entire genomic sequence of this region (acc AJ314857) confirms this finding. The hypothesis that "clone 4" is probably a PCR-generated hybrid is now further supported since the RT1-E2 n genomic sequence that we describe here matches the upstream sequence of "clone 4" perfectly, but differs from it downstream.
Comparison of promoter sequences
Whilst the sequences of these RT1-E2 promoters are relatively well conserved compared to the other MHC class I genes, several differences that are localised in the key regulatory DNA sequences can be observed. The three important elements within the core promoter are the CCAAT box, the TATA box and the initiator . The CCAAT box is conserved among the genes that we compared. Whereas H2-K b and RT1-A l contain a canonical TATA sequence, RT1-E2 shows the variant sequence TGTAA also found in RT1-C l and the mouse H2-Q6 to H2-Q9 genes (not shown here). Since MHC class I gene expression is also dynamically regulated by cytokines, we examined the three major elements within the upstream promoter region, namely the κB1 element [39, 40], the Interferon Response Element (IRE) and site α, which is crucial for routes of activation involving the transcriptional coactivator CIITA [41, 42]. All three sites were identified in the RT1-E2 promoters, but were found to diverge from those of other class I promoters by individual nucleotide differences that could presumably result in alterations of its activity. Preliminary experiments carried out with luciferase-based reporter constructs containing either the RT1-E2 n or the H2-K b promoter suggest that the RT1-E2 promoter is much weaker than that from H2-K b , but that transactivation can be boosted by the transcriptional coactivator CIITA and by NF-κB (not shown). This finding is in line with a recently published computer algorithm which predicts that the sequence of the κB1 element found in RT1-E2 would still support transactivation by NF-κB . The activity of the RT1-E2 promoter would thus still be subject to modulation by certain cytokines via CIITA and NF-κB, as described for other MHC class I genes [38, 44].
Expression of RT1-E2 in transfectants and its disruption by inhibitors
In the developing mouse brain, expression of MHC class I molecules has been reported to have a role in the establishment of neuronal connections [46, 47]. These studies also showed that different mouse MHC class I H2 loci displayed different well-defined spatio-temporal patterns of expression. A similar finding was also recorded for RT1-U, a rat class Ib molecule . Whilst our finding of RT1-E2 expression in neural precursors does not prove its direct involvement in the establishment of the CNS architecture, this possibility will be worth investigating further.
Several other reports have also suggested that expression of MHC class I molecules is generally low in cultures of proliferating stem cells of various origins [48–51], with some of these reports pointing to a preferential expression of class Ib molecules comparable to our observations with rat neurospheres [50, 51]. In all these cases, however, it may be that the expression of class Ib molecules themselves may not be upregulated, but becomes more noticeable when that of their class Ia counterparts is selectively repressed.
The preferential expression of certain non-classical class I molecules could be related to the observation that stem cells are relatively insensitive to lysis by NK cells despite their lack of class Ia molecules in certain systems . The fact that grafted stem cells can induce tolerance to secondary allografts  could also be related to their expression of class Ib MHC molecules endowed with immunoregulatory functions. In other instances, however, the activity of NK cells can clearly play a very important role in limiting the efficiency of grafted cells survival .
It is possible that RT1-E2 molecules could serve as markers of neural progenitor cells that proliferate, or have the potential to do so. If this were the case, RT1-E2 could be a useful extracellular marker to enrich for rat neural stem cells. This, however, will require the generation of RT1-E2 specific monoclonal antibodies. Given the striking level of inter-strain conservation of the extracellular domain of these molecules, and the fact that we have found RT1-E2 loci in all six of the rat MHC haplotypes we have studied, it is probably not surprising that we have so far failed to find RT1-E2-specific mAbs amongst the antibody panels raised using conventional rat anti-rat immunisations. Similar difficulties have been encountered in the past for other MHC class Ib molecules, against which alloreactive responses are weak or absent on account of the level of sequence conservation, while xenoreactive antibodies tend to cross-react broadly on many class Ia and Ib molecules. To overcome this hurdle, access to a transgenic mouse expressing a rat class Ia molecule constitutively would be extremely useful for immunisation purposes.
Comparison of RT1-E2 sequences with known mouse class I genes has not revealed any obvious orthologue. On the other hand, the H2-Qa2 family of mouse class Ib genes does share several features with RT1-E2 that suggest they may be related both genetically and functionally. Firstly, the Q6 to Q9 loci, which encode H2-Qa2 molecules, lie in the region of the mouse MHC bordered by the framework markers Bat1 proximally and Pou5f1 (Oct3/4) distally . This interval corresponds to the RT1-E/C region in the rat within which we have mapped the RT1-E2 locus of the BN rat. As for RT1-E2, the number of loci for H2-Qa2 is variable between mouse strains, with up to six functional loci found in the H2 bc haplotype , all of which are highly homologous to one another, and some of which give rise to soluble molecules.
One factor that has greatly facilitated the study of Qa2 antigens is the fact that certain mouse haplotypes, e.g. H2 k , lack a functional Qa2 locus. Whilst this has allowed the development of Qa2-specific alloreactive mAbs, it also argues against a pivotal, indispensable, role of Qa2 molecules in the vital physiological processes of mice. But this is of no great surprise since even mice made deficient for the β2-microglobulin gene remain viable, despite the alteration reported in their neuronal connectivity . Another point worth mentioning is that certain strains of β2-microglobulin KO mice breed very inefficiently , and that loci affecting fertility have been described and mapped to the region of the rat MHC where RT1-E2 genes are found . It is therefore conceivable that these observations could turn out to correlate with the expression of these class Ib molecules in the placenta.
Another recently uncovered property of H2-Qa2 molecules is their capacity to inhibit killing by NK cells . Many inhibitory and activatory NK receptors have already been uncovered in the rat, and genes carried in the RT1-E/C region of the MHC are known to regulate the activity of alloreactive NK cells [61, 62]. Regarding the capacity of MHC molecules to regulate NK activity negatively via their CD94/NKG2A receptors, there exists a very particular dichotomy at the level of the initiation of translation of the murine class I proteins: some proteins start with MGAMAPRTLLL, whilst in many others, including most H2-K alleles, the codon for the first methionine residue is mutated and translation therefore starts at the second one. In the latter case, it has been clearly shown that the MAPRTLLL or MAPRTLLLL peptides resulting were unable to bind significantly to H2-Qa1 , and translation of those class I molecules would therefore not result in the inhibition of NK cells via their CD94/NKG2A receptors. Whilst all rat class Ia sequences where this sequence is known to start with the first methionine, this first ATG is not present in many rat class Ib molecules including RT1-Cl  and RT1-Eu , and the second methionine is therefore used. Since both RT1-Cl and RT1-Eu have been shown to be potential activators of allorecognition by NK cells [62, 64], there is therefore a correlation whereby the molecules that can inhibit NK cell activity also have a functional codon for the first methionine, and the ones that can be recognised by NK activatory receptors do not. In this regard, RT1-E2 molecules do harbour a functional codon for the upstream methionine, and one might therefore be tempted to predict that their propensity would be more towards inhibiting NK cells. If this turns out to be true, this will be a further point of similarity with H2-Qa2 and HLA-G both of which have a potential role in inhibiting NK cell activity.
Over the past few years, MHC class Ib molecules have been the focus of an ever-increasing amount of interest, related to their multiple roles in antigen presentation and immunoregulation, particularly of NK cell activity. Because of their lower levels of expression and limited heterogeneity, class Ib molecules have proven more difficult to study than their classical class Ia counterparts, especially because of the paucity of serological reagents. In mice and rats, this is further complicated by the fact that the MHC region carries many more class Ib loci than does the human MHC. Unravelling the function and evolutionary history of rodent class Ib molecules will require progressive characterisation of these various loci, and the results described here should contribute significantly towards this end.
Unless otherwise specified, all chemicals were obtained from Sigma, France.
Rats were bred and maintained in specific pathogen-free conditions at either the Babraham Institute (Cambridge, UK) or the "Service d' Animalerie de l'IFR 30", Hôpital Purpan, Toulouse, France.
Cell culture and chemicals
Neurosphere cell cultures were performed as previously described . Briefly, cells were prepared from the striatum of embryonic (E14) LEW rats. Following trituration, cells were plated in growth medium containing HAM's F-12/DMEM (1:3, Life Technologies) supplemented with PSF antibiotic mix (1%, Life Technologies), EGF (20 ng/ml, R&D Systems), FGF-2 (20 ng/ml, R&D Systems) and N2 supplement (1%, Life Technologies), and passaged every 7 days after trituration to a single cell suspension.
The mouse L (tk-) and simian COS-7 cells were grown in DMEM (Life Technologies) supplemented with 10% fetal calf serum (FCS) and passaged twice a week by trypsinization. For the analysis of the recovery of MHC class I expression after acid wash, cells were collected, washed twice in PBS and incubated for 1 min in 1 ml of 300 mM glycine-NaOH (pH 3.0) supplemented with 1% BSA, before being washed once in growth medium. The cells were then returned to 37°C for 6 h either in growth medium alone, or in the presence of inhibitors. MG-101 and MG-132 were stored in DMSO and used at 250 μM. Brefeldin A, monensin were stored in EtOH and used at a final concentration of 5 μM.
Monoclonal antibodies (mAbs)
The mouse mAb MRC OX-18  recognises an epitope in the α3 domain of most rat MHC class Ia and Ib molecules, whilst F16.4.4  recognises only class Ia molecules. Supernatants from hybridomas 11-4-1  and B22-249  were used to label H2-Kk and H2-Db, respectively. All four primary antibodies were used at saturating concentrations. For MRC OX-18, F16.4.4 and B22-249, absolutely no cross-reactivity was detectable on mock transfected COS 7 or L(tk-) cells.
The oligonucleotides used had the following sequences:
EJ 001: 5'-GCTCTAGAGTCCAGGCAGCTGTCTTCA-3'
EJ 002: 5'-TGCTGCTGGCGGCCGCCCTGG-3'
380: 5'-CCTCCCTCCG/ACCAACTCCAACACGGGAATGTCTGTTATTC TTGGAACTGTG-3'
The respective positions corresponding to these is indicated on the nucleotide alignment provided as supplementary data.
λgt10 forward: 5'-AGCAAGTTCAGCCTGGTTAAG-3'
λgt10 reverse: 5'-CTTATGAGTATTTCTTCCAGGGTA-3'
Cloning of class I sequences by RT-PCR
This was performed as previously described . Briefly, mRNA was purified from either neurospheres or E17 rat placentas. After oligo dT-primed reverse transcription, PCR was carried out between oligonucleotides EJ 001 and EJ 002 before cloning into the pCMU-Db plasmid via Not I and Xba I sites. Plasmid DNA from recombinant colonies was subsequently analysed by restriction digestion with BamH I and Pvu II, and positive clones were sequenced using the Big Dye Terminator method (Applied Biosystems).
Cloning of RT1-E2 cDNAs from cDNA libraries
Two cDNA libraries were screened.
1) a PVG NK cell library prepared in the eukaryotic expression vector pMet7  was amplified in E. coli DH10B (Life Technologies). 0.5 × 106 colonies were plated out, grown overnight at 37°C, and duplicate lifts made on to nylon-reinforced cellulose nitrate Opticron BA-S 83 0.2 uM membranes (Schleicher & Schuell). The membranes were denatured and neutralised by standard procedures, dried and baked at 80°C in a vacuum oven for 2 h. The membranes were hybridised with γ32P labelled oligonucleotide 380 in Puregene Hyb-9 Hybridisation solution (Flowgen) at 60°C overnight. After several washes over 2 h in 2x SSC at 50°C, the membranes were exposed to XR film (Fuji) for various periods of time. Three duplicated positive spots were selected and subjected to three rounds of purifying selection with oligonucleotide 380 to obtain single, positive colonies.
2) 2 × 105 bacteriophages from a λgt10 cDNA library prepared from DA rat lymphoblasts  were plated out on C600 E. coli, grown for 8 h at 37°C then stored overnight at 4°C. Duplicate lifts were made on to Opticron 83 or Hybond N+ (Amersham Pharmacia Biotech) membranes, which were then treated and hybridised to oligonucleotide 380 as described for the PVG cDNA library. Five positive clones were subjected to three rounds of purifying selection by hybridisation with oligonucleotide 380 to give pure phage stocks. Phage DNA was obtained by PCR using PWO DNA polymerase (Roche) and λgt10 forward and reverse primers, using an annealing temperature of 55°C. The products were cloned into pSTBlue1 (Novagen) and sequenced.
Sequences were edited using GCG software (University of Wisconsin Genetics Computer Group, Madison, WI); FASTA and BLAST were used for comparisons with sequences in the online nucleotide databases. Database accession numbers for the novel or published sequences mentioned are given in the legends to Figures 2 and 5.
Trees for comparison of sequences were obtained using Puzzle , starting from the file of aligned protein sequences shown on Fig. 2. One thousand quartet puzzling steps were used, and support values are indicated for each branch. Trees were visualized using the "Treeview" freeware package written by Roderic D. M. Page (available on http://taxonomy.zoology.gla.ac.uk/rod/treeview.html).
Genomic mapping and sequencing
A set of overlapping PAC clones derived from the RPCI-31 library , and expected to cover the MHC class I region containing the RT1-E2 n gene, was purchased from the Resource Centre of the German Human Genome Project (RZPD, Berlin, Germany). From proximal to distal, these are: RPCI-31 473K19, 303P13, 31C24, 374E16, 100B16, 571A22, 583F5, 460D3 and 462P8. PAC DNA was isolated using a standard alkaline lysis "miniprep" protocol. DNA from these PAC was then digested either by BamH I or Pst I. After agarose gel electrophoresis, DNA was transferred onto Hybond-N+ membranes (Amersham Pharmacia Biotech) by passive transfer in 1.5 M NaCl and 0.5 N NaOH. Hybridization was performed overnight with the radiolabelled oligonucleotide probe E2us at 37°C in 5x SSC, 12.5x Denhardt reagent, 0.5 M sodium phosphate (pH 7.0), 2% (w/v) SDS, 0.1 mg/ml sonicated salmon sperm DNA. Washing conditions were: 30 min at 37°C in 2x SSC, 0.1% SDS followed by twice 30 min at 50 °C in 2x SSC, 0.1% SDS. After washing, Kodak X-OMAT AR5 films were exposed to membranes for various times.
The pN3-ICP47 plasmid was constructed by cloning a Not I/Hind III fragment obtained from the pCDNAI-ICP47  into the pEGFP-N3 vector (Clontech). The pMCS-Gtx-EGFP was created by cloning the Gtx IRES  between the Kpn I and the BamH I sites of the pEGFP-N1 vector (Clontech). The pDb-Gtx-EGFP and the pE2u-Gtx-EGFP plasmids were then constructed by blunt-end cloning the H2-Db and the RT1-E2u cDNA obtained respectively from the pCMU-Db and the pCMU-E2u vectors into the Xho I (blunt-end) site of pMCS-Gtx-EGFP.
Stable transfection of L(tk-) cells with pCMU-Db or pCMU-E2u was performed by the calcium phosphate method using pSV2neo as a co-transfection marker . Transfectants were then selected using 0.5 mg/ml G418 (Life Technologies), and cells expressing the appropriate MHC molecule were selected by flow cytometry, using MRC OX-18 for RT1-E2u, and B22-249 for H2-Db. After selection, the cells retained similar levels of auto-fluorescence. Transient transfections of COS-7 cells were performed as previously described .
Staining of cells for flow cytometry
Cells were trypsinized and washed once in culture medium before incubation with primary antibody on ice for 30 min. The cells were then washed three times by centrifugation using cold PFN (PBS containing 2% (v/v) FCS and 0.1 % (w/v) sodium azide) before incubation with secondary antibody (fluorescein isothiocyanate [FITC]-conjugated rabbit-anti-rat IgG diluted 1/100 in PFN) for 30 min on ice. After two washes in PFN, cells were resuspended in PFN containing 2 μg/ml propidium iodide and analysed on a FACScan (Becton Dickinson, San Jose, USA) within an hour of staining. For cell sorting, PFN was replaced by sterile tissue culture medium, and an Epics ALTRA (Beckman Coulter) was used.
We thank Claudie Offer for excellent DNA sequencing assistance, Drs. V. Mauro, N. Shastri for kindly providing plasmids, and Philippe Le Bouteiller for his helpful advice on the manuscript, as well as the three anonymous referee's whose suggestions contributed very significantly to the editing of this manuscript. P.L. was supported by the French Polynesia Government, C.A. by the Ipsen Foundation and ARSEP, and E.J. by INSERM. The Functional Immunogenetics Laboratory at the Babraham Institute is supported by Core (Competitive Strategic Grant) funds from the UK BBSRC.
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