- Research article
- Open Access
Prevalence and associated factors of treatment failure among HIV/AIDS patients on HAART attending University of Gondar Referral Hospital Northwest Ethiopia
© The Author(s). 2018
- Received: 27 July 2018
- Accepted: 30 November 2018
- Published: 17 December 2018
The initiation of highly active antiretroviral therapy (HAART) plays a significant role in the clinical management of HIV infected people by preventing morbidity and mortality. This benefit becomes, the most terrible when treatment failure develops. Thus, this research aims to assess the prevalence and associated factors of treatment failure among HIV/AIDS patients on HAART attending University of Gondar Referral Hospital Northwest Ethiopia.
Patients on ART with a minimum of 6 months and up to 12 years of treatment were being enrolled. The prevalence of treatment failure, immunological failure and virological failure among people living with HIV/AIDS attending University of Gondar referral hospital were 20.3, 13.2, and 14.7%, respectively. Patients who had no formal education (Adjusted odds ratio (AOR): 3.8; 95% CI, 1.05–13.77), primary level education (AOR: 4.2; 95% CI, 1.16–15.01) and duration on ART < 6 years (AOR: 2.1; 95%CI, 1.12–3.81) were a significant risk factor. However, initial adult regimen D4T + 3TC+ EFV (AOR: 0.025; 95% CI, 0.002–0.36), AZT +3TC + NVP (AOR: 0.07; 95% CI, 0.01–0.71), AZT + 3TC + EFV (AOR: 0.046; 95% CI, 0.004–0.57) andTDF+3TC + EFV (AOR: 0.04; 95% CI, 0.004–0.46) were significantly protective for treatment failure.
Timely and early identification of associated factors and monitoring antiretroviral therapy treatment failure should be done to enhance the benefit and to prevent further complication of the patients. It is preferable to initiate ART using any one of the following ART regimens: AZT +3TC + NVP, AZT + 3TC + EFV and TDF + 3TC + EFV to prevent treatment failure. Since the prevalence of this treatment failure and its associated factor may be different from other ART centers and community in Ethiopia, further national representative institutional based cross-sectional researches are needed across all ART centers of Ethiopia in order to determine the prevalence of treatment failure and its associated factors.
- Treatment failure
- Immunological failure
- Virological failure
Human immunodeficiency virus (HIV) has affected all parts of the world. According to the 2017 UNAIDS report, 36.7 million people were living with HIV in the world. In the same year, one million people died from acquired immunodeficiency syndrome (AIDS) related illnesses and 1.8 million were new HIV infected people . Sub-Saharan Africa is a region highly affected by HIV epidemic. Ethiopia is one of the Sub-Saharan African countries with the highest numbers of people affected by the problem [2, 3]. In Ethiopia, there were 710,000 patients infected with HIV/AIDS in 2016. Around 404,405 HIV patients were on an Antiretroviral therapy (ART) and around 20,000 AIDS-related deaths were reported in the same year . In Amhara regional state, where this research carried out, 191,067 people were living with HIV/AIDS in 2015 .
Even though, lack of remedial therapy for HIV/AIDS, the initiation of ART played an important role in the clinical management of peoples infected with of HIV/AIDS . But a significant number of patients fail to achieve a sustained virological and immunological response to treatment among the HIV-infected patients receiving highly active antiretroviral therapy (HAART) .
Antiretroviral (ARV) treatment failure is defined as progression of disease and high risk of mortality after beginning of HAART. It can be assessed by clinical failure (occurrence of new infections (OI) or malignancy, symptomatic of clinical disease progression; recurrence of previous OI, onset, or recurrence of WHO stage IV (& certain stage III) conditions, etc.), immunologic failure (a decline in the CD4+ T cell count), or virological failure (The inability to maintain suppression of viral replication to an HIV RNA level < 1000) either in combination or discordantly. Clinical and immunologic criteria have been used for assessing treatment failure in the absence of viral load (VL) test [7–10]. If VL test is available, VL determination and guiding of ART treatment by the gold standard test (VL test) should be recommended . The discordant immune response in patients those doesn’t show a significant increase in the CD4+ T cell count despite viral suppression may also asses with VL and immunological criteria [12, 13].
The patients who had failed ARV therapy treatment are attributed to the higher number of side effects and have the greatest likelihood of experiencing drug resistance and treatment fatigue as a result of being on treatment longer . Development of drug-resistant virus strains (restricting ART alternatives) can be an additional threat if this virus begins to transmit in the population [15, 16]. Therefore, early detection of treatment failure is crucial to sustaining the success of the treatment [17, 18]. Studies conducted in East Africa have revealed that a high prevalence of immunologic failure ranging from 11 to 57% among clients on HAART; moreover, the extent increases as the time of follow-up increases [19–21]. The immunological failure rate in Ethiopia was also found to be high .
The timing and accuracy of identifying the risk factors associated with treatment failure include socio-demographic factors (e.g., sex, marital status, educational level, etc.) and clinical factors (e.g., adherence, WHO stage, drug toxicity, ART regimen, CD4+ T cell count, etc.) [23–25] helps to describe timely predictors of treatment efficacy that permit better use of drugs, to avoid unnecessary side effects of ART drug and prevent drug resistance new strain viruses. Identification of risk factors also decrease the economic weight due to a magnificence cost of ART drug and will help as a guide for health professionals and higher officials to alleviate the problem and to develop strategies toward the decreasing rate of treatment failure.
In Ethiopia, viral load determination and guiding ART treatment with the gold standard test (viral load) has started recently because of resource limitation. Consequently, there is limited data on treatment failure and its associated risk factor. The available studies [26, 27] were conducted on first-line antiretroviral treatment failure. These studies didn’t address second-line and immunovirological discordance of treatment failure.
Ethiopia started to implement an ambitious plan launched by UNAIDS in 2014 known as “90–90-90”. This will focus to achieve, at least 73% of all HIV patients will be virally suppressed in 2020 and to end the AIDS epidemic by 2030. The 90–90-90 ambition plan intends to, by 2020; 90% infected individuals with HIV will be diagnosed, 90% diagnosed patients will be receiving ART drug and the viral load in 90% of patients on ART will be undetectable . To achieve this goal, data on immunological and virological treatment failure is required.
Furthermore, the Ethiopia national treatment program needs data to aid the realizing high impact and targeted prevention program; to achive successful elimination of mother to child transmission and improving sustainable quality care and management. Thus, this research was carried out to offer data on prevalence and associated factors of treatment failure among HIV/AIDS patients on HAART attending University of Gondar Referral Hospital Northwest Ethiopia.
Study area, design, period and population
A hospital based cross-sectional study and retrospective record review was conducted from February to April 2017. The study was conducted at University of Gondar Referral Hospital which is found at Gondar town, the town is located 747 km from the capital city of the country, Addis Ababa and 182 km far from Bahir Dar which is the capital city of Amhara regional state. According to the recent administration, the town has 12 sub cities which consist of 21 kebeles. Gondar is one of the ancient and densely populated towns in Ethiopia. Based on the figure from the central statistical agency in 2008, Gondar has an estimated population of more than 206,987 (98,085 males and 108,902 females) . In Gondar town, there is one teaching referral hospital providing specialized clinical services, 8 health centers, and 15 private clinics serving the population. Currently 13,753 HIV patients and 5389 on ART patients are attending at the University of Gondar Referral Hospital.
All people living with HIV/AIDS (PLWHIV) and those enrolled in UOG Referral Hospital ART laboratory was the source population. Adult HIV patients who had received ART for more than 6 months, who visit UOG referral hospital ART laboratory during the study period and who consented to be involved in the study was the study population.
All adult people living with HIV and those had had base line CD4 + T cell count, at least 6 months follow up duration of ART and those patients willing to give blood for VL and CD4+ T cell count were included in the study.
Patients who had been seriously sick, unable to give information, incomplete data specially laboratory and clinical data such as:- base line CD4+ T count, base line adherence, base line drug regimen, HIV/AIDS WHO stage, weight, etc., insufficient sample and who were less than 18 years during the study period were excluded from study.
Operational definition of terms
Base line data
The data before ART initiation.
Fall of follow-up CD4 count to baseline (or CD4 falls below baseline), or CD4 levels persisting below 100 cells/mm3, or 50% fall from on-treatment peak value.
Plasma viral load greater than 1000 copies/ ml.
Which is sub divided as immunological non-responders (VL >150copies/ml and CD4+ cells increase ≥50/μl) or (VL <150copies/ml and CD4+ cells increase < 50 cells/μl), in comparison with baseline values.
Sample size determination and sampling technique
Based on single population formula and systematic random sampling technique with the following assumption, P = population proportion (estimated prevalence) = 0.5, precision, 0.05, by assuming 95% confidence interval α = 0.05 and z(1-a/2) = 1.96d was used for sample size determination. Tree hundred eghity four (n = 384) was the minimum sample size. By adding up 10% non-response rate 423 participants were involved.
Socio-demography and potential risk factors data not documented on chart (Alcohol intake, chewing khat, cigarette smoking, etc.) were collected with interview from each study participant using structured questionnaires and relevant clinical features (weight, WHO clinical stage, base line CD4 + T cell count and other consecutive CD4 + T cell count after ART initiation, ART regimen, adherence and duration of HAART, initial regimen, types of co- infections, adverse effect, reason of switching drug) of the patients were retrieved from charts (medical records) by trained nurses working on ART clinic using data extraction checklist. Blood for both CD4 and viral load tests were collected by the laboratory staff at sample collection site.
Sample collection and transport
Following standard operational procedure (SOP) sample collection and transport was done. About 10 ml venous blood was collected from each patient for CD4 and Viral load tests on the same venipuncture. For both tests 3 -5 ml of whole blood was drawn from each participant using vacutainer tube separately in two tube containing anticoagulant ethylene diamine tetra-acetic acid (EDTA). After centrifugation (3000 rpm for 20 min) plasma was separated and aliquots prepared for viral load testing. Specimens collected were labeled with ART number, study IDs and date and transported directly to the laboratory for CD4+ T cell count and to the molecular laboratory for VL test. The storage temperature for CD4+ T cell count specimen was at room temperature (20 °C – 25 °C) and tested within 24 h. For viral load testing, plasma was separated within 5 h and two aliquots of cryo-vials (with capacity of 1 ml each) prepared for transportation. Specimen transportation was on dry ice and stored at -80 °C until the test is done. Centrifugation, pipetting and aliquoting were performed following standard protocol and laboratory bio-safety precautions both at collection and testing site.
Laboratory testing methods
Following SOP quantification of absolute counts of CD4+ T cell on whole blood specimen was done using the FACS Calibur flow cytometer (BD, CA, America) in the laboratory. CD4 + T cells count was done by adding 50 μl whole blood to a reagent tube containing 20 μl of monoclonal antibodies followed by vortexing and incubation for 30 min under dark condition.
Plasma viral load was measured using Quantitative Real-Time PCR HIV-1 assay by the COBAS® AmpliPrep instrument (Roch, Homburg, Germany). Plasma was prepared from 5 ml of blood by centrifuge at 3000 rpm for 20 min.
Data processing and analysis
Data were checked every day for its completeness, edited, cleaned and analyzed using SPSS version 21. Analysis was performed using univariate and multivariate logistic regression to determine the extent of the risk factors associated with ART treatment failure. The independent explanatory variable (s) of the dependent variable was selected at univariate analysis and included in multivariate analysis with p-value < 0.2. The final association was prepared using multivariate logistic regression. P-value < 0.05 was considered significant. After organizing the result in the form of frequencies and percentages, the data were summarized and described using text and tables.
Ethical clearance was obtained from the University of Gondar, School of Biomedical and Laboratory Sciences Ethical Review Committee and official letter was submitted to the University of Gondar Referral Hospital administration prior to data collection. Written informed consent was obtained from each study participants after explaining the purpose and objective of the study. Patients who were not willing to participate in the study were not forced to participate. All the data and samples obtained from them were kept confidential by using codes instead of any personal identifiers and meant only for the purpose of the study. The laboratory results from the study participants were communicated to their physicians for appropriate management.
Socio-demographic characteristics of patients
Socio-demographic and Clinical characteristics of HIV/AIDs patients in the University of Gondar referral hospital, 2017
> = 50
Duration of ART in year
Mean ART duration in year
7 year (+ 3)
Base line WHO stage
WHO stage I
WHO stage II
WHO stage III
WHO stage IV
WHO stage during data collection
WHO stage I
WHO stage II
WHO stage III
Type of Opportunistic infection
D4T + 3TC + NVP
D4T + 3TC+ EFV
AZT +3TC + NVP
AZT + 3TC + EFV
TDF + 3TC + EFV
TDF + 3TC + NVP
D4T + 3TC + NVP
Pediatric 4C (AZT + 3TC + NVP)
To 1st line drug
To 2nd line drug
AZT +3TC + NVP
AZT + 3TC + EFV
TDF + 3TC + NVP
TDF + 3TC + EFV
ABC + ddl + LPV/R
TDF + ddl + IPV/R
Reason of switching drug
ARV drug ADH at base line
ARV drug ADH During data collection
Base line CD4 count
> = 500
CD4 count during data collection
> = 500
Viral load count
> = 1000
Component of immunological failure
CD4 Falling More Than 50%
CD4 Falling Below Baseline
CD4 Persistently Below 100
Clinical characteristics of HIV/AIDS patients
The study patients were on ART with a minimum of 6 months up to 12 years with an average time of 7 (± 3) years. Before ART initiation majority of patients had WHO clinical stage III and IV 269 (63.6%), CD4 + T cell count < 200 cells/mm3 267 (63.1%), good adherence 408 (96.5%) and on AZT +3TC + NVP 156 (36.9%) regimen followed by TDF + 3TC + EFV 87 (20.6%).
While at the time of data collection 420 (99.1%) had WHO clinical stage I and II, 44 (10.4%) had CD4 + T count < 200 cells /mm3, 420 (99.3%) had good adherence and 162 (38.2%) switched to either first line 150 (35.5%) or second line regimen 12 (2.8%). The most common reason for switching was toxicity 109 (67.3%) followed by TB 18 (11.1%). The common opportunistic infections observed during their ART follow up was TB 89 (21%) (Table 1).
Treatment failure and associated factors
Out of 423 ART patients, 86 (20.3%) had either immunological or virological failure, 56(13.2%) had an immunological failure, 62 (14.7%) virological failure and 20 (7.6%) had both immunological and virological failure and 54 (12.8%) was discordance.
Immunological failure and associated factors
Participants were followed for different periods and the total person-time of follow up was 3026 patient-years of follow up. Hence, the rate of immunological failure was 1.85% patient years of follow up. The mean plasma viral load level was 6906 copies/ml (range 0–298,869 copies/ml). Among all the study participants, 56 (13.2%) of patients CD4+ T cell counts during follow-up were below baseline CD4+ T cell count or CD4 levels persisting below 100 cells/mm3, or 50% fall from on-treatment peak value which indicates immunological treatment failure.
Bivariate and multivariate analysis of risk factors for immunological failure on ART/AIDs patients attending University of Gondar Referral Hospital 2017
> = 50
Duration on ART in year
D4T + 3TC+ EFV
AZT +3TC + NVP
AZT + 3TC + EFV
TDF + 3TC + EFV
TDF + 3TC + NVP
D4T + 3TC + NVP
AZT+ 3TC + NVP(4c)
D4T + 3TC + NVP(4a)
> = 20
Virological failure and associated factors
Participants were followed for different period and the total person-time of follow up was 3026 patient-years of follow up. Hence, the rate of virological failure was 2% patient years of follow up. The mean plasma viral load level was 6905.934 copies/ml (range 0–298,869.00 copies/ml). Among all the study participants, 62 (14.7%) of patients were found to have viral load count of > 1000 copies/ml which indicates virological treatment failure.
Bivariate and multivariate analysis of associated risk factors of virological failure on ART/AIDs patients attending University of Gondar Referral Hospital 2017
> = 50
Reason of switching
Tb + pregnant
Default + age
Current CD4 count
> = 500
Bivariate and multivariate analysis of immuno-virologic discordant on ART/AIDs patients attending University of Gondar Referral Hospital 2017
Immune-virological failure discordant
> = 50
Duration of ART in yrs.
Reason of switching drug
Associated factors for treatment failure
Bivariate vs multivariate analysis of treatment failure on adult HIV/AIDS patients attending University of Gondar Referral Hospital 2017
> = 50
No formal education
Duration of ART in yrs.
D4T + 3TC + NVP
D4T + 3TC+ EFV
AZT +3TC + NVP
AZT + 3TC + EFV
TDF + 3TC + EFV
TDF + 3TC + NVP
D4T + 3TC + NVP(4a) child regimen
AZT + 3TC + NVP(4c) child regimen
AZT +3TC + NVP
Identifying and managing treatment failure are a basic challenge for national treatment program. Sustainable treatment failure is related to difficulty to delivering quality care, the emergence of drug resistant viruses which limits the treatment option and increases the threat of morbidity and mortality. Thus the study was intended to assess the prevalence and associated factors of treatment failure among HIV/AIDS patients on HAART.
The prevalence of treatment failure as a result of immunological failure 56 (13.2%) identified in this study was similar to those reported by other studies done in Bahir Dar  and SNNP region, Ethiopia  and Uganda  showing an immunological failure of 15.9, 11.5 and 11% respectively. However; our result was slightly lower than the study done in Bahir Dar Ethiopia. The difference might be as a result of the fact that the clients (patients) of the study participated in Bahir Dar were only under the first line HAART regimens, but our study patients were either under first or second- line of HAART regimen. Since most failures occur soon after the switch is made from the first line to a second-line therapy, a shorter follow-up period is most likely to find a higher probability of failure when compared to a study with a longer follow-up period.
Our finding showed that the duration of follow up on ART < 6 years (P = 0.023) was significantly associated with immunological failure and it was 2 times more likely to cause immunological failure than the duration of follow up on ART > 6 years. This finding was inconsistent with a study done in French . The discrepancy might be due to lack of assessment of new strain ART drug resistance viruses and its prevalence rate. If the duration of follow up on ART increases (late after the introduction of HAART drug in 2005 in Ethiopia), the emergence of some new HIV drug resistance (HIVDR) strain is predictable as a result of HIV’s error-prone replication, mutation rate and viral recombination. If this new strain HIVDR virus infects others, treatment failure may be occurring and management may not be effective. So patients with short duration of follow up (< 6 year) on HAART has had two times the chance of acquiring the new strain drug resistant virus either during the duration of treatment follow up or initially by transmission than those patients infected with HIV (less likely drug resistant virus) before or early the introduction of ART even though those have had long duration of follow up (> 6 year) on ART to acquire this new strain drug resistant virus. So the high prevalence of drug resistant virus with immunological failure through reduction of CD4+ T cell count is expected in patients those ages is < 6 years than those have had > 6 years of follow up on ART.
Viral load ≥20 (P = 0.001) was the other significant factor for immunological treatment failure. This finding was consistent with other studies done in South Africa  and Eurosids  those showed viral load had the greatest impact on the CD4+ T cell decline. For patients with high viral load (more viruses) may trap more CD4+ cells in lymphoid tissue, which results in a low CD4+ cell count in blood .
The prevalence of treatment failure due to virological failure in our research was 14.7% (64/423) among the study participants. This finding was in agreement with a research done in Zambia  (11.7%), Tanzania 12.3% , Malawi 9.2% , Nigeria 13.7% , South Africa 13.7%  and Uganda 11.3% . However; our finding was lower than the other research done in Cameron 23.2  and coastal Kenya 24%  and slightly higher than the study conducted Uganda 9.9% , Bahir Dar 10.7%  and Gondar 4.1% . The observed variation may be as a aresult of difference in geographical area, the study design, the endpoint of virological failure and sample size.
Our finding showed that CD4 + T cell count during data collection < 200 cells/mm3 (AOR =10.09 (2.47–41.29), p = 0.001) were 10 times more likely to have virological failure contrasted with comparative group ≥500 cells/mm3 it was in line with the research done in Peru  and Kenya . Since the diminishing of HIV-1-specific CD4 T helper cell, as a result of decline in CD4 T cell counts, qualitative impairments of CD4 T cell function and functional impairment of CD4 T cell indirectly lead to a functional impairment of HIV-1specific CD8 T cells  or B cell responses to control viral replication . So the amount of viruses in the body increased.
In this research reason of switching as a result of default and age (AOR = 7.20 (1.99–25.94), p = 0.003) was 7 times more likely to have virological failure contrasted with the comparative group toxicity. It is inconsistent with studies done in Uganda  and Nigeria  those showed TB, breastfeeding, and pregnancy was significant for virological non-suppressed. This difference might be due to 9/15 (60%) of switching as a result of default and age in our study were within the age range of 18–29, with 53.3 virological failures. Those aged < 30 years at the time of starting ART were more likely to break off the regimen  results in a significant rise of viral load.
Antiretroviral therapy treatment failure with immune-virologic discordance in our research was 54 (13%). This finding was concordant with other research done in India that showed 28 (13.6%) discordant patients . However, a research carried out in Nigeria  showed 33% immune-virologic discordance was inconsistent with our finding. This disagreement is most likely due to the difference in sample size (206 study subject) and study design (cohort study) that applied in Nigeria.
Our findings showed that only switching of drugs as a result of toxicity (AOR = 11 (2.15–56.09), p = 0.004) was significant factors for discordant results it is in line with other studies done from the computerized database retrospectively [51, 52]. The potential causes might be marrow-suppressive medications and infiltrative bone marrow processes as a result of toxicity of known marrow suppressive drugs used in HIV-infected patients comprise zidovudine (Retrovir) and zidovudine-containing fixed combination pills that effect poor CD4 cell count responses in the setting of persistent virologic suppression and Toxicity as a result of TDF that causes the occurrence of bone fractures or changes in fat distribution this may also affect CD4 lineage .
Our study showed that the educational level was significantly associated with treatment failure. This finding was inconsistent with the research done in Addis Ababa, Ethiopia  and Bahir Dar, Ethiopia . The difference may be due to the study participant awareness for treatment failure, for instance, the study participant in Addis Ababa were all from Urban but our study subject was comprised 79% urban and 21% rural with 23% no formal educational level.
Even though the regimen containing AZT + NVP can induce high incidence of the bone marrow suppression with encouraging high rate of lukimia, the goals of ART are actually the qualitative and quantitative immunological reconstitution and the maximum possible reduction of the viral load in the longest possible time [1, 55]. In line with that, the results of this research showed that initial adult regimens such as D4T + 3TC+ EFV (P = 0.007), AZT +3TC + NVP (P = 0.025), AZT + 3TC + EFV (P = 0.016), TDF + 3TC + EFV (p = 0.009) were significantly protective for treatment failure as compared to regimen AZT + 3TC + NVP. This finding was in line with the study done in Ethiopia .
Duration of follow up on ART ≤6 years (p = 0.021) was significantly associated with treatment failure and was 2 times more likely to cause treatment failure than the duration of follow up on ART > 6 years this is in line with the study conducted in the Malaysian state of Selangor . This finding was not in line with research done in Bahir Dar, Ethiopia  Kumasi, Ghana . This is most probably due to the lake of initial viral load measurement and increment of the transmission of the drug-resistant viruses.
Acquired drug resistance testing was not done, because the testing facility has not been so far available in the country. The patient’s clinical data was taken from the records retrospectively. Excluding patients who were seriously sick, since unable to get data during interview and lack of sufficient blood. The virological failure rates were assessed based on a single point testing of viral load, so there may be missed classification of HIV treatment failure. The prevalence of treatment failure is not really a populated-based estimate and is not representative of the broader population of people who are on ART and at risk of treatment failure. Despite these limitations, this study provides important information which would be useful for the ART treatment programs in the country.
The prevalence of treatment failure, immunological failure and virological failure among ART patients attending UOG referral hospital were 20.3, 13.2, and 14.7% respectively. Fifty-four (13%) patients were discordant. Even though no formal education, primary level education and duration on ART < 6 years were a significant risk factor of treatment failure, initial adult regimen D4T + 3TC+ EFV, AZT +3TC + NVP, AZT + 3TC + EFV, and TDF + 3TC + EFV were significantly protective. One of the following ART regimens: AZT +3TC + NVP, AZT + 3TC + EFV and TDF + 3TC + EFVare recommended. Switching as a result of toxicity was significant risk factor of immuno-virological discordance. Since it is single ART center study, the result may not also be generalized to all hospitals, so further study is needed to be done in a wider community and multiple ART centers to determine whether there are differences in virological and immunological responses and immune-virological discordance to antiretroviral therapy at different stages of HIV infection.
We would like to thanks the Department of Medical Microbiology School of Biomedical and Laboratory Science, Collage of Medicine and Health Science and University of Gondar for giving me this chance to conduct this research thesis.
Availability of data and materials
All the data (SPSS) sets generated and/or analyzed in this study are available from the corresponding author on reasonable request.
GA: study design, data collection, analysis and interpretation, and manuscript write-up. GY: the conception of the research idea and supervision, BT, AA, GF: study design, data analysis and interpretation and supervision. All authors have read and approved the final manuscript.
Ethics approval and consent to participate
Ethical clearance from the University of Gondar, School of Biomedical and Laboratory Sciences Ethical Review Committee was received and an official letter to the University of Gondar Referral Hospital administration prior to data collection with Ref. No SBMLS/ 678/ 09 was submitted. Written informed consent was received from every study participants following the elucidation of the reason and the aim of the research. The study participants who were not agreeing to participate in the research were not forced to participate. All received data and samples from the study participants were kept confidential by using codes as an alternative of any personal identifiers for the purpose of the research. The laboratory results from the study participants were talking to their physicians for the suitable treatment purpose.
Consent for publication
The authors declare that they have no competing interests.
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