Th17 cells reflect colon submucosal pathologic changes in active eosinophilic granulomatosis with polyangiitis
BMC Immunology volume 16, Article number: 75 (2015)
Chronic eosinophilic pneumonia (CEP) or eosinophilic gastroenteritis (EG), or both, with asthma precede the onset of eosinophilic granulomatosis with polyangiitis (EGPA) in half of all EGPA patients. It is not known what determines whether patients with CEP or with EG following asthma will develop EGPA.
We studied 17 EGPA patients and 12 patients with CEP but without EGPA. We assayed serum ICAM-1, VCAM-1, and VEGF, and the percentage of peripheral blood CD4+ T cells producing IL-17 (Th17 cells), at both onset and remission. We also examined the numbers of submucosal eosinophils and the basement membrane-to-crypt and crypt-to-crypt distance to evaluate edema in the colon submucosa at onset and remission in EGPA and at onset in CEP.
Nine of 12 (75.0 %) CEP patients had symptoms or endoscopic findings. Colonic submucosal eosinophil counts and edema in EGPA at onset were greater than at remission or in CEP at onset. Th17 cells (%) and serum ICAM-1 levels at onset were greater in EGPA than in CEP. In EGPA, peripheral blood Th17 cells (%) were significantly correlated with serum ICAM-1 level, colonic submucosal eosinophil count, and degree of edematous change; inversely correlated with serum VEGF level; but not correlated with VCAM-1 level.
Eosinophilia and colonic submucosal edematous change were greater in EGPA than in CEP. The mechanism of vasculitis in EGPA appears related to increases in serum Th17 cell numbers and ICAM-1 levels and decreases in VEGF levels.
Eosinophilic granulomatosis with polyangiitis (EGPA; formerly known as Churg-Strauss syndrome) is a rare disease characterized by allergic granulomatosis and necrotizing vasculitis developing after the appearance of peripheral and tissue eosinophilia . Asthma is present in 96 to 100 % of EGPA patients and is the cardinal feature of EGPA. Asthma may precede systemic vasculitis by approximately 8 years and in some cases by more than 30 years [1–3]. Eosinophilic infiltrations, such as those found in chronic eosinophilic pneumonia (CEP) or eosinophilic gastroenteritis (EG), or both, precede systemic vasculitis in half of all patients with EGPA [3, 4]. Some patients with asthma complicated by CEP are likely to develop EGPA if the CEP is left untreated . However, some patients in whom asthma is complicated by CEP do not develop EGPA. In our retrospective cohort study, we found that the clinical manifestations of asthma occurring in the pre-vasculitic phase of the disease were severe, or the percentage of eosinophils in the peripheral blood of pre-EGPA asthmatics at the first hospital visit was high, but bronchial hyperresponsiveness to acetylcholine in these pre-vasculitic patients was slight . We subsequently confirmed that maintenance of regulatory T cell (Treg cell) numbers in asthma patients with CEP may inhibit EGPA development via the action of cytokines, such as IL-10 and IL-2, produced by CD4+CD25+ and CD4+CD25− T cells, respectively . However, there is no clear mechanism that explains why some CEP patients with asthma develop EGPA and some do not.
Th17 cells play important roles in the immune response, depending on their stage of differentiation, be it induction, amplification, or stabilization. In mice, transforming growth factor beta (TGF-β) induces the development of inducible Treg (iTreg) cells by inducing the expression of the transcription factor forkhead box P3 (Foxp3) and the production of interleukin (IL)-17A by CD4+ T cells. IL-6, IL-1β, tumor necrosis factor alpha, and IL-21, together with TGF-β, promote Th17 differentiation or polarization . Some reports have suggested that Th17 cells are associated with vasculitis, as occurs in giant cell arteritis , Henoch-Schönlein purpura , antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis , granulomatosis polyangiitis or Wegener’s granulomatosis , and EGPA [13, 14].
Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM)-1 are expressed predominantly on the surfaces of endothelial cells and play an essential role in local leukocyte recruitment to the vessel wall, adhesion of leukocytes to the endothelium, migration, and extravasation [15, 16]. An enhanced level of soluble VCAM-1 (sVCAM-1) is a marker of endothelial cell activation in ANCA-associated vasculitides . Surface expression of ICAM-1, CD11b, and CD69 on eosinophils in the peripheral blood is increased in EGPA patients . Vascular endothelial growth factor (VEGF) is secreted by endothelial cells and pericytes in response to hypoxia. It induces angiogenesis and microvascular hyperpermeability . Serum VEGF levels are associated with disease activity, for example in ANCA-associated vasculitis  and systemic vasculitis .
Gastrointestinal tract involvement occurs in 17 to 92 % of patients with EGPA [1, 2, 21]. Characteristic symptoms in EGPA patients are abdominal pain, nausea or vomiting, diarrhea, hematochezia or melena, and hematemesis. Gastroduodenal ulceration has been detected endoscopically in 17 (27 %) of 62 patients with systemic vasculitis (SV) and colorectal ulceration in 6 (10 %), but in one study histologic signs of necrotizing vasculitis or granuloma could not be found in a number of small specimen . Patients with EGPA who have severe gastrointestinal tract involvement, such as surgical abdomen, can develop peritonitis, bowel perforation, gastrointestinal ischemia or infarction, and intestinal occlusion, which carry a poor prognosis; mesenteric vasculitis may occur, leading to gastrointestinal ulceration, ischemia, and perforation . Five-year survival rates in surgical patients with SV (EGPA patients were 11 of 62 patients , 82 of 342 patients ) have ranged from 41 to 56 % [22, 24]. Colonoscopic findings include mucosal erythema  and ulceration . However, in EGPA specially, the incidence of another minor endoscopic signs such as red flare, and edematous change is not known. Moreover, to our knowledge, there have been no published investigations of the pathology of necrotizing vasculitis with infiltration other than eosinophils or granuloma. There were various eosinophilic gastrointestinal diseases shown by drugs or parasitic infection, inflammatory bowel disease, reflux esophagitis, coeliac disease, microscopic and infectious colitis, SV (including EGPA), and polyarteritis nodosa [6, 26, 27]. It is not known whether the intestinal endoscopic findings characteristics of EG in the asthmatic phase precede SV in patients with EGPA. Moreover, the pathological differences in the colon submucosa between patients with EGPA and those with CEP or other types of EG have not yet been investigated.
Here, we examined the pathological differences related to numbers of eosinophils and amount of edema in the colon submucosa between patients with EGPA and those with CEP. We also analyzed the relationship between pathological features and adhesion molecule or growth factor expression and the Th17 response.
Clinical findings and treatment
There was no significant difference between patients with EGPA and those with CEP in terms of sex, atopic or non-atopic, presence or absence of allergic rhinitis or atopic dermatitis, age at time of onset of asthma, severity of asthma, and daily inhaled corticosteroid dose for treatment of asthma (Table 1). In five of 12 patients with CEP, eosinophilic pneumonitis improved without the patient taking systemic corticosteroids (i.e. prednisolone). However, all patients with EGPA received systemic corticosteroids (P < 0.01). The initial dose of prednisolone and the proportion of patients who used immunosuppressants or intravenous immunoglobulin (IVIG) were significantly higher in patients with EGPA than those in patients with CEP (Table 1; all P < 0.01). Our analysis of organ involvement revealed no significant differences between the two groups in terms of the rates of respiratory system involvement (asthma, paranasal sinusitis, and pulmonary infiltration) and the rates of involvement of the liver, gall bladder, and pancreas. EGPA patients had significantly higher rates of multiple polyneuropathy and myocardial, renal, skin, muscle, joint, and CNS involvement than CEP patients (P < 0.01 or P < 0.05).
Gastrointestinal tract characteristics in EGPA and CEP patients
The incidence of clinical symptoms related to both the upper and lower abdomen did not differ between the two groups (Table 1). There were various findings in the colonic mucosa (Fig. 1) as well as the gastro-duodenum mucosa (data not shown) in EGPA patients (ulceration, erosion, or dark red signs). In contrast, there were only slight changes in the colonic mucosa, such as red flare, in patients with CEP (and Fig. 1). However, the overall incidence of positive colonic mucosal findings on endoscopy did not differ between the two groups. In contrast, positive gastroscopic findings were more common in EGPA patients than those in CEP patients (P < 0.05, Table 1).
Counts and percentages of white blood cells (WBCs), eosinophils, and Th17 cells, and serum ICAM-1, VCAM-1, and VEGF levels
The numbers of WBCs and eosinophils in the peripheral blood at disease onset were significantly higher in EGPA patients than in CEP patients (P < 0.05 and P < 0.01, respectively; Table 2). Serum IgE radioimmunosorbent assay values did not differ between the two groups.
None of the CEP patients was positive for MPO- or PR3-ANCA. The serum ICAM-1 level at onset was greater in EGPA patients than in CEP patients (P < 0.05), but this was not the case for the serum VCAM-1 and VEGF levels. In EGPA patients, serum ICAM-1, VCAM-1, and VEGF levels were significantly higher at onset than at remission, but those in CEP patients did not change between onset and improvement. The percentage of CD4+ T cells producing IL-17 (so-called Th17 cells) at disease onset was significantly higher in patients with EGPA than in those with CEP (P < 0.01). The percentage of Th17 cells in EGPA patients was significantly lower at remission than at disease onset (P < 0.01), but there was no change in this percentage in CEP patients between onset and improvement (Table 2).
Pathological findings in the colon
We could not find any necrotizing vasculitis or granuloma in small size samples of submucosa from patients with either EGPA or CEP. At disease onset there were significantly more eosinophils in the colonic submucosa of EGPA patients than of CEP patients (P < 0.05) (Table 2, Fig. 2). Submucosal eosinophil counts in EGPA were significantly lower at remission than at onset (P < 0.05). The localization of submucosal edema varied within each patient (Fig. 3). The basement membrane-to-crypt interval and crypt-to-crypt distance reflected the degree of edema in the colonic submucosa. The basement membrane-to-crypt and crypt-to-crypt distance at disease onset were significantly greater in EGPA patients than in CEP patients (P < 0.01) (Fig. 2). In CEP patients, there were few, if any, edematous changes in the submucosa. The basement membrane-to-crypt and crypt-to-crypt distance in EGPA patients were significantly shorter at remission than at onset (P < 0.01) (Table 2).
Correlation between eosinophil counts in peripheral blood and colonic submucosa in patients with EGPA or CEP
The number of peripheral blood eosinophils in EGPA patients at both onset and remission was significantly correlated with the eosinophil count in the submucosa (P = 0.014, r = 0.42) (Fig. 4a), but this was not the case in CEP patients at onset (P = 0.42, r = 0.26) (data not shown).
Correlation between eosinophil count in colonic submucosa and basement membrane-to-crypt interval in patients with EGPA or CEP
The number of eosinophils in the colonic submucosa of EGPA patients at both onset and remission was significantly correlated with the basement membrane-to-crypt distance (P < 0.01, r = 0.61) (Fig. 4b), but this was not the case in CEP patients at onset (P = 0.37, r = –0.29) (data not shown). Similarly, the number of eosinophils in the colonic submucosa of EGPA patients was significantly correlated with the crypt-to-crypt distance (P < 0.01, r = 0.53), but this was not the case in CEP patients at disease (data not shown). The number of eosinophils in the peripheral blood of EGPA patients at onset and remission was significantly correlated with the crypt-to-crypt distance (P < 0.01, r = 0.52), but again this was not the case in CEP patients at disease onset (data not shown).
Correlations between percentage of Th17 cells in peripheral blood and eosinophil count in submucosa, crypt-to-crypt interval, and basement membrane-to-crypt interval in patients with EGPA
The percentage of Th17 cells in the peripheral blood of EGPA patients at onset and remission was significantly correlated with the submucosal eosinophil count (P < 0.01, r = 0.70) (Fig. 5a), with the crypt-to-crypt distance (P < 0.01, r = 0.53) (Fig. 5b), and with the basement membrane-to-crypt distance (P < 0.01, r = 0.55) (Fig. 5c).
Correlations between percentage of Th17 cells in peripheral blood and serum ICAM-1, VCAM-1, and VEGF levels in patients with EGPA
The percentage of Th17 cells in the peripheral blood of EGPA patients at onset and remission was significantly positively correlated with the serum ICAM-1 level (P < 0.05, r = 0.37) (Fig. 5d) and inversely correlated with the serum VEGF level (P < 0.05, r = – 0.40) (Fig. 5f), but it was not correlated with the serum VCAM-1 level (NS, r = 0.18) (Fig. 5e). The number of submucosal eosinophils was significantly correlated with the serum ICAM-1 level (P < 0.01, r = 0.44), but not with the serum VCAM-1 or VEGF level (data not shown). The number of eosinophils in the peripheral blood was significantly correlated with the serum ICAM-1 level (P < 0.05, r = 0.39) and VCAM-1 level (P < 0.01, r = 0.67), but not with the serum VEGF level (data not shown). The basement membrane-to-crypt distance was significantly correlated with the serum ICAM-1 level (P < 0.05, r = 0.44) but not with the serum VCAM-1 or VEGF level (data not shown).
The 5-year survival rate in EGPA is 62 to 97 % [21, 24, 28–30]. The prognosis of EGPA patients and the extent of mortality from the disease are associated with disease severity, as assessed by using the Five-Factor Scores developed in 1996  and 2009 . In addition, myocardial involvement  and the presence of gastrointestinal disease [2, 31] are independent negative prognostic factors. However, the gastrointestinal pathology of EGPA has not yet been fully characterized. Moreover, to our knowledge, differences in EG have not been investigated between patients with EGPA at disease onset and patients with asthma preceding the development of SV in the form of EGPA. Among the various eosinophilic gastrointestinal diseases [27, 32], there is less submucosal edema in ulcerative colitis, Crohn’s disease, and collagenous colitis [27, 33] than we saw here in our EGPA patients.
Until now there has been a lack of detailed information on pathological findings in the colonic submucosa. In our EGPA patients, we expected to find eosinophilia but were surprised to find no necrotizing vasculitis or granuloma. The presence of edematous change was also previously unreported. The eosinophil count in the submucosa was significantly correlated with the eosinophil count in the blood and with the basement membrane-to-crypt interval in patients with EGPA (Fig. 4), but not in patients with CEP (data not shown). The lack of correlation in the latter might have been caused by the fact that in these patients the colon was biopsied only once (at the onset of CEP), whereas in EGPA patients the colon was biopsied twice (at disease onset and at remission). All patients with asthma did not suffer eosinophilic gastrointestinal disorder. And there were an infiltration of eosinophils extravascular area in patients with asthma and without any gastrointestinal symptoms. Moreover there was an infiltration of number of eosinophils extra vascular extravascular tissue in patients with having more than 2052 number of eosinophils in peripheral blood .
We showed here that Th17 cell production decreased at remission in patients with EGPA. Previously, we showed that the count of iTreg cells producing IL-10 was reduced in EGPA patients at disease onset and upon relapse, but increased when the disease was inactive . Here, we investigated FOXP3+/CD4+ T cells as natural Treg cells in patients with EGPA or CEP. We confirmed that the percentage of Th17 cells was inversely correlated with the number of FOXP3+/CD4+ T cells (P < 0.01, r = -0.57) (data not shown). The number of FOXP3+/CD4+ T cells was significantly correlated with the number of eosinophils in the colonic submucosa (P < 0.01, r = 0.59) (data not shown), but unlike the Th17 cell count it was not correlated with the interval between basement membrane and crypt or between crypt and crypt (data not shown). This result indicated that the Th17 cell count but not the Treg cell count reflected the occurrence of pathological changes related to vasculitis. After treatment of EGPA patients, the serum ICAM-1 and VCAM-1 levels decreased significantly, whereas the serum VEGF level increased significantly (Table 2). The serum ICAM-1 level was significantly correlated with the VCAM-1 level (P = 0.011, r = 0.40) (data not shown). We found previously that multiple polyneuropathy and cardiac dysfunction improved after IVIG administration , and the numbers of Treg cells (as FOXP3+/CD4+ T cells or CD4+ T cells producing IL-10) increased for two years .
Maternal sera obtained after IVIG treatment, and polyclonal IVIG, decrease constitutive and cytokine-induced ICAM-1 and VCAM-1 expression on vascular endothelial cells ex vivo . The combination of IVIG and methylprednisolone generally has a greater suppressive effect on mRNA expression and on the production of VCAM-1, IL-1β, and VEGF on endothelial cells derived from human umbilical vein . IVIG-induced inhibition of angiogenesis has an inhibitory effect on VEGF-mediated blood perfusion in the ischemic limb in a mouse model . However, the positive clinical effects of high-dose IVIG on muscle function in patients with refractory inflammatory active myositis in the form of polymyositis or dermatomyositis are not accompanied by any effects on ICAM-1 and VCAM-1 . We considered that treatment with various combinations of corticosteroids, immunosuppressants, and IVIG induced clinical remission, decreased ICAM-1 and VCAM-1 levels, and increased VEGF levels. The sICAM-1 level was high in EGPA patients at disease onset, but the sVCAM-1 level varied widely and was relatively high at the time of onset of EGPA. An increase in Th17 cell numbers likely reflects active vasculitis , and an increase in the number of eosinophils or WBCs might induce upregulation of the expression of ICAM-1 or VCAM-1 on vascular endothelial cells; inflammatory cells such as eosinophils and lymphocytes, including Th17 cells, then migrate extravascularly into the colonic submucosa. An increase in serum VEGF level might have contributed to neovascularization.
We showed the correlation with Th17 cells in peripheral blood and ICAM-1 or VEGF in serum. We speculated the decrease of the number of Th17 cells after the treatment of steroid and immunosuppressant might affect the decrease of ICAM-1 and the increase of VEGF. These results were reflected to an inhibition of migration inflammatory cells and to promote neovascularization.
In summary, we clarified the pathological and immunological differences between patients with EGPA and those with CEP. We expect that this clarification will help us to diagnose EGPA in the early stages and to start treatment early, thus giving patients the chance of a good prognosis.
Between April 2008 and October 2012, we recruited 17 patients with EGPA and 12 patients with CEP but without EGPA. All patients with EGPA and CEP were recruited at the Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara, Kanagawa, Japan. EGPA was defined according to the classification criteria of the American College of Rheumatology . Patients with CEP were diagnosed with asthma by using the criteria of the Global Initiative for Asthma; the severity of asthma was also rated with the aid of these guidelines . CEP was defined as the fulfillment of at least two of three criteria, namely respiratory symptoms of more than 2 weeks’ duration; alveolar or blood eosinophilia (alveolar eosinophilia >25 % on bronchoalveolar lavage fluid differential cell count; blood eosinophilia >1000/mm3); and pulmonary infiltrates with a usually peripheral predominance on chest imaging . Patients with CEP who had allergic bronchopulmonary mycosis were excluded from the study.
Multiple mononeuritis—a measure of motor nerve dysfunction—was evaluated by using manual muscle testing; responses were scored from zero to five on the Medical Research Council scale. Sensory nerve dysfunction was evaluated by clinical examination. Lung involvement in EGPA patients was considered present when any of consolidation, ground grass opacity, nodules within such opacity, interlobular septal thickening, bronchial wall thickening, lymph node enlargement, pleural effusion evident upon high-resolution computer tomography, or eosinophil infiltration detected by lung biopsy was present. The heart was considered to be involved when any of chest pain, chest discomfort, back pain, palpitations, abnormal signs on cardiac echocardiography, Holter electrocardiographic abnormalities, elevated B-type natriuretic peptide levels, or [123I]-metaiodobenzylguanidine myocardial imaging abnormalities of the myocardium was evident . Gastrointestinal involvement was indicated by the presence of symptoms of epigastralgia, abdominal pain, diarrhea, or constipation, or of positive endoscopic signs, combined with confirmation of eosinophil infiltration by biopsy. Skin involvement was defined as the presence of purpura, erythema, livedo, or ulceration, or the presence of acrocyanosis when a nodule, accompanied by eosinophilic infiltration, was additionally detected by biopsy. Central nervous system involvement was defined as the presence of headache, visual disorder, abnormal visual sensation, cerebral infarction, bleeding, or cranial nerve dysfunction. Renal involvement was defined by any of the presence of eosinophils in the urine, glomerular nephritis, nephrosis, renal dysfunction (i.e., creatinine level >20 % above baseline), or proteinuria (>0.5 g/day).
All patients with EGPA were treated with conventional therapy (corticosteroids, immunosuppressants, or both; the initial dose of corticosteroid was approximately 1 mg/kg prednisolone daily for at least 1 month) . Remission was defined as the absence of any clinical signs or symptoms of active vasculitis for at least 6 months after these treatments. Patients with CEP who had not entered spontaneous remission received treatment with systemic corticosteroids in addition to inhaled corticosteroids.
At disease onset and at remission in patients with EGPA, and at disease onset and improvement in patients with CEP, we examined the whole blood cell count; eosinophil count in whole blood; serum IgE concentration; serum MPO- or PR3-ANCA level and ICAM-1, VCAM-1, and VEGF levels; and the percentage of CD4+ T cells producing IL-17 in the peripheral blood. Endoscopy of the large intestine was performed at onset and remission in patients with EGPA, and at onset in patients with CEP. The Ethics Committee of our NHO Sagamihara hospital approved the study, and written informed consent was obtained from each patient.
Endoscopy of the large intestine or the gastro-duodenum and biopsy of the colon mucosa
Endoscopy of the large intestine was performed after irrigation of the colon with 68 g of magnesium citrate dissolved in 1800 mL of water; each patient was anesthetized intravenously with 3 mg midazolam hydrochloride . A colonscope (Olympus CF-H290I, Olympus Optical Co., Tokyo, Japan) was inserted through the rectum and up into the sigmoid colon, ascending colon, transverse colon, descending colon, and ileocecum. Endoscopy of the gastro-duodenum was performed with using gastro fiberscope (Olympus GIF-XP260N, Olympus Optical Co., Tokyo, Japan). At least one point (the gastro-duodenum) or three points (the large intestine) on the mucous membrane were biopsied from both visible points and invisible findings.
Measurement of basement membrane-to-crypt interval, crypt-to-crypt interval, and numbers of eosinophils in submucosa
Biopsy specimens were fixed in 10 % formaldehyde, embedded in paraffin, cut at 3 μm, and stained with hematoxylin and eosin. To our knowledge, there is no accepted method for measuring the amount of edema in the colonic submucosa. We therefore chose to assess the grade of edema in the submucosa in accordance with the method used in a report by Sanderson et al. .
The crypt-to-crypt distance in the submucosa was measured as the closest distance between two crypts at 10 or more points in randomly selected fields of the biopsy specimen (Fig. 3). In the same way, the basement membrane-to-crypt distance was measured as the closest distance between basement membrane and crypt at 10 or more points. Similarly, the numbers of eosinophils in the submucosa were counted at five points or more in randomly selected fields of the biopsy specimen (Fig. 3). We used image analysis software (Image Pro Plus 6.0, Media Cybernetics, MD, USA) to evaluate edema and eosinophilic inflammation in the colonic submucosa. To determine the number of eosinophils, we counted the number of cells per slide and then converted that number to number of eosinophils/mm2. All values are expressed as means ± SD.
Induction of cytokine expression and staining of intracellular cytokines
Fluorescein isothiocyanate–bearing conjugates of mouse IgG1 and anti-CD4, and phycoerythrin (PE)-conjugated anti-human IL-17 were purchased from BioLegend or R&D Systems (Cosmo Bio Co. Ltd., Tokyo, Japan). To induce cytokine expression and accumulation, peripheral blood mononuclear cells (PBMCs, 1 × 106 cells/mL) suspended in RPMI 1640 medium supplemented with 10 % Fetal Calf Serum (FCS) were stimulated for 4 h at 37 °C with 10 μg/mL brefeldin A in the presence or absence of 50 ng/mL phorbol myristate acetate (PMA) and 1 μg/mL ionomycin . Cells that died after this stimulation were removed. The percentage of dead cells among PBMCs treated with PMA and ionomycin was calculated by exclusion-testing by staining with 0.4 % trypan blue solution. As a control we used whole blood cells diluted to the same extent in FCS-free RPMI 1640 before the addition of trypan blue. The percentage of dead cells was 6.8 ± 6.1 %. Dead cells stained with trypan blue could not be distinguished in the fluorescence-activated cell sorting (FACS). However, even if these dead cells were included, the statistical significance of the percentage of positive cells producing cytokines was unaffected.
Surface-stained whole-blood lymphocyte samples were suspended in 0.5 mL cold 4 % (v/v) paraformaldehyde (used as a fixative) and incubated at room temperature for 10 min. Next, the cells were washed twice with phosphate-buffered saline (PBS) and centrifuged at 200 g for 7 min. Each pellet thus obtained was suspended in 2 mL serum amyloid P component (SAP) buffer (0.1 % [w/v] saponin, 0.05 % [w/v] NaN3, in Hanks’s balanced salt solution). The cell suspension was again centrifuged at 200 g for 7 min, and the cell pellet was suspended in 0.1 mL SAP buffer. Cell suspensions were diluted with PBS and aliquoted into tubes containing 106 cells/20 μL. Phycoerythrin-conjugated anti-IL-17 was added to each tube. All tubes were vortexed and incubated for 35 min at room temperature in the dark. The percentage of cells generating cytokines was measured with a FACSCalibur (Nippon Becton Dickinson, Tokyo, Japan) flow cytometry system and the data were analyzed with the aid of CELLQuest software (Nippon Becton Dickinson, Tokyo, Japan).
Soluble ICAM-1, sVCAM-1, and VEGF levels in serum
Soluble ICAM-1 (sICAM-1), sVCAM-1, and VEGF levels in the serum were assayed by using Human soluble ICAM-1, VCAM-1, and VEGF Platinum ELISA kits (eBioscience, San Diego, CA, USA). For quantification of sICAM-1 and sVCAM-1, those plates were washed twice with 400 μg of wash buffer (PBS with 0.05 % Tween 20). Then 100 μL of Assay Buffer and 100 μL of diluted plasma (in the case of VCAM-1) or 10 μL of sample (in the case of ICAM-1) was incubated in each plate well. Fifty micrograms of conjugate mixture (biotin-conjugated anti-human sICAM-1 or sVCAM-1) was added to the well, which was then incubated for 1 h (sICAM-1) or 2 h (sVCAM-1) at room temperature. After each well had been washed three times, 100 mL of tetramethylbenzidine (TMB) Substrate Solution was added to each well and the mixture was incubated for 10 min at room temperature. A stop solution (1 M phosphoric acid) was then added and the results analyzed by using an ELISA reader.
For quantification of VEGF, plates were first washed twice with 400 μg of wash buffer, then 50 μL of Assay Buffer and 50 μL of sample were incubated in each plate well for 2 h at room temperature. One hundred micrograms of conjugate mixture (biotin-conjugated anti-human VEGF-A) was added and the well was incubated for 1 h at room temperature. After the wells had been washed six times, 100 mL of TMB Substrate Solution was added to each well and the mixture incubated for 30 min at room temperature. After another six washings of the wells, VEGF levels were analyzed with an ELISA reader after addition of the stop solution as above.
All values are expressed as means ± SDs unless otherwise specified. Statistical comparisons among groups were achieved by using two-way analysis of variance (ANOVA) employing a repeated-measures algorithm, followed by post-hoc comparisons using the Newman-Keuls test. Two mean values were compared by using the Mann-Whitney U-test. The two mean values obtained by this process were compared by using the Wilcoxon matched-pairs T-test. Correlation coefficients were obtained by employing Spearman’s rank correlation test. P values <0.05 were considered statistically significant. Statistical analysis was performed with SPSS for Windows, version 20 (SPSS Inc., Chicago, IL).
Antineutrophil cytoplasmic antibody
Chronic eosinophilic pneumonia
Eosinophilic granulomatosis with polyangiitis
Fluorescence-activated cell sorting
Forkhead box P3
Intercellular adhesion molecule-1
Phorbol myristate acetate
Peripheral blood mononuclear cell
Serum amyloid P
- Treg cell:
Regulatory T cell
Soluble intercellular adhesion molecule-1
Soluble vascular cell adhesion molecule-1
Transforming growth factor beta
Vascular cell adhesion molecule-1
Vascular endothelial growth factor
White blood cell
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We thank Dr. Yukio Watanabe, Dr. Yuta Matsumoto, Dr. Aya Irie, Dr. Takayuki Irie for performing the endoscopies of the large intestine.
No author has any conflict of interest to disclose. The work was not funded by a grant or any other external source of financial support. N. Tsurikisawa MD, C. Oshikata MD, T. Tsuburai MD, S. Sugano MD, Y. Nakamura MD, T. Shimoda MD, S. Tamama MD, K. Adachi MD, A. Horita MD, I. Saito MD, H. Saito PhD.
NT participated in study design and medical examination of patients, analyzed the data and statistics, was the main contributor to manuscript preparation, and was involved in manuscript preparation and editing. CO examined the patients and contributed to discussions about the patients. TT contributed to discussions about the patients. SS, YN, TS, ST, and KA performed endoscopies of the large intestine in the patients. AH and IS advised on the pathological analysis and contributed to discussions about the manuscript. HS assayed serum ICAM-1, VCAM-1, and VEGF, and analyzed Th17 cells by FACS. All authors read and approved the final manuscript.
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Tsurikisawa, N., Oshikata, C., Tsuburai, T. et al. Th17 cells reflect colon submucosal pathologic changes in active eosinophilic granulomatosis with polyangiitis. BMC Immunol 16, 75 (2015). https://doi.org/10.1186/s12865-015-0138-4
- Churg-Strauss syndrome
- Eosinophilic granulomatosis with polyangiitis
- Intercellular adhesion molecule-1
- Vascular cell adhesion molecule-1
- Vascular endothelial growth factor